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Protein

Activating signal cointegrator 1 complex subunit 3

Gene

Ascc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi500 – 507ATPPROSITE-ProRule annotation8
Nucleotide bindingi1350 – 1357ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: UniProtKB
  • ATP-dependent helicase activity Source: GO_Central
  • nucleic acid binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Activating signal cointegrator 1 complex subunit 3 (EC:3.6.4.12By similarity)
Gene namesi
Name:Ascc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1925237 Ascc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004169151 – 2198Activating signal cointegrator 1 complex subunit 3Add BLAST2198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineBy similarity1
Modified residuei573N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiE9PZJ8
MaxQBiE9PZJ8
PaxDbiE9PZJ8
PeptideAtlasiE9PZJ8
PRIDEiE9PZJ8

PTM databases

iPTMnetiE9PZJ8
PhosphoSitePlusiE9PZJ8

Expressioni

Gene expression databases

BgeeiENSMUSG00000038774 Expressed in 232 organ(s), highest expression level in lung
ExpressionAtlasiE9PZJ8 baseline and differential
GenevisibleiE9PZJ8 MM

Interactioni

Subunit structurei

Identified in the ASCC complex that contains ASCC1, ASCC2 and ASCC3. Functions as scaffolding subunit that interacts directly with both ASCC1 and ASCC2. Interacts directly with ALKBH3, and thereby recruits ALKBH3 to the ASCC complex. Part of the ASC-1/TRIP4 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3.By similarity

Protein-protein interaction databases

BioGridi219071, 2 interactors
IntActiE9PZJ8, 1 interactor
STRINGi10090.ENSMUSP00000036726

Structurei

3D structure databases

SMRiE9PZJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini487 – 670Helicase ATP-binding 1PROSITE-ProRule annotationAdd BLAST184
Domaini697 – 915Helicase C-terminal 1PROSITE-ProRule annotationAdd BLAST219
Domaini979 – 1288SEC63 1Add BLAST310
Domaini1337 – 1512Helicase ATP-binding 2PROSITE-ProRule annotationAdd BLAST176
Domaini1545 – 1740Helicase C-terminal 2PROSITE-ProRule annotationAdd BLAST196
Domaini1813 – 2177SEC63 2Add BLAST365

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili18 – 81Sequence analysisAdd BLAST64
Coiled coili328 – 356Sequence analysisAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi612 – 615DEVH box4
Motifi1454 – 1457DEIH box4

Sequence similaritiesi

Belongs to the helicase family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0952 Eukaryota
COG1204 LUCA
GeneTreeiENSGT00640000091272
HOGENOMiHOG000157749
HOVERGENiHBG071596
InParanoidiE9PZJ8
KOiK18663
OMAiYKTLNRM
OrthoDBiEOG091G0HE5
TreeFamiTF105778

Family and domain databases

CDDicd00079 HELICc, 2 hits
Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR035892 C2_domain_sf
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR014756 Ig_E-set
IPR027417 P-loop_NTPase
IPR004179 Sec63-dom
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 2 hits
PF00271 Helicase_C, 2 hits
PF02889 Sec63, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM00487 DEXDc, 2 hits
SM00490 HELICc, 2 hits
SM00973 Sec63, 2 hits
SUPFAMiSSF46785 SSF46785, 2 hits
SSF52540 SSF52540, 4 hits
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 2 hits
PS51194 HELICASE_CTER, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: E9PZJ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALPRLTGAL RSFSNVTKQD NYNEEVADLK LKRSKLHEQV LDFGLTWKKI
60 70 80 90 100
VKFLNEKLEK NKMQNINEDL KDILQAAKQI VGTDNGREAI ESGAAFLFMT
110 120 130 140 150
FHMTDSVGYM ETKAIRQTFG PFPSSSATSA CNATNRIISH FSQDDLTAFV
160 170 180 190 200
QMAENPCNDR VVFGKNLAFS FDMYDLDHFD ELPINGESQK TISLDYKKFL
210 220 230 240 250
NEQFQEPYTP ELKPVEKTNG SLLWCEVEKY LNATLKEMTE AARVEDLCCT
260 270 280 290 300
LYDMLASAKS GDELQDELFE LLGPEGLDLI EKLLQNRITI VDRFLNSSSD
310 320 330 340 350
HKFQVLQDSC KKILGENSKP NYGCQVTIQS EQEKQLMKQY RREEKRIARR
360 370 380 390 400
EKKAGEDGEV SGEGVLPFDP KELRIQREHA LLNARNAPIL GRQRDVEFEK
410 420 430 440 450
IRYPHVYDSQ AQARETSAFI AGAKMILPEG IQRENTKLYE EVRIPYGEPM
460 470 480 490 500
PVGFEEKPVY IKDLDEVGQL AFKGMKRLNR IQSIVFETAY NTNENMLICA
510 520 530 540 550
PTGAGKTNIA MLTILHEIRQ HFHQGVIKKN EFKIVYVAPM KALAAEMTNY
560 570 580 590 600
FSKRLEPLGI VVKELTGDMQ LSKSEILRTQ MLVTTPEKWD VVTRKSVGDV
610 620 630 640 650
ALSQIVKLLI LDEVHLLHED RGPVLESIVA RTLRQVESTQ SMIRILGLSA
660 670 680 690 700
TLPNYLDVAT FLHVNPYIGL FYFDGRFRPV PLGQTFLGIK STNKMQQLNN
710 720 730 740 750
MDEVCYESVL KQVKAGHQVM VFVHARNATV RTAMSLIERA KNSGQISCFL
760 770 780 790 800
PTEGPEYGHA LKQVQKSRNK QVRELFSDGF SIHHAGMLRQ DRNLVENLFS
810 820 830 840 850
NGHIKVLVCT ATLAWGVNLP AHAVVIKGTQ IYAAKRGSFV DLGILDVMQI
860 870 880 890 900
FGRAGRPQFD KFGEGIIITT HDKLSHYLSL LTQQNPIESQ FLESLADNLN
910 920 930 940 950
AEIALGTVTN VEEAVRWMSY TYLYVRMRAN PLAYGISHKA YQIDPTLRKH
960 970 980 990 1000
REQLLIEVGQ KLDKAKMIRF EERTGYFSST DLGRTASHFY IKYNTIETFN
1010 1020 1030 1040 1050
ELFDAHKTEG DIFAIVSKAE EFDQIKVREE EIEELDALLN NFCELSAPGG
1060 1070 1080 1090 1100
VENSYGKINI LLQTYISRGE MDSFSLISDS AYVAQNAARI VRALFEIALR
1110 1120 1130 1140 1150
KRWPTMTYRL LNLSKVIDKR LWGWASPLRQ FSVLPPHILT RLEEKNLTVD
1160 1170 1180 1190 1200
KLKDMRKDEI GHILHHVNIG LKVKQCVHQI PSVTMEASIQ PITRTVLRVS
1210 1220 1230 1240 1250
LNIHPDFSWN DQVHGTVGEP WWIWVEDPTN DHIYHSEYFL ALKKQVINKE
1260 1270 1280 1290 1300
AQLLVFTIPI FEPLPSQYYI RAVSDRWLGA EAVCIINFQH LILPERHPPH
1310 1320 1330 1340 1350
TELLDLQPLP ITALGCKAYE ALYNFSHFNP VQTQIFHTLY HTDCNVLLGA
1360 1370 1380 1390 1400
PTGSGKTVAA ELAIFRVFNK YPTSKAVYIA PLKALVRERM DDWKIRIEEK
1410 1420 1430 1440 1450
LGKKVIELTG DVTPDMKSIA KADLIVTTPE KWDGVSRSWQ NRSYVQQVNI
1460 1470 1480 1490 1500
LIIDEIHLLG EERGPVLEVI VSRTNFISSH TEKPVRIVGL STALANARDL
1510 1520 1530 1540 1550
ADWLNIKQMG LFNFRPSVRP VPLEVHIQGF PGQHYCPRMA SMNKPAFQAI
1560 1570 1580 1590 1600
RSHSPAKPVL IFVSSRRQTR LTALELIAFL ATEEDPKQWL NMDEQEMDNI
1610 1620 1630 1640 1650
IGTVRDSNLK LTLAFGIGMH HAGLHERDRK TVEELFVNCK VQVLIATSTL
1660 1670 1680 1690 1700
AWGVNFPAHL VIIKGTEYYD GKTRRYVDFP ITDVLQMMGR AGRPQFDDQG
1710 1720 1730 1740 1750
KAVILVHDIK KDFYKKFLYE PFPVESSLLG VLSDHLNAEI AGGTITSKQD
1760 1770 1780 1790 1800
AMDYITWTYF FRRLIMNPSY YSLGDVSQDS INKFLSHLIG QSLVELELSH
1810 1820 1830 1840 1850
CIEVGEDNRT IEPLTCGRIA SYYYLKHKTV KMFKDRLKPE CSTEELLSIL
1860 1870 1880 1890 1900
SDAEEYTDLP VRHNEDHTNN ELAKCLPIEL NPHSFDSPHT KAHLLLQAHL
1910 1920 1930 1940 1950
SRAMLPCPDY DTDTKTVLDQ ALRVCQAMLD VAASQGWLVT VLNITHLIQM
1960 1970 1980 1990 2000
VIQGRWLKDS SLLTIPNIEQ HHLHLFRKWK PPVKSSHAKC RTSIECLPEL
2010 2020 2030 2040 2050
IHACEGKDHV FSSMVEKELQ PAKTKQAWNF LSRLPVINVG ISVKGSWDDL
2060 2070 2080 2090 2100
VEGHNELSIS TLTADKRDEN KWIKLHADQE YVLQVSLQRV HFGLPKGKHE
2110 2120 2130 2140 2150
NHAVTPRFPK LKDEGWFLIL GEVDKRELMA VKRVGFVRTH HDASISFFTP
2160 2170 2180 2190
ETPGRYIFTL YLMSDCYLGL DQQYDIYLNV IKANISTKDS DVFTDLSV
Length:2,198
Mass (Da):250,557
Last modified:April 5, 2011 - v1
Checksum:i39FF851ED47394FD
GO
Isoform 2 (identifier: E9PZJ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1070: Missing.

Note: No experimental confirmation available.
Show »
Length:1,128
Mass (Da):128,982
Checksum:iF7057317EB545F77
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P6I6A0A1W2P6I6_MOUSE
Activating signal cointegrator 1 co...
Ascc3
334Annotation score:
A0A1W2P891A0A1W2P891_MOUSE
Activating signal cointegrator 1 co...
Ascc3
48Annotation score:

Sequence cautioni

The sequence AAH32189 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1818R → L in AAH59917 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0429991 – 1070Missing in isoform 2. 1 PublicationAdd BLAST1070

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137877 Genomic DNA No translation available.
AC153529 Genomic DNA No translation available.
AC159114 Genomic DNA No translation available.
BC032189 mRNA Translation: AAH32189.1 Different initiation.
BC059917 mRNA Translation: AAH59917.1
AK021027 mRNA Translation: BAC25644.1
AK052745 mRNA Translation: BAC35127.1
CCDSiCCDS48555.1 [E9PZJ8-1]
RefSeqiNP_932124.2, NM_198007.2 [E9PZJ8-1]
UniGeneiMm.222497

Genome annotation databases

EnsembliENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774 [E9PZJ8-1]
GeneIDi77987
KEGGimmu:77987
UCSCiuc007fak.2 mouse [E9PZJ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137877 Genomic DNA No translation available.
AC153529 Genomic DNA No translation available.
AC159114 Genomic DNA No translation available.
BC032189 mRNA Translation: AAH32189.1 Different initiation.
BC059917 mRNA Translation: AAH59917.1
AK021027 mRNA Translation: BAC25644.1
AK052745 mRNA Translation: BAC35127.1
CCDSiCCDS48555.1 [E9PZJ8-1]
RefSeqiNP_932124.2, NM_198007.2 [E9PZJ8-1]
UniGeneiMm.222497

3D structure databases

SMRiE9PZJ8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219071, 2 interactors
IntActiE9PZJ8, 1 interactor
STRINGi10090.ENSMUSP00000036726

PTM databases

iPTMnetiE9PZJ8
PhosphoSitePlusiE9PZJ8

Proteomic databases

EPDiE9PZJ8
MaxQBiE9PZJ8
PaxDbiE9PZJ8
PeptideAtlasiE9PZJ8
PRIDEiE9PZJ8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035606; ENSMUSP00000036726; ENSMUSG00000038774 [E9PZJ8-1]
GeneIDi77987
KEGGimmu:77987
UCSCiuc007fak.2 mouse [E9PZJ8-1]

Organism-specific databases

CTDi10973
MGIiMGI:1925237 Ascc3

Phylogenomic databases

eggNOGiKOG0952 Eukaryota
COG1204 LUCA
GeneTreeiENSGT00640000091272
HOGENOMiHOG000157749
HOVERGENiHBG071596
InParanoidiE9PZJ8
KOiK18663
OMAiYKTLNRM
OrthoDBiEOG091G0HE5
TreeFamiTF105778

Miscellaneous databases

PROiPR:E9PZJ8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038774 Expressed in 232 organ(s), highest expression level in lung
ExpressionAtlasiE9PZJ8 baseline and differential
GenevisibleiE9PZJ8 MM

Family and domain databases

CDDicd00079 HELICc, 2 hits
Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR035892 C2_domain_sf
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR014756 Ig_E-set
IPR027417 P-loop_NTPase
IPR004179 Sec63-dom
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 2 hits
PF00271 Helicase_C, 2 hits
PF02889 Sec63, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM00487 DEXDc, 2 hits
SM00490 HELICc, 2 hits
SM00973 Sec63, 2 hits
SUPFAMiSSF46785 SSF46785, 2 hits
SSF52540 SSF52540, 4 hits
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 2 hits
PS51194 HELICASE_CTER, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiASCC3_MOUSE
AccessioniPrimary (citable) accession number: E9PZJ8
Secondary accession number(s): Q6PB36
, Q8C1G1, Q8C707, Q8K292
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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