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Protein

Katanin p60 ATPase-containing subunit A1

Gene

Katna1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Activity regulationi

ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi251 – 258ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processCell cycle, Cell division, MitosisUniRule annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p60 ATPase-containing subunit A1UniRule annotation (EC:3.6.4.3UniRule annotation)
Short name:
Katanin p60 subunit A1UniRule annotation
Alternative name(s):
p60 kataninUniRule annotation
Gene namesi
Name:Katna1Imported
Synonyms:KATNA1UniRule annotation
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1344353 Katna1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, CytoskeletonUniRule annotation, MicrotubuleUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei44Phosphoserine; by DYRK2UniRule annotation1

Post-translational modificationi

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.UniRule annotation
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.UniRule annotation

Keywords - PTMi

PhosphoproteinUniRule annotation, Ubl conjugationUniRule annotation

Proteomic databases

MaxQBiE9PZI6
PeptideAtlasiE9PZI6
PRIDEiE9PZI6

Expressioni

Gene expression databases

BgeeiENSMUSG00000019794 Expressed in 274 organ(s), highest expression level in cochlea
ExpressionAtlasiE9PZI6 baseline and differential
GenevisibleiE9PZI6 MM

Interactioni

Subunit structurei

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM. Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42. Interacts with KATNB1 and KATNBL1. Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

IntActiE9PZI6, 12 interactors

Structurei

3D structure databases

ProteinModelPortaliE9PZI6
SMRiE9PZI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini243 – 385AAAInterPro annotationAdd BLAST143

Domaini

The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0738 Eukaryota
ENOG410XPN7 LUCA
GeneTreeiENSGT00550000074466
KOiK07767
OrthoDBiEOG091G0Q8J
TreeFamiTF323170

Family and domain databases

HAMAPiMF_03023 Katanin_p60_A1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR028596 KATNA1
IPR036181 MIT_dom_sf
IPR027417 P-loop_NTPase
IPR015415 Vps4_C
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF09336 Vps4_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF116846 SSF116846, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

E9PZI6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTMSLQMIVE NVKLAREYAL LGNYDSAMVY YQGVLDQMNK YLYSVKDTHL
60 70 80 90 100
RQKWQQVWQE INVEAKQVKD IMKTLESFKL DITSLQAAQH EFPAAEGEVW
110 120 130 140 150
SLPVPVERRP LPGPRKRQSS QHSDPKPHSN RPSTVVRAHR PSPQNLHNDR
160 170 180 190 200
GKAVRSREKK EQSKGREEKN KLPAAVTEPE ANKFDGTGYD KDLVEALERD
210 220 230 240 250
IISQNPNVRW YDIADLVEAK KLLQEAVVLP MWMPEFFKGI RRPWKGVLMV
260 270 280 290 300
GPPGTGKTLL AKAVATECKT TFFNVSSSTL TSKYRGESEK LVRLLFEMAR
310 320 330 340 350
FYSPATIFID EIDSICSRRG TSEEHEASRR MKAELLVQMD GVGGASENDD
360 370 380 390 400
PSKMVMVLAA TNFPWDIDEA LRRRLEKRIY IPLPSAKGRE ELLRISLREL
410 420 430 440 450
ELADDVNLAS IAENMEGYSG ADITNVCRDA SLMAMRRRIE GLTPEEIRNL
460 470 480 490
SREAMHMPTT MEDFEMALKK ISKSVSAADI ERYEKWIVEF GSC
Length:493
Mass (Da):56,215
Last modified:April 5, 2011 - v1
Checksum:iA579AD217928E603
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9WV86KTNA1_MOUSE
Katanin p60 ATPase-containing subun...
Katna1
491Annotation score:
E9QKG2E9QKG2_MOUSE
Katanin p60 ATPase-containing subun...
Katna1 KATNA1
491Annotation score:
G3UWK1G3UWK1_MOUSE
Katanin p60 ATPase-containing subun...
Katna1
66Annotation score:
G3UX83G3UX83_MOUSE
Katanin p60 ATPase-containing subun...
Katna1
130Annotation score:
G3UYX0G3UYX0_MOUSE
Katanin p60 ATPase-containing subun...
Katna1
54Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC156391 Genomic DNA No translation available.
RefSeqiNP_035965.2, NM_011835.2
UniGeneiMm.28127
Mm.421297

Genome annotation databases

EnsembliENSMUST00000165806; ENSMUSP00000132514; ENSMUSG00000019794
GeneIDi23924
KEGGimmu:23924
UCSCiuc007eii.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC156391 Genomic DNA No translation available.
RefSeqiNP_035965.2, NM_011835.2
UniGeneiMm.28127
Mm.421297

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OW5X-ray1.70B/D1-1[»]
B/D3-80[»]
ProteinModelPortaliE9PZI6
SMRiE9PZI6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiE9PZI6, 12 interactors

Proteomic databases

MaxQBiE9PZI6
PeptideAtlasiE9PZI6
PRIDEiE9PZI6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000165806; ENSMUSP00000132514; ENSMUSG00000019794
GeneIDi23924
KEGGimmu:23924
UCSCiuc007eii.2 mouse

Organism-specific databases

CTDi11104
MGIiMGI:1344353 Katna1

Phylogenomic databases

eggNOGiKOG0738 Eukaryota
ENOG410XPN7 LUCA
GeneTreeiENSGT00550000074466
KOiK07767
OrthoDBiEOG091G0Q8J
TreeFamiTF323170

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019794 Expressed in 274 organ(s), highest expression level in cochlea
ExpressionAtlasiE9PZI6 baseline and differential
GenevisibleiE9PZI6 MM

Family and domain databases

HAMAPiMF_03023 Katanin_p60_A1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR028596 KATNA1
IPR036181 MIT_dom_sf
IPR027417 P-loop_NTPase
IPR015415 Vps4_C
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF09336 Vps4_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF116846 SSF116846, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE9PZI6_MOUSE
AccessioniPrimary (citable) accession number: E9PZI6
Entry historyiIntegrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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