UniProtKB - E9PXF8 (MTMRD_MOUSE)
Myotubularin-related protein 13
Sbf2
Functioni
Guanine nucleotide exchange factor (GEF) which activates RAB21 and possibly RAB28 (By similarity).
Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity).
In response to starvation-induced autophagy, activates RAB21 which in turn binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion (By similarity).
Acts as an adapter for the phosphatase MTMR2 (PubMed:16399794).
Increases MTMR2 catalytic activity towards phosphatidylinositol 3,5-bisphosphate and to a lesser extent towards phosphatidylinositol 3-phosphate (PubMed:16399794).
By similarity1 PublicationCaution
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: MGI
- identical protein binding Source: MGI
- phosphatase binding Source: MGI
- phosphatase regulator activity Source: MGI
- phosphatidylinositol binding Source: MGI
GO - Biological processi
- autophagy Source: UniProtKB-KW
- regulation of GTPase activity Source: InterPro
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Biological process | Autophagy |
Enzyme and pathway databases
Reactomei | R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs |
Names & Taxonomyi
Protein namesi | Recommended name: Myotubularin-related protein 13CuratedAlternative name(s): Inactive phosphatidylinositol 3-phosphatase 13Curated SET-binding factor 2Imported |
Gene namesi | Name:Sbf2Imported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921831, Sbf2 |
VEuPathDBi | HostDB:ENSMUSG00000038371 |
Subcellular locationi
Endosome
- Endosome membrane 1 Publication; Peripheral membrane protein Curated
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Other locations
- perinuclear region 1 Publication
- Membrane By similarity; Peripheral membrane protein By similarity
- axon 1 Publication
Note: Associated with membranes (By similarity). Localizes to vacuoles in hypo-osmotic conditions (PubMed:16399794). Membrane localization is likely to be mediated via its interaction with MTMR2 (PubMed:23297362).By similarity2 Publications
Cytosol
- cytosol Source: Reactome
Endosome
- endosome membrane Source: UniProtKB-SubCell
Other locations
- axon Source: UniProtKB-SubCell
- membrane Source: MGI
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- vacuolar membrane Source: MGI
Keywords - Cellular componenti
Cell projection, Cytoplasm, Endosome, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000446366 | 1 – 1872 | Myotubularin-related protein 13Add BLAST | 1872 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1125 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1302 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | E9PXF8 |
MaxQBi | E9PXF8 |
PaxDbi | E9PXF8 |
PeptideAtlasi | E9PXF8 |
ProteomicsDBi | 346740 347539 [E9PXF8-1] |
PTM databases
iPTMneti | E9PXF8 |
PhosphoSitePlusi | E9PXF8 |
SwissPalmi | E9PXF8 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000038371, Expressed in sciatic nerve and 311 other tissues |
Genevisiblei | E9PXF8, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:16399794). Heterotetramer consisting of one MTMR2 dimer and one SBF2/MTMR13 dimer (PubMed:16399794).
Interacts with class II PI3-kinase PIK3C2A (PubMed:22648168).
Interacts (via DENN domain) with RAB21 (in GDP-bound form) in response to starvation; the interaction activates RAB21 (By similarity).
Interacts with VAMP8 in response to starvation (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- phosphatase binding Source: MGI
Protein-protein interaction databases
IntActi | E9PXF8, 1 interactor |
STRINGi | 10090.ENSMUSP00000033058 |
Miscellaneous databases
RNActi | E9PXF8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 172 | uDENNPROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 191 – 324 | cDENNPROSITE-ProRule annotationAdd BLAST | 134 | |
Domaini | 326 – 427 | dDENNPROSITE-ProRule annotationAdd BLAST | 102 | |
Domaini | 869 – 1004 | GRAMSequence analysisAdd BLAST | 136 | |
Domaini | 1106 – 1607 | Myotubularin phosphatasePROSITE-ProRule annotationAdd BLAST | 502 | |
Domaini | 1766 – 1870 | PHPROSITE-ProRule annotationAdd BLAST | 105 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1652 – 1705 | Required for homodimerization and interaction with MTMR2By similarity1 PublicationAdd BLAST | 54 | |
Regioni | 1697 – 1720 | DisorderedSequence analysisAdd BLAST | 24 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1090, Eukaryota KOG4471, Eukaryota |
GeneTreei | ENSGT00940000155385 |
HOGENOMi | CLU_002298_1_1_1 |
InParanoidi | E9PXF8 |
OMAi | QQFWEDA |
OrthoDBi | 45015at2759 |
PhylomeDBi | E9PXF8 |
TreeFami | TF318583 |
Family and domain databases
CDDi | cd13339, PH-GRAM_MTMR13, 1 hit |
Gene3Di | 2.30.29.30, 2 hits 3.40.50.11500, 1 hit |
InterProi | View protein in InterPro IPR001194, cDENN_dom IPR005112, dDENN_dom IPR043153, DENN_C IPR004182, GRAM IPR030567, MTMR13 IPR037823, MTMR13_PH-GRAM IPR010569, Myotubularin-like_Pase_dom IPR030564, Myotubularin_fam IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR029021, Prot-tyrosine_phosphatase-like IPR022096, SBF1/SBF2 IPR037516, Tripartite_DENN IPR005113, uDENN_dom |
PANTHERi | PTHR10807, PTHR10807, 1 hit PTHR10807:SF4, PTHR10807:SF4, 1 hit |
Pfami | View protein in Pfam PF02141, DENN, 1 hit PF02893, GRAM, 1 hit PF06602, Myotub-related, 1 hit PF00169, PH, 1 hit PF12335, SBF2, 1 hit PF03456, uDENN, 1 hit |
SMARTi | View protein in SMART SM00801, dDENN, 1 hit SM00799, DENN, 1 hit SM00568, GRAM, 1 hit SM00233, PH, 1 hit SM00800, uDENN, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS50211, DENN, 1 hit PS50003, PH_DOMAIN, 1 hit PS51339, PPASE_MYOTUBULARIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MARLADYFIV VGYDHEKPAG PGEGLGKIIQ RFPQQDWDDT PFPQGIELFC
60 70 80 90 100
QPGGWHLSRE RKQPTFFVVV LTDIDSDRHY CSCLTFYEAE INLQGTKKEE
110 120 130 140 150
IEGEEVSGLI QPAEVFAPKS LVLVSRLDYP EIFRACLGLI YTVYVDSMSV
160 170 180 190 200
SLESLIANLC ACLVPAAGGS QKLFSLGAGD RQLIQTPLHD SLPVTGTSVA
210 220 230 240 250
LLFQQLGIQN VLNLFCAVLT ENKVLFHSAS FQRLSDACRA LESLMFPLKY
260 270 280 290 300
SYPYIPILPA QLLEVLSSPT PFIIGVHSIF KTDVHELLDV IIADLDGGTI
310 320 330 340 350
KIPECIHLSS LPEPLLHQTQ SALSLILHPD LEVADHAFPP PRTALSHSKM
360 370 380 390 400
LDKEVRAVFL RLFAQLFQGY RSCLQLIRIH AEPVIHFHKT AFLGQRGLVE
410 420 430 440 450
NDFLTKVLNG MAFAGFVSER GPPYRACDLF DELVAFEVER IKVEEKNPLK
460 470 480 490 500
MIKHIRELAE QLFKNENPNP HMAFQKVPRP TEGSHLRVHI LPFPKINEAR
510 520 530 540 550
VQELIQENLA KNQNAPPATR IEKKCVVPAG PPVVSIMEKV ITVFNSAQRL
560 570 580 590 600
EVVRNCISFI FENKTLETEK TLPAALRALK GKAARQCLTD ELGLHVQQNR
610 620 630 640 650
AILDHQQFDY IIRMMNCTLQ DCSSLEEYNI AAALLPLTSA FYRKLAPGVS
660 670 680 690 700
QFAYTCVQDH PIWTNQQFWE TTFYNAVQEQ VRSLYLSAKD DNHIPHLKQK
710 720 730 740 750
LPDGQHQEKT AMDLAAEQLR LWPTLSKSTQ QELVQHEEST VFSQAIHFAN
760 770 780 790 800
LMVNLLVPLD TSKNKLLRAS APGDWESGSN SIVTNSIAGS VAESYDTESG
810 820 830 840 850
FEDSENSDVA NSVVRFIARF IDKVCTESGV TQDHIRSLHC MIPGIVAMHI
860 870 880 890 900
ETLEAVHRES RRLPPIQKPK ILRPALLPGE EIVCEGLRVL LDPDGREEAT
910 920 930 940 950
GGLLGGPQLL PAEGALFLTT YRILFRGTPH DQLVGEQTVV RSFPIASITK
960 970 980 990 1000
EKKITMQNQL QQSVQEGLQI TSASFQLIKV AFDEEVSPEV VDIFKKQLMK
1010 1020 1030 1040 1050
FRYPQSIFST FAFAAGQTTP QIILPKQKEK NTSFRTFSKT IVKGAKKAGK
1060 1070 1080 1090 1100
MTIGRQYLLK KRTGTIVEER VNRPGWNEED DISVSDDSEL PTSTTLKASE
1110 1120 1130 1140 1150
KSTMEQLVEK ACFRDYQRLG LGTISGNSSR SKPEYFRVTA SNRLYSLCRS
1160 1170 1180 1190 1200
YPGLLVIPQA VQDSSLPRVA RCYRHNRLPV VCWKNSRSGT LLLRSGGFHG
1210 1220 1230 1240 1250
KGVVGLFKSQ NSPQAVSTSS LESSSSIEQE KYLQALLTAV IVHQKLRGSS
1260 1270 1280 1290 1300
TLTVRPALAL SPVHGYRDKS FTQSNPKSSA KEPVHNQGVW ASLRSSTRLI
1310 1320 1330 1340 1350
SSPTSFIDVG ARLAGKDHSA SFSNSTYLQN QLLKRQAALY IFGEKSQLRS
1360 1370 1380 1390 1400
SKVEFAFNCE FVPVEFHEIR QVKASFKKLM RACIPSTIPT DSEVTFLKAL
1410 1420 1430 1440 1450
GDSEWFPQLH RIMQLAVVVS EVLENGSSVW VCLEEGWDIT TQVTSLAQLL
1460 1470 1480 1490 1500
SDPFYRTIAG FRTLVEKEWL SFGHKFSQRS SLALNSQGGG FAPIFLQFLD
1510 1520 1530 1540 1550
CVHQVHNQYP TEFEFNLYYL KFLAFHYVSN RFKTFLLDSD YERLEHGTLF
1560 1570 1580 1590 1600
DDKGDKHAKK GVCIWECIDK MHTRSPIFFN YLYSPVEVEA LKPNVNVSSL
1610 1620 1630 1640 1650
KKWDYYTEET LSAGPSYDWM MLTPKHFPYE ESDVAGGAGP QSQRKTVWPC
1660 1670 1680 1690 1700
YDDVTCSQPD ALTRLFSEIE KLEHKLNQTP ERWHQLWEKV TTDLKEEPRT
1710 1720 1730 1740 1750
AHSLRHSAGS PGIASTNVPS YQKRPALHPL HRGLGEDQST TTAPSNGVEH
1760 1770 1780 1790 1800
RAATLYSQYT SKNDENRSFE GTLYKRGALL KGWKPRWFVL DVTKHQLRYY
1810 1820 1830 1840 1850
DSGEDTSCKG HIDLAEVEMV IPAGPSMGAP KYTSDKAFFD LKTSKRVYNF
1860 1870
CAQDGQSAQQ WMDRIQSCIS DA
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q0D4 | E9Q0D4_MOUSE | Myotubularin-related protein 13 | Sbf2 | 1,826 | Annotation score: | ||
F6QZ02 | F6QZ02_MOUSE | Myotubularin-related protein 13 | Sbf2 | 198 | Annotation score: | ||
F7BBM8 | F7BBM8_MOUSE | Myotubularin-related protein 13 | Sbf2 | 245 | Annotation score: | ||
F6ZDC5 | F6ZDC5_MOUSE | Myotubularin-related protein 13 | Sbf2 | 339 | Annotation score: | ||
E9Q372 | E9Q372_MOUSE | Myotubularin-related protein 13 | Sbf2 | 408 | Annotation score: | ||
F6QG63 | F6QG63_MOUSE | Myotubularin-related protein 13 | Sbf2 | 177 | Annotation score: | ||
E9Q4I8 | E9Q4I8_MOUSE | Myotubularin-related protein 13 | Sbf2 | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 300 | I → F in BAC39860 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1496 | L → F in AAH15069 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060070 | 1263 – 1287 | Missing in isoform 2. CuratedAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC122921 Genomic DNA No translation available. AC124472 Genomic DNA No translation available. AC154911 Genomic DNA No translation available. AK029568 mRNA Translation: BAC26517.1 AK080952 mRNA Translation: BAC38090.1 AK087396 mRNA Translation: BAC39860.1 BC015069 mRNA Translation: AAH15069.1 |
CCDSi | CCDS52361.1 [E9PXF8-1] |
RefSeqi | NP_796298.2, NM_177324.2 [E9PXF8-1] XP_006507989.1, XM_006507926.1 [E9PXF8-2] |
Genome annotation databases
Ensembli | ENSMUST00000033058; ENSMUSP00000033058; ENSMUSG00000038371 [E9PXF8-1] ENSMUST00000164759; ENSMUSP00000132072; ENSMUSG00000038371 [E9PXF8-2] |
GeneIDi | 319934 |
KEGGi | mmu:319934 |
UCSCi | uc009jfe.2, mouse [E9PXF8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC122921 Genomic DNA No translation available. AC124472 Genomic DNA No translation available. AC154911 Genomic DNA No translation available. AK029568 mRNA Translation: BAC26517.1 AK080952 mRNA Translation: BAC38090.1 AK087396 mRNA Translation: BAC39860.1 BC015069 mRNA Translation: AAH15069.1 |
CCDSi | CCDS52361.1 [E9PXF8-1] |
RefSeqi | NP_796298.2, NM_177324.2 [E9PXF8-1] XP_006507989.1, XM_006507926.1 [E9PXF8-2] |
3D structure databases
AlphaFoldDBi | E9PXF8 |
SMRi | E9PXF8 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | E9PXF8, 1 interactor |
STRINGi | 10090.ENSMUSP00000033058 |
PTM databases
iPTMneti | E9PXF8 |
PhosphoSitePlusi | E9PXF8 |
SwissPalmi | E9PXF8 |
Proteomic databases
jPOSTi | E9PXF8 |
MaxQBi | E9PXF8 |
PaxDbi | E9PXF8 |
PeptideAtlasi | E9PXF8 |
ProteomicsDBi | 346740 347539 [E9PXF8-1] |
Protocols and materials databases
Antibodypediai | 24321, 42 antibodies from 11 providers |
DNASUi | 319934 |
Genome annotation databases
Ensembli | ENSMUST00000033058; ENSMUSP00000033058; ENSMUSG00000038371 [E9PXF8-1] ENSMUST00000164759; ENSMUSP00000132072; ENSMUSG00000038371 [E9PXF8-2] |
GeneIDi | 319934 |
KEGGi | mmu:319934 |
UCSCi | uc009jfe.2, mouse [E9PXF8-1] |
Organism-specific databases
CTDi | 81846 |
MGIi | MGI:1921831, Sbf2 |
VEuPathDBi | HostDB:ENSMUSG00000038371 |
Phylogenomic databases
eggNOGi | KOG1090, Eukaryota KOG4471, Eukaryota |
GeneTreei | ENSGT00940000155385 |
HOGENOMi | CLU_002298_1_1_1 |
InParanoidi | E9PXF8 |
OMAi | QQFWEDA |
OrthoDBi | 45015at2759 |
PhylomeDBi | E9PXF8 |
TreeFami | TF318583 |
Enzyme and pathway databases
Reactomei | R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
BioGRID-ORCSi | 319934, 0 hits in 60 CRISPR screens |
ChiTaRSi | Sbf2, mouse |
PROi | PR:E9PXF8 |
RNActi | E9PXF8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038371, Expressed in sciatic nerve and 311 other tissues |
Genevisiblei | E9PXF8, MM |
Family and domain databases
CDDi | cd13339, PH-GRAM_MTMR13, 1 hit |
Gene3Di | 2.30.29.30, 2 hits 3.40.50.11500, 1 hit |
InterProi | View protein in InterPro IPR001194, cDENN_dom IPR005112, dDENN_dom IPR043153, DENN_C IPR004182, GRAM IPR030567, MTMR13 IPR037823, MTMR13_PH-GRAM IPR010569, Myotubularin-like_Pase_dom IPR030564, Myotubularin_fam IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR029021, Prot-tyrosine_phosphatase-like IPR022096, SBF1/SBF2 IPR037516, Tripartite_DENN IPR005113, uDENN_dom |
PANTHERi | PTHR10807, PTHR10807, 1 hit PTHR10807:SF4, PTHR10807:SF4, 1 hit |
Pfami | View protein in Pfam PF02141, DENN, 1 hit PF02893, GRAM, 1 hit PF06602, Myotub-related, 1 hit PF00169, PH, 1 hit PF12335, SBF2, 1 hit PF03456, uDENN, 1 hit |
SMARTi | View protein in SMART SM00801, dDENN, 1 hit SM00799, DENN, 1 hit SM00568, GRAM, 1 hit SM00233, PH, 1 hit SM00800, uDENN, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS50211, DENN, 1 hit PS50003, PH_DOMAIN, 1 hit PS51339, PPASE_MYOTUBULARIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MTMRD_MOUSE | |
Accessioni | E9PXF8Primary (citable) accession number: E9PXF8 Secondary accession number(s): E9Q305 Q91VH0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 13, 2019 |
Last sequence update: | April 5, 2011 | |
Last modified: | May 25, 2022 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families