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Protein

eIF-2-alpha kinase activator GCN1

Gene

Gcn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a positive activator of the GCN2 protein kinase activity in response to amino acid starvation (PubMed:15937339). Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity (By similarity). Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:24333428).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processStress response, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
eIF-2-alpha kinase activator GCN1Curated
Alternative name(s):
GCN1 eIF-2-alpha kinase activator homologBy similarity
GCN1-like protein 1Imported
General control of amino-acid synthesis 1-like protein 1Imported
Translational activator GCN1Curated
Gene namesi
Name:Gcn1By similarity
Synonyms:Gcn1l1Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2444248 Gcn1l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004354242 – 2671eIF-2-alpha kinase activator GCN1Add BLAST2670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei729PhosphoserineBy similarity1
Modified residuei786PhosphoserineBy similarity1
Modified residuei2276PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiE9PVA8
PaxDbiE9PVA8
PeptideAtlasiE9PVA8
PRIDEiE9PVA8

PTM databases

iPTMnetiE9PVA8
PhosphoSitePlusiE9PVA8

Expressioni

Tissue specificityi

Expressed in the hypothalamus, cortex and hippocampus (PubMed:15937339).1 Publication

Gene expression databases

BgeeiENSMUSG00000041638 Expressed in 157 organ(s), highest expression level in secondary oocyte
GenevisibleiE9PVA8 MM

Interactioni

Subunit structurei

Interacts with EIF2AK4/GCN2; this interaction stimulates the EIF2AK4/GCN2 kinase activity and is impaired by IMPACT upon a variety of stress conditions, such as amino acid depletion, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation (PubMed:24333428). Interacts with IMPACT; this prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity (PubMed:15937339, PubMed:22404850).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiE9PVA8, 3 interactors
STRINGi10090.ENSMUSP00000069432

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati140 – 178HEAT 1Sequence analysisAdd BLAST39
Repeati257 – 293HEAT 2Sequence analysisAdd BLAST37
Repeati294 – 331HEAT 3Sequence analysisAdd BLAST38
Repeati385 – 423HEAT 4Sequence analysisAdd BLAST39
Repeati425 – 459HEAT 5Sequence analysisAdd BLAST35
Repeati460 – 500HEAT 6Sequence analysisAdd BLAST41
Repeati560 – 597HEAT 7Sequence analysisAdd BLAST38
Repeati599 – 636HEAT 8Sequence analysisAdd BLAST38
Repeati700 – 732HEAT 9Sequence analysisAdd BLAST33
Repeati733 – 772HEAT 10Sequence analysisAdd BLAST40
Repeati879 – 918HEAT 11Sequence analysisAdd BLAST40
Repeati979 – 1016HEAT 12Sequence analysisAdd BLAST38
Repeati1035 – 1072HEAT 13Sequence analysisAdd BLAST38
Repeati1078 – 1115HEAT 14Sequence analysisAdd BLAST38
Repeati1155 – 1192HEAT 15Sequence analysisAdd BLAST38
Repeati1210 – 1250HEAT 16Sequence analysisAdd BLAST41
Repeati1251 – 1289HEAT 17Sequence analysisAdd BLAST39
Repeati1290 – 1332HEAT 18Sequence analysisAdd BLAST43
Repeati1335 – 1372HEAT 19Sequence analysisAdd BLAST38
Repeati1374 – 1410HEAT 20Sequence analysisAdd BLAST37
Repeati1413 – 1451HEAT 21Sequence analysisAdd BLAST39
Repeati1455 – 1492HEAT 22Sequence analysisAdd BLAST38
Repeati1493 – 1530HEAT 23Sequence analysisAdd BLAST38
Repeati1534 – 1571HEAT 24Sequence analysisAdd BLAST38
Repeati1573 – 1609HEAT 25Sequence analysisAdd BLAST37
Repeati1611 – 1648HEAT 26Sequence analysisAdd BLAST38
Repeati1653 – 1690HEAT 27Sequence analysisAdd BLAST38
Repeati1692 – 1729HEAT 28Sequence analysisAdd BLAST38
Repeati1731 – 1769HEAT 29Sequence analysisAdd BLAST39
Repeati1773 – 1810HEAT 30Sequence analysisAdd BLAST38
Repeati1812 – 1848HEAT 31Sequence analysisAdd BLAST37
Repeati1921 – 1958HEAT 32Sequence analysisAdd BLAST38
Repeati1959 – 1996HEAT 33Sequence analysisAdd BLAST38
Repeati2001 – 2038HEAT 34Sequence analysisAdd BLAST38
Repeati2039 – 2074HEAT 35Sequence analysisAdd BLAST36
Repeati2076 – 2108HEAT 36Sequence analysisAdd BLAST33
Repeati2111 – 2146HEAT 37Sequence analysisAdd BLAST36
Repeati2147 – 2184HEAT 38Sequence analysisAdd BLAST38
Repeati2188 – 2225HEAT 39Sequence analysisAdd BLAST38
Repeati2259 – 2296HEAT 40Sequence analysisAdd BLAST38
Repeati2301 – 2338HEAT 41Sequence analysisAdd BLAST38
Repeati2339 – 2380HEAT 42Sequence analysisAdd BLAST42
Repeati2382 – 2417HEAT 43Sequence analysisAdd BLAST36
Repeati2422 – 2459HEAT 44Sequence analysisAdd BLAST38
Repeati2546 – 2583HEAT 45Sequence analysisAdd BLAST38
Repeati2588 – 2625HEAT 46Sequence analysisAdd BLAST38
Repeati2627 – 2661HEAT 47Sequence analysisAdd BLAST35

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili804 – 865Sequence analysisAdd BLAST62

Sequence similaritiesi

Belongs to the GCN1 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1242 Eukaryota
ENOG410XPSD LUCA
GeneTreeiENSGT00390000011675
HOGENOMiHOG000213714
HOVERGENiHBG081550
InParanoidiE9PVA8
OMAiDAKQGIC
OrthoDBiEOG091G008Q
TreeFamiTF105398

Family and domain databases

Gene3Di1.25.10.10, 5 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR033173 Gcn1
IPR021133 HEAT_type_2
IPR034085 TOG
PANTHERiPTHR23346:SF7 PTHR23346:SF7, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 5 hits
SM01349 TOG, 1 hit
SUPFAMiSSF48371 SSF48371, 4 hits
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E9PVA8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAADTQVSET LKRFAVKVTT ASVKERREIL SELGRCIAGK DLPEGAVKGL
60 70 80 90 100
CKLFCLTLHR YRDAASRRAL QAAIQQLAEA QPEATAKNLL HSLQSSGVGS
110 120 130 140 150
KACVPSKSSG SAALLALTWT CLLVRIVFPL KAKRQGDIWN KLVEVQCLLL
160 170 180 190 200
LEVLGGSHKH AVDGAVKKLT KLWKENPGLV EQYFSAILSL EPSQNYAAML
210 220 230 240 250
GLLVQFCTNH KEMDAVSQHK STLLEFYVKN ILMSKAKPPK YLLDNCAPLL
260 270 280 290 300
RFMSHSEFKD LILPTIQKSL LRSPENVIET ISSLLASVTL DLSQYALDIV
310 320 330 340 350
KGLANQLKSN SPRLMDEAVL ALRNLARQCS DSSATEALTK HLFAILGGSE
360 370 380 390 400
GKLTIIAQKM SVLSGIGSLS HHVVSGPSGQ VLNGCVAELF IPFLQQEVHE
410 420 430 440 450
GTLVHAVSIL ALWCNRFTTE VPKKLTDWFK KVFSLKTSTS AVRHAYLQCM
460 470 480 490 500
LASFRGDTLL QALDFLPLLM QTVEKAASQG TQVPTVTEGV AAALLLSKLS
510 520 530 540 550
VADAQAEAKL SGFWQLVVDE KRQTFTSEKF LLLASEDALC TVLRLTERLF
560 570 580 590 600
LDHPHRLTNS KVQQYYRVLV AVLLSRTWHV RRQAQQTVRK LLSSLGGVKL
610 620 630 640 650
ANGLLDELKT VLNSHKVLPL EALVTDAGEV TEMGKTYVPP RVLQEALCVI
660 670 680 690 700
SGVPGLKGDI PSTEQLAQEM LIISHHPSLV AVQSGLWPAL LTRMKIDPDA
710 720 730 740 750
FITRHLDQII PRITTQSPLN QSSMNAMGSL SVLSPDRVLP QLISTITASV
760 770 780 790 800
QNPALCLVTR EEFSIMQTPA GELFDKSIIQ SAQQDSIKKA NMKRENKAYS
810 820 830 840 850
FKEQIIEMEL KEEIKKKKGI KEEVQLTSKQ KEMLQAQMDK EAQIRRRLQE
860 870 880 890 900
LDGELEAALG LLDAIMARNP CGLIQYIPVL VDAFLPLLKS PLAAPRVKGP
910 920 930 940 950
FLSLAACVMP PRLKTLGTLV SHVTLRLLKP ECALDKSWCQ EELPVAVRRA
960 970 980 990 1000
VSLLHTHTIP SRVGKGEPDA APLSAPAFSL VFPMLKMVLT EMPYHSEEEE
1010 1020 1030 1040 1050
EQMAQILQIL TVHAQLRASP DTPPERVDEN GPELLPRVAM LRLLTWVIGT
1060 1070 1080 1090 1100
GSPRLQVLAS DTLTALCASS SGEDGCAFAE QEEVDVLLAA LQSPCASVRE
1110 1120 1130 1140 1150
TALRGLMELR LVLPSPDTDE KSGLSLLRRL WVIKFDKEDE IRKLAERLWS
1160 1170 1180 1190 1200
TMGLDLQSDL CSLLIDDVIY HEAAVRQAGA EALSQAVARY QRQAAEVMGR
1210 1220 1230 1240 1250
LMEIYQEKLY RPPPVLDALG RVISESPPDQ WEARCGLALA LNKLSQYLDS
1260 1270 1280 1290 1300
SQVKPLFQFF VPDALNDRNP DVRKCMLDAA LATLNAHGKE NVNSLLPVFE
1310 1320 1330 1340 1350
EFLKDAPNDA SYDAVRQSVV VLMGSLAKHL DKSDPKVKPI VAKLIAALST
1360 1370 1380 1390 1400
PSQQVQESVA SCLPPLVPAV KEDAGGMIQR LMQQLLESDK YAERKGAAYG
1410 1420 1430 1440 1450
LAGLVKGLGI LSLKQQEMMA ALTDAIQDKK NFRRREGALF AFEMLCTMLG
1460 1470 1480 1490 1500
KLFEPYVVHV LPHLLLCFGD GNQYVREAAD DCAKAVMSNL SAHGVKLVLP
1510 1520 1530 1540 1550
SLLAALEEES WRTKAGSVEL LGAMAYCAPK QLSSCLPNIV PKLTEVLTDS
1560 1570 1580 1590 1600
HVKVQKAGQQ ALRQIGSVIR NPEILAIAPV LLDALTDPSR KTQKCLQTLL
1610 1620 1630 1640 1650
DTKFVHFIDA PSLALIMPIV QRAFQDRSTD TRKMAAQIIG NMYSLTDQKD
1660 1670 1680 1690 1700
LAPYLPSVTP GLKASLLDPV PEVRTVSAKA LGAMVKGMGE SCFEDLLPWL
1710 1720 1730 1740 1750
METLTYEQSS VDRSGAAQGL AEVMAGLGVE KLEKLMPEIV ATASKVDIAP
1760 1770 1780 1790 1800
HVRDGYIMMF NYLPITFGDK FTPYVGPIIP CILKALADEN EFVRDTALRA
1810 1820 1830 1840 1850
GQRVISMYAE TAIALLLPQL EQGLFDDLWR IRFSSVQLLG DLLFHISGVT
1860 1870 1880 1890 1900
GKMTTETASE DDNFGTAQSN KAIITALGVD RRNRVLAGLY MGRSDTQLVV
1910 1920 1930 1940 1950
RQASLHVWKI VVSNTPRTLR EILPTLFGLL LGFLASTCAD KRTIAARTLG
1960 1970 1980 1990 2000
DLVRKLGEKI LPEIIPILEE GLRSQKSDER QGVCIGLSEI MKSTSRDAVL
2010 2020 2030 2040 2050
FFSESLVPTA RKALCDPLEE VREAAAKTFE QLHSTIGHQA LEDILPFLLK
2060 2070 2080 2090 2100
QLDDEEVSEF ALDGLKQVMA VKSRVVLPYL VPKLTTPPVN TRVLAFLSSV
2110 2120 2130 2140 2150
AGDALTRHLG VILPAVMLAL KEKLGTPDEQ LEMANCQAVI LSVEDDTGHR
2160 2170 2180 2190 2200
IIIEDLLEAT RSPEVGMRQA AAIILNMYCS RSKADYSSHL RSLVSGLIRL
2210 2220 2230 2240 2250
FNDSSPVVLE ESWDALNAIT KKLDAGNQLA LIEELHKEIR FIGNECKGEH
2260 2270 2280 2290 2300
VPGFCLPKRG VTSILPVLRE GVLTGSPEQK EEAAKGLGLV IRLTSADALR
2310 2320 2330 2340 2350
PSVVSITGPL IRILGDRFNW TVKAALLETL SLLLGKVGIA LKPFLPQLQT
2360 2370 2380 2390 2400
TFTKALQDSN RGVRLKAADA LGKLISIHVK VDPLFTELLN GIRAVEDPGI
2410 2420 2430 2440 2450
RDTMLQALRF VIQGAGSKVD AAIRKNLVSL LLSMLGHDED NTRISTAGCL
2460 2470 2480 2490 2500
GELCAFLTDE ELNTVLQQCL LADVSGIDWM VRHGRSLALS VAVNVAPSRL
2510 2520 2530 2540 2550
CAGRYSNEVQ DMILSNAVAD RIPIAMSGIR GMGFLMKYHI ETGSGQLPPR
2560 2570 2580 2590 2600
LSSLLIKCLQ NPCSDIRLVA EKMIWWANKE PRPPLEPQTI KPILKALLDN
2610 2620 2630 2640 2650
TKDKNTVVRA YSDQAIVNLL KMRRGEELLQ SLSKILDVAS LEALNECSRR
2660 2670
SLRKLACQAD SVEQVDDTIL T
Length:2,671
Mass (Da):293,021
Last modified:April 5, 2011 - v1
Checksum:i77B317C0797DC5A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti466L → V in AAI50736 (PubMed:15489334).Curated1
Sequence conflicti537D → E in AAI50736 (PubMed:15489334).Curated1
Sequence conflicti1477E → K in AAI50736 (PubMed:15489334).Curated1
Sequence conflicti2544S → G in AAI50736 (PubMed:15489334).Curated1
Sequence conflicti2583P → H in AAI50736 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC159539 Genomic DNA No translation available.
BC150735 mRNA Translation: AAI50736.1
CCDSiCCDS19595.1
RefSeqiNP_766307.2, NM_172719.2
UniGeneiMm.153014

Genome annotation databases

EnsembliENSMUST00000064454; ENSMUSP00000069432; ENSMUSG00000041638
GeneIDi231659
KEGGimmu:231659
UCSCiuc008zeg.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC159539 Genomic DNA No translation available.
BC150735 mRNA Translation: AAI50736.1
CCDSiCCDS19595.1
RefSeqiNP_766307.2, NM_172719.2
UniGeneiMm.153014

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiE9PVA8, 3 interactors
STRINGi10090.ENSMUSP00000069432

PTM databases

iPTMnetiE9PVA8
PhosphoSitePlusiE9PVA8

Proteomic databases

MaxQBiE9PVA8
PaxDbiE9PVA8
PeptideAtlasiE9PVA8
PRIDEiE9PVA8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064454; ENSMUSP00000069432; ENSMUSG00000041638
GeneIDi231659
KEGGimmu:231659
UCSCiuc008zeg.1 mouse

Organism-specific databases

CTDi231659
MGIiMGI:2444248 Gcn1l1

Phylogenomic databases

eggNOGiKOG1242 Eukaryota
ENOG410XPSD LUCA
GeneTreeiENSGT00390000011675
HOGENOMiHOG000213714
HOVERGENiHBG081550
InParanoidiE9PVA8
OMAiDAKQGIC
OrthoDBiEOG091G008Q
TreeFamiTF105398

Miscellaneous databases

ChiTaRSiGcn1l1 mouse
PROiPR:E9PVA8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041638 Expressed in 157 organ(s), highest expression level in secondary oocyte
GenevisibleiE9PVA8 MM

Family and domain databases

Gene3Di1.25.10.10, 5 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR033173 Gcn1
IPR021133 HEAT_type_2
IPR034085 TOG
PANTHERiPTHR23346:SF7 PTHR23346:SF7, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 5 hits
SM01349 TOG, 1 hit
SUPFAMiSSF48371 SSF48371, 4 hits
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGCN1_MOUSE
AccessioniPrimary (citable) accession number: E9PVA8
Secondary accession number(s): B2RWW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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