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UniProtKB - E9PT87 (MYLK3_RAT)
Protein
Myosin light chain kinase 3
Gene
Mylk3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Calmodulin-dependent kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes. Increases cardiomyocyte contractility.
2 PublicationsCatalytic activityi
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 511 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 603 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 488 – 496 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin-dependent protein kinase activity Source: BHF-UCL
- myosin light chain kinase activity Source: BHF-UCL
GO - Biological processi
- cardiac myofibril assembly Source: BHF-UCL
- cellular response to interleukin-1 Source: RGD
- positive regulation of sarcomere organization Source: BHF-UCL
- protein phosphorylation Source: BHF-UCL
- regulation of vascular permeability involved in acute inflammatory response Source: RGD
- sarcomere organization Source: BHF-UCL
- sarcomerogenesis Source: BHF-UCL
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Myosin light chain kinase 3 (EC:2.7.11.18)Alternative name(s): Cardiac-MyBP-C-associated Ca/CaM kinase Short name: Cardiac-MLCK |
Gene namesi | Name:Mylk3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305801, Mylk3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- actin cytoskeleton Source: Ensembl
Cytosol
- cytosol Source: BHF-UCL
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000419665 | 1 – 786 | Myosin light chain kinase 3Add BLAST | 786 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 155 | PhosphoserineCombined sources | 1 | |
Modified residuei | 341 | PhosphoserineCombined sources | 1 | |
Modified residuei | 422 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated on serine residues.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | E9PT87 |
PaxDbi | E9PT87 |
PeptideAtlasi | E9PT87 |
PRIDEi | E9PT87 |
PTM databases
iPTMneti | E9PT87 |
PhosphoSitePlusi | E9PT87 |
Expressioni
Tissue specificityi
Expressed in cardiomyocytes (at protein level). Up-regulated in heart after experimental myocardial infarction at the mRNA level.2 Publications
Developmental stagei
Up-regulated in the heart from 1 week after birth through adulthood.1 Publication
Inductioni
by NRG1 (at protein level).1 Publication
Gene expression databases
Bgeei | ENSRNOG00000017546, Expressed in heart and 19 other tissues |
Genevisiblei | E9PT87, RN |
Interactioni
Protein-protein interaction databases
BioGRIDi | 253688, 1 interactor |
STRINGi | 10116.ENSRNOP00000023657 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 482 – 737 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 233 – 258 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 279 – 315 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 333 – 443 | DisorderedSequence analysisAdd BLAST | 111 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 279 – 296 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000160007 |
HOGENOMi | CLU_000288_90_0_1 |
InParanoidi | E9PT87 |
OMAi | DPWPLEE |
OrthoDBi | 218222at2759 |
TreeFami | TF314166 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
E9PT87-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGVSEEDPE GLGPQGLPAL GGACLVTVDK KLNVLTEKVD RLLHFQEDVT
60 70 80 90 100
EKLQCVCQGM DHLEQGLHRL EASQELGLAG PGSTSPAAAQ AAWPEVLELV
110 120 130 140 150
RAVRQEGAQH GARLEALFKM VVAVDRAITL VGSTIQNSKV DDFILQGTVP
160 170 180 190 200
WRKGSLADGP EENKEQAEVA GVKPKHVLNT GSVQAATSRA LWEESQKQDT
210 220 230 240 250
PVGTVEGLPL IIDTSLKGAD LTQAGASLRQ GVEALDPGQE PPPTEAESRL
260 270 280 290 300
PALASEDTGT TLELSVAIDR ISEVLTSLRM SQSAGEGTSS SKPDCSEPGP
310 320 330 340 350
QPLGPLTTDS DIHSDEGLPR ISVRMREMTT PEELFETQGG SPIGSAEAPG
360 370 380 390 400
PGTVLEDQIP KGARPFPPLP KRSCNNGGAS AEEATGPGAE PIRGPSLVTR
410 420 430 440 450
DWRDEPVGTT DLQQGRDPGA VSPEPGKDHA AQGPGRTEAG RRVSSAAEAA
460 470 480 490 500
IVVLDDSAAP PAPFEHRVVS IKDTLISTSY TVSQHEVLGG GRFGQVHRCT
510 520 530 540 550
ERSTGLALAA KIIKVKNIKD REDVKNEINI MNQLSHVNLI QLYDAFESKN
560 570 580 590 600
SFTLIMEYVD GGELFDRITD EKYHLTELDV VLFTRQICEG VHYLHQHYIL
610 620 630 640 650
HLDLKPENIL CVSQTGHQIK IIDFGLARRY KPREKLKVNF GTPEFLAPEV
660 670 680 690 700
VNYEFVSFPT DMWSVGVITY MLLSGLSPFL GETDAETMNF IVNCSWDFDA
710 720 730 740 750
DTFKGLSEEA KDFVSRLLVK EKSCRMSATQ CLKHEWLNHL IAKASGSNVR
760 770 780
LRSQLLLQKY MAQRKWKKHF HVVTAVNRLR KFPTCP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I5ZYT9 | A0A8I5ZYT9_RAT | Myosin light chain kinase 3 | Mylk3 | 653 | Annotation score: | ||
A0A8I6GIK4 | A0A8I6GIK4_RAT | Myosin light chain kinase 3 | Mylk3 | 737 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 244 | T → A in ABW96144 (PubMed:20615916).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU236721 mRNA Translation: ABW96144.1 |
RefSeqi | NP_001104280.1, NM_001110810.1 |
Genome annotation databases
Ensembli | ENSRNOT00000023657; ENSRNOP00000023657; ENSRNOG00000017546 |
GeneIDi | 291926 |
KEGGi | rno:291926 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU236721 mRNA Translation: ABW96144.1 |
RefSeqi | NP_001104280.1, NM_001110810.1 |
3D structure databases
AlphaFoldDBi | E9PT87 |
SMRi | E9PT87 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 253688, 1 interactor |
STRINGi | 10116.ENSRNOP00000023657 |
PTM databases
iPTMneti | E9PT87 |
PhosphoSitePlusi | E9PT87 |
Proteomic databases
jPOSTi | E9PT87 |
PaxDbi | E9PT87 |
PeptideAtlasi | E9PT87 |
PRIDEi | E9PT87 |
Genome annotation databases
Ensembli | ENSRNOT00000023657; ENSRNOP00000023657; ENSRNOG00000017546 |
GeneIDi | 291926 |
KEGGi | rno:291926 |
Organism-specific databases
CTDi | 91807 |
RGDi | 1305801, Mylk3 |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000160007 |
HOGENOMi | CLU_000288_90_0_1 |
InParanoidi | E9PT87 |
OMAi | DPWPLEE |
OrthoDBi | 218222at2759 |
TreeFami | TF314166 |
Miscellaneous databases
PROi | PR:E9PT87 |
Gene expression databases
Bgeei | ENSRNOG00000017546, Expressed in heart and 19 other tissues |
Genevisiblei | E9PT87, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MYLK3_RAT | |
Accessioni | E9PT87Primary (citable) accession number: E9PT87 Secondary accession number(s): A8WE96 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | April 5, 2011 | |
Last modified: | May 25, 2022 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families