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UniProtKB - E9CXX8 (MNS1B_COCPS)
Protein
Mannosyl-oligosaccharide alpha-1,2-mannosidase
Gene
CPSG_02648
Organism
Coccidioides posadasii (strain RMSCC 757 / Silveira) (Valley fever fungus)
Status
Functioni
Alpha-mannosidase involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
2 PublicationsCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)1 PublicationEC:3.2.1.1131 Publication
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)1 PublicationEC:3.2.1.1131 Publication
Cofactori
Ca2+By similarity, Mg2+By similarityNote: Ca2+. Can also use Mg2+, but with lower efficiency.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 380 | Proton donorBy similarity | 1 | |
Metal bindingi | 507 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Protein family/group databases
CLAEi | MSD47A_COCPO |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide alpha-1,2-mannosidaseCurated (EC:3.2.1.1131 Publication)Alternative name(s): Class I alpha-mannosidase1 Publication Man(9)-alpha-mannosidaseCurated |
Gene namesi | ORF Names:CPSG_02648 |
Organismi | Coccidioides posadasii (strain RMSCC 757 / Silveira) (Valley fever fungus) |
Taxonomic identifieri | 443226 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Onygenales incertae sedis › Coccidioides › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:CPSG_02648 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- membrane Source: InterPro
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000432784 | 23 – 519 | Mannosyl-oligosaccharide alpha-1,2-mannosidaseSequence analysisAdd BLAST | 497 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 187 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 337 ↔ 366 | By similarity | ||
Glycosylationi | 443 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 443226.E9CXX8 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
HOGENOMi | CLU_003818_0_2_1 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
E9CXX8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGSPVLAVC AAALTLIPSV VALPMIDKDL PSSISQSSDK TSQERAEAVK
60 70 80 90 100
AAFRFAWEGY LEHAFPNDEL HPVSNTPGNS RNGWGASAVD ALSTAIIMDM
110 120 130 140 150
PDVVEKILDH ISNIDYSQTD TMCSLFETTI RYLGGMISAY DLLKGPGSHL
160 170 180 190 200
VSDPAKVDVL LAQSLKLADV LKFAFDTKTG IPANELNITD KSTDGSTTNG
210 220 230 240 250
LATTGTLVLE WTRLSDITGD PEYGRLAQKG ESYLLNPQPS SSEPFPGLVG
260 270 280 290 300
RTIDIETGLF RDDYVSWGGG SDSFYEYLIK MYVYDKGRFG KYKDRWVTAA
310 320 330 340 350
ESTIEHLKSS PSTRKDLTFV ATYSGGRLGL NSGHLTCFDG GNFLLGGQIL
360 370 380 390 400
NRDDFTKFGL ELVEGCYATY AATATKIGPE GFGWDATKVP EAQAEFYKEA
410 420 430 440 450
GFYITTSYYN LRPEVIESIY YAYRMTKDPK YQEWAWDAFV AINATTRTST
460 470 480 490 500
GFTAIGDVNT PDGGRKYDNQ ESFLFAEVMK YSYLIHSPEA DWQVAGPGGT
510
NAYVFNTEAH PVKVFSRGC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ233502 mRNA Translation: ABB36773.3 GL636488 Genomic DNA Translation: EFW20805.1 |
Genome annotation databases
EnsemblFungii | EFW20805; EFW20805; CPSG_02648 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ233502 mRNA Translation: ABB36773.3 GL636488 Genomic DNA Translation: EFW20805.1 |
3D structure databases
SMRi | E9CXX8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 443226.E9CXX8 |
Protein family/group databases
CLAEi | MSD47A_COCPO |
Genome annotation databases
EnsemblFungii | EFW20805; EFW20805; CPSG_02648 |
Organism-specific databases
VEuPathDBi | FungiDB:CPSG_02648 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
HOGENOMi | CLU_003818_0_2_1 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MNS1B_COCPS | |
Accessioni | E9CXX8Primary (citable) accession number: E9CXX8 Secondary accession number(s): A0A059NZV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2015 |
Last sequence update: | April 5, 2011 | |
Last modified: | February 23, 2022 | |
This is version 39 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families