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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Blochmannia vafer (strain BVAF)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotationSAAS annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II).UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II), the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional protein GlmU (glmU)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28UDP-GlcNAcUniRule annotation1
Binding sitei84UDP-GlcNAcUniRule annotation1
Metal bindingi113MagnesiumUniRule annotation1
Binding sitei148UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei163UDP-GlcNAcUniRule annotation1
Binding sitei179UDP-GlcNAcUniRule annotation1
Metal bindingi237MagnesiumUniRule annotation1
Binding sitei237UDP-GlcNAcUniRule annotation1
Binding sitei343UDP-GlcNAcUniRule annotation1
Binding sitei361UDP-GlcNAcUniRule annotation1
Active sitei373Proton acceptorUniRule annotation1
Binding sitei376UDP-GlcNAcUniRule annotation1
Binding sitei387UDP-GlcNAcUniRule annotation1
Binding sitei390Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei415Acetyl-CoAUniRule annotation1
Binding sitei433Acetyl-CoA; via amide nitrogenUniRule annotation1
Binding sitei450Acetyl-CoAUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCBLO859654:G1GQW-10-MONOMER
UniPathwayiUPA00113; UER00532
UPA00113; UER00533
UPA00973

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Gene namesi
Name:glmUUniRule annotationImported
Ordered Locus Names:BVAF_010Imported
OrganismiBlochmannia vafer (strain BVAF)Imported
Taxonomic identifieri859654 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeant endosymbiontsCandidatus Blochmannia
Proteomesi
  • UP000007464 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotrimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi859654.BVAF_010

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 142NTP_transf_3InterPro annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 239PyrophosphorylaseUniRule annotationAdd BLAST239
Regioni14 – 17UDP-GlcNAc bindingUniRule annotation4
Regioni89 – 90UDP-GlcNAc bindingUniRule annotation2
Regioni111 – 113UDP-GlcNAc bindingUniRule annotation3
Regioni240 – 260LinkerUniRule annotationAdd BLAST21
Regioni261 – 465N-acetyltransferaseUniRule annotationAdd BLAST205
Regioni396 – 397Acetyl-CoA bindingUniRule annotation2

Sequence similaritiesi

In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CAJ Bacteria
COG1207 LUCA
HOGENOMiHOG000283476
KOiK04042
OMAiIEPQTHL
OrthoDBiPOG091H02I2

Family and domain databases

CDDicd03353 LbH_GlmU_C, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_01631 GlmU, 1 hit
InterProiView protein in InterPro
IPR005882 Bifunctional_GlmU
IPR038009 GlmU_C_LbH
IPR001451 Hexapep
IPR018357 Hexapep_transf_CS
IPR025877 MobA-like_NTP_Trfase
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 3 hits
PF14602 Hexapep_2, 1 hit
PF12804 NTP_transf_3, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01173 glmU, 1 hit
PROSITEiView protein in PROSITE
PS00101 HEXAPEP_TRANSFERASES, 1 hit

Sequencei

Sequence statusi: Complete.

E8Q5V5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCTVWNKNFN VIILAAGYGE RMLSDIPKVM HKIAGKSMLQ HLIDSVCELD
60 70 80 90 100
IQLSIYVVYS CKKDMFVNSI CINDKNISIS WVFQGIPKGT GHAVQKVLPM
110 120 130 140 150
LYEDSEILVL YGDVPLISSR TLKKLLSVKL ESDIALLTAN VNDPTGYGRI
160 170 180 190 200
IRNEFGQVID IKEDSDIVND NHRKITEINS GIFISVSKYI KNWVGIVQNN
210 220 230 240 250
NVKNELYLTD IFKIAYQNKY RINTINPIDE FEIIGINSKL DLIKVERIYQ
260 270 280 290 300
KNQAYNFVKS GVVILDPYRF DLRGTLVCGK DVYIDVNVII EGCVSLGNRV
310 320 330 340 350
KIGAGCILKN VEIGDDVIIC PFSFIENSKI SFASKIGPFS RLRPNTQLGE
360 370 380 390 400
KTYIGNFVEL KNVQLGKKSK VGHLSYLGDA QIGNQVNIGA GTIICNYDGI
410 420 430 440 450
KKHQTYIEDD VFIGSDSQLI APIRIGKSAI IGAGTTVTKN VEEGKTVISR
460
IQQFSISNKK YTDKK
Length:465
Mass (Da):51,940
Last modified:April 5, 2011 - v1
Checksum:i4CC06DD228D9FBC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002189 Genomic DNA Translation: ADV33424.1
RefSeqiWP_013516349.1, NC_014909.2

Genome annotation databases

EnsemblBacteriaiADV33424; ADV33424; BVAF_010
KEGGibva:BVAF_010

Similar proteinsi

Entry informationi

Entry nameiE8Q5V5_BLOVB
AccessioniPrimary (citable) accession number: E8Q5V5
Entry historyiIntegrated into UniProtKB/TrEMBL: April 5, 2011
Last sequence update: April 5, 2011
Last modified: June 20, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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