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Entry version 43 (11 Dec 2019)
Sequence version 1 (05 Apr 2011)
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Protein

Non-reducing end beta-L-arabinofuranosidase

Gene

hypBA1

Organism
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Beta-L-arabinofuranosidase that removes the beta-L-arabinofuranose residue from the non-reducing end of various substrates, including beta-L-arabinofuranosyl-hydroxyproline (Ara-Hyp), Ara-beta-1,2-Ara-beta-Hyp (Ara2-Hyp), Ara-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara3-Hyp), and beta-L-arabinofuranosyl-(1->2)-1-O-methyl-beta-L-arabinofuranose. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 2.0 sec(-1) with beta-Ara2. kcat is 0.013 sec(-1) with Ara-Hyp. kcat is 6.3 sec(-1) with Ara2-Hyp.
  1. KM=0.85 mM for L-arabinofuranose-beta-1,2-L-arabinofuranose disaccharide (beta-Ara2)1 Publication
  2. KM=0.43 mM for Ara-Hyp1 Publication
  3. KM=0.31 mM for Ara2-Hyp1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    BLON565042:G1GTK-233-MONOMER

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    3.2.1.185 851

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...
    CAZyi
    GH127 Glycoside Hydrolase Family 127

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Non-reducing end beta-L-arabinofuranosidase (EC:3.2.1.185)
    Alternative name(s):
    Beta-L-arabinofuranosidase HypBA1
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:hypBA1
    Ordered Locus Names:BLLJ_0211
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri565042 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi322E → A: Almost abolishes enzyme activity. 1 Publication1
    Mutagenesisi338E → A: Insoluble protein with remaining enzyme acvtivity. 1 Publication1
    Mutagenesisi366E → A: Abolishes enzyme activity. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004235551 – 658Non-reducing end beta-L-arabinofuranosidaseAdd BLAST658

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1658
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    E8MGH8

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycosyl hydrolase 127 family.Curated

    Phylogenomic databases

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000115384

    KEGG Orthology (KO)

    More...
    KOi
    K18205

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    LYSGMAD

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012878 Glyco_hydro_127

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF07944 Glyco_hydro_127, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF48208 SSF48208, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    E8MGH8-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MNVTITSPFW KRRRDQIVES VIPYQWGVMN DEIDTTVPDD PAGNQLADSK
    60 70 80 90 100
    SHAVANLKVA AGELDDEFHG MVFQDSDVYK WLEEAAYALA YHPDPELKAL
    110 120 130 140 150
    CDRTVDLIAR AQQSDGYLDT PYQIKSGVWA DRPRFSLIQQ SHEMYVMGHY
    160 170 180 190 200
    IEAAVAYHQV TGNEQALEVA KKMADCLDAN FGPEEGKIHG ADGHPEIELA
    210 220 230 240 250
    LAKLYEETGE KRYLTLSQYL IDVRGQDPQF YAKQLKAMNG DNIFHDLGFY
    260 270 280 290 300
    KPTYFQAAEP VRDQQTADGH AVRVGYLCTG VAHVGRLLGD QGLIDTAKRF
    310 320 330 340 350
    WKNIVTRRMY VTGAIGSTHV GESFTYDYDL PNDTMYGETC ASVAMSMFAQ
    360 370 380 390 400
    QMLDLEPKGE YADVLEKELF NGSIAGISLD GKQYYYVNAL ETTPDGLDNP
    410 420 430 440 450
    DRHHVLSHRV DWFGCACCPA NIARLIASVD RYIYTERDGG KTVLSHQFIA
    460 470 480 490 500
    NTAEFASGLT VEQRSNFPWD GHVEYTVSLP ASATDSSVRF GLRIPGWSRG
    510 520 530 540 550
    SYTLTVNGKP AVGSLEDGFV YLVVNAGDTL EIALELDMSV KFVRANSRVR
    560 570 580 590 600
    SDAGQVAVMR GPLVYCAEQV DNPGDLWNYR LADGVTGADA AVAFQADLLG
    610 620 630 640 650
    GVDTVDLPAV REHADEDDAP LYVDADEPRA GEPATLRLVP YYSWANREIG

    EMRVFQRR
    Length:658
    Mass (Da):73,098
    Last modified:April 5, 2011 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i525D5452021EECB1
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AB619598 Genomic DNA Translation: BAK79118.1
    AP010888 Genomic DNA Translation: BAJ65881.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_013582351.1, NC_015067.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    BAJ65881; BAJ65881; BLLJ_0211

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    blm:BLLJ_0211

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB619598 Genomic DNA Translation: BAK79118.1
    AP010888 Genomic DNA Translation: BAJ65881.1
    RefSeqiWP_013582351.1, NC_015067.1

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3WKWX-ray2.20A1-658[»]
    3WKXX-ray2.00A1-658[»]
    3WREX-ray2.78A1-658[»]
    3WRFX-ray2.25A1-658[»]
    3WRGX-ray2.23A1-658[»]
    SMRiE8MGH8
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein family/group databases

    CAZyiGH127 Glycoside Hydrolase Family 127

    Genome annotation databases

    EnsemblBacteriaiBAJ65881; BAJ65881; BLLJ_0211
    KEGGiblm:BLLJ_0211

    Phylogenomic databases

    HOGENOMiHOG000115384
    KOiK18205
    OMAiLYSGMAD

    Enzyme and pathway databases

    BioCyciBLON565042:G1GTK-233-MONOMER
    BRENDAi3.2.1.185 851

    Family and domain databases

    InterProiView protein in InterPro
    IPR008928 6-hairpin_glycosidase_sf
    IPR012878 Glyco_hydro_127
    PfamiView protein in Pfam
    PF07944 Glyco_hydro_127, 1 hit
    SUPFAMiSSF48208 SSF48208, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHYBA1_BIFL2
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: E8MGH8
    Secondary accession number(s): G1UHC6
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
    Last sequence update: April 5, 2011
    Last modified: December 11, 2019
    This is version 43 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
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