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Protein

Endo-1,4-beta-xylanase B

Gene

xynB

Organism
Penicillium oxalicum
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Hydrolyzes birchwood xylan, beechwood xylan, and oat spelt xylan to produce short-chain xylooligosaccharides, xylopentaose, xylotriose, and xylobiose as the main products.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.2 Publications

Kineticsi

  1. KM=5 mg/ml for birchwood xylan2 Publications
  2. KM=4.6 mg/ml for beechwood xylan2 Publications
  3. KM=11.3 mg/ml for oat spelt xylan2 Publications
  1. Vmax=2 µmol/min/mg enzyme toward birchwood xylan2 Publications
  2. Vmax=2 µmol/min/mg enzyme toward beechwood xylan2 Publications
  3. Vmax=2.5 µmol/min/mg enzyme toward oat spelt xylan2 Publications

pH dependencei

Optimum pH is 5.0.2 Publications

Temperature dependencei

Optimum temperature is 50 degrees Celsius.2 Publications

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119NucleophilePROSITE-ProRule annotation1
Active sitei210Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayi
UPA00114

Protein family/group databases

CAZyiCBM1 Carbohydrate-Binding Module Family 1
GH11 Glycoside Hydrolase Family 11
mycoCLAPiXYN11A_PENOX

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase B (EC:3.2.1.8)
Short name:
Xylanase B
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase B
Gene namesi
Name:xynB
OrganismiPenicillium oxalicum
Taxonomic identifieri69781 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicillium

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000042961520 – 310Endo-1,4-beta-xylanase BAdd BLAST291

Expressioni

Inductioni

Induced by soluble xylo-oligosaccharides, with beechwood xylan being the best inducer.1 Publication

Structurei

3D structure databases

SMRiE7EF85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 223GH11PROSITE-ProRule annotationAdd BLAST191
Domaini275 – 310CBM1PROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi214 – 272Ser-richAdd BLAST59

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.180, 1 hit
InterProiView protein in InterPro
IPR035971 CBD_sf
IPR000254 Cellulose-bd_dom_fun
IPR013320 ConA-like_dom_sf
IPR013319 GH11/12
IPR018208 GH11_AS_1
IPR033119 GH11_AS_2
IPR033123 GH11_dom
IPR001137 Glyco_hydro_11
PfamiView protein in Pfam
PF00734 CBM_1, 1 hit
PF00457 Glyco_hydro_11, 1 hit
PRINTSiPR00911 GLHYDRLASE11
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001821 CBD_fun, 1 hit
SMARTiView protein in SMART
SM00236 fCBD, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF57180 SSF57180, 1 hit
PROSITEiView protein in PROSITE
PS00562 CBM1_1, 1 hit
PS51164 CBM1_2, 1 hit
PS00776 GH11_1, 1 hit
PS00777 GH11_2, 1 hit
PS51761 GH11_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E7EF85-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MISLSSVAIA LTTVVGALAL PSDQSVNLAA RQAITSSQTG TNNGYYYSFW
60 70 80 90 100
TNGAGSVSYS NGAAGQFSVN WANQGGGDFT CGKGWNPGKA QDISFSGTFT
110 120 130 140 150
PNGNAYLSIY GWTTGPLVEY YILENFGSYN PGNGMTHVGT LTSDGSDYDI
160 170 180 190 200
YKHTQVNQPS IVGTSTFDQY WSIRKNKRSS GTVTTANHFS AWASHGMNLG
210 220 230 240 250
SHNYQILSVE GYQSSGSASM TVSAGSSSSG GSGSGSGSGS GSGSGSGSQT
260 270 280 290 300
TTAGSSTGTG TGSGSGSGSG GSGGNCAAQW GQCGGQGWNG PTCCSSGTCK
310
ASNQWYSQCL
Length:310
Mass (Da):31,282
Last modified:March 8, 2011 - v1
Checksum:i0EE37FBE5422BF73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ680966 Genomic DNA Translation: ADV31286.1
KF233758 mRNA Translation: AGW24301.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ680966 Genomic DNA Translation: ADV31286.1
KF233758 mRNA Translation: AGW24301.1

3D structure databases

SMRiE7EF85
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM1 Carbohydrate-Binding Module Family 1
GH11 Glycoside Hydrolase Family 11
mycoCLAPiXYN11A_PENOX

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00114

Family and domain databases

Gene3Di2.60.120.180, 1 hit
InterProiView protein in InterPro
IPR035971 CBD_sf
IPR000254 Cellulose-bd_dom_fun
IPR013320 ConA-like_dom_sf
IPR013319 GH11/12
IPR018208 GH11_AS_1
IPR033119 GH11_AS_2
IPR033123 GH11_dom
IPR001137 Glyco_hydro_11
PfamiView protein in Pfam
PF00734 CBM_1, 1 hit
PF00457 Glyco_hydro_11, 1 hit
PRINTSiPR00911 GLHYDRLASE11
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001821 CBD_fun, 1 hit
SMARTiView protein in SMART
SM00236 fCBD, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF57180 SSF57180, 1 hit
PROSITEiView protein in PROSITE
PS00562 CBM1_1, 1 hit
PS51164 CBM1_2, 1 hit
PS00776 GH11_1, 1 hit
PS00777 GH11_2, 1 hit
PS51761 GH11_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiXYNB_PENOX
AccessioniPrimary (citable) accession number: E7EF85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: March 8, 2011
Last modified: November 22, 2017
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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