UniProtKB - E7D082 (SCX5B_MESEU)
Protein
Sodium channel neurotoxin MeuNaTxalpha-5*
Gene
N/A
Organism
Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus)
Status
Functioni
Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin inhibits inactivation of Nav1.6/SCN8A (EC(50)=790 nM) and drosophila DmNav1 (EC(50)=280 nM) (PubMed:21969612, Ref. 2). The toxin (1 µM) does not significantly shift the midpoint of activation at the two channels, but induces a significant depolarizing shift in the V(1/2) of inactivation of the channels (PubMed:21969612). Has antimicrobial activity (Ref. 2).2 Publications
Miscellaneous
Shows no effect on Nav1.2/SCN2A and Nav1.8/SCN10A (PubMed:21969612). Shows a weak inhibition of inactivation on Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A, and Nav1.7/SCN9A.1 Publication
Caution
Several genes are coding for this toxin for which the structure by NMR has been determined. The cross-references to PDB and additional information can be found in entry AC P86405.Curated
GO - Molecular functioni
- sodium channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response Source: InterPro
- pathogenesis Source: InterPro
Keywordsi
Molecular function | Antimicrobial, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium channel neurotoxin MeuNaTxalpha-5*1 PublicationAlternative name(s): MeuNaTxalpha-5bImported |
Organismi | Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus) |
Taxonomic identifieri | 34648 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | 2 PublicationsAdd BLAST | 19 | |
ChainiPRO_5007654375 | 20 – 85 | Sodium channel neurotoxin MeuNaTxalpha-5*1 PublicationAdd BLAST | 66 | |
PropeptideiPRO_0000447446 | 86 – 87 | Removed by a carboxypeptidaseCurated | 2 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 84 | 1 Publication | ||
Disulfide bondi | 35 ↔ 57 | 1 Publication | ||
Disulfide bondi | 43 ↔ 67 | 1 Publication | ||
Disulfide bondi | 47 ↔ 69 | 1 Publication |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.2 Publications
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 85 | LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST | 65 |
Domaini
Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).Curated
Sequence similaritiesi
Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.Curated
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00107, Knot1, 1 hit |
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR044062, LCN-type_CS_alpha_beta_dom IPR003614, Scorpion_toxin-like IPR036574, Scorpion_toxin-like_sf IPR018218, Scorpion_toxinL IPR002061, Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537, Toxin_3, 1 hit |
PRINTSi | PR00285, SCORPNTOXIN |
SMARTi | View protein in SMART SM00505, Knot1, 1 hit |
SUPFAMi | SSF57095, SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863, LCN_CSAB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
E7D082-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNYLILISFA LLVITGVESA RDAYIAKPHN CVYECFDAFS SYCNGVCTKN
60 70 80
GAKSGYCQIL GTYGNGCWCI ALPDNVPIRI PGKCHRR
Mass spectrometryi
Molecular mass is 7170.47 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM992828 Genomic DNA Translation: ADU05410.1 HQ170506 mRNA Translation: AEM44794.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM992828 Genomic DNA Translation: ADU05410.1 HQ170506 mRNA Translation: AEM44794.1 |
3D structure databases
SMRi | E7D082 |
ModBasei | Search... |
Family and domain databases
CDDi | cd00107, Knot1, 1 hit |
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR044062, LCN-type_CS_alpha_beta_dom IPR003614, Scorpion_toxin-like IPR036574, Scorpion_toxin-like_sf IPR018218, Scorpion_toxinL IPR002061, Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537, Toxin_3, 1 hit |
PRINTSi | PR00285, SCORPNTOXIN |
SMARTi | View protein in SMART SM00505, Knot1, 1 hit |
SUPFAMi | SSF57095, SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863, LCN_CSAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCX5B_MESEU | |
Accessioni | E7D082Primary (citable) accession number: E7D082 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 3, 2019 |
Last sequence update: | March 8, 2011 | |
Last modified: | December 2, 2020 | |
This is version 42 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families