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Protein

4-O-beta-D-mannosyl-D-glucose phosphorylase

Gene

Rumal_0852

Organism
Ruminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.UniRule annotation1 Publication

Miscellaneous

Both RaMP1 and RaMP2 (Rumal_0099) catalyze the phosphorolysis of a beta-1,4-mannosidic linkage at the non-reducing end of a substrate, but acceptor specificities are clearly different.1 Publication

Catalytic activityi

4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate.UniRule annotation1 Publication

Kineticsi

  1. KM=0.304 mM for Man-Glc1 Publication
  2. KM=5.34 mM for phosphate1 Publication
  3. KM=38.2 mM for glucose1 Publication
  4. KM=5.80 mM for Man1P1 Publication

    pH dependencei

    Optimum pH is 6.5.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18533
    BRENDAi2.4.1.281 5477

    Protein family/group databases

    CAZyiGH130 Glycoside Hydrolase Family 130

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-O-beta-D-mannosyl-D-glucose phosphorylaseUniRule annotation (EC:2.4.1.281UniRule annotation)
    Short name:
    MGPUniRule annotation
    Short name:
    Mannosylglucose phosphorylaseUniRule annotation
    Alternative name(s):
    RaMP1
    Gene namesi
    Ordered Locus Names:Rumal_0852
    OrganismiRuminococcus albus (strain ATCC 27210 / DSM 20455 / JCM 14654 / NCDO 2250 / 7)
    Taxonomic identifieri697329 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus
    Proteomesi
    • UP000006919 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004213661 – 3864-O-beta-D-mannosyl-D-glucose phosphorylaseAdd BLAST386

    Interactioni

    Subunit structurei

    Homodimer in solution.1 Publication

    Protein-protein interaction databases

    MINTiE6UIS7
    STRINGi697329.Rumal_0852

    Structurei

    Secondary structure

    1386
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi5 – 18Combined sources14
    Beta strandi28 – 30Combined sources3
    Beta strandi34 – 36Combined sources3
    Helixi41 – 43Combined sources3
    Helixi46 – 48Combined sources3
    Turni54 – 56Combined sources3
    Beta strandi62 – 64Combined sources3
    Beta strandi66 – 77Combined sources12
    Beta strandi80 – 89Combined sources10
    Beta strandi94 – 106Combined sources13
    Beta strandi120 – 122Combined sources3
    Beta strandi126 – 134Combined sources9
    Beta strandi140 – 149Combined sources10
    Beta strandi152 – 154Combined sources3
    Beta strandi158 – 175Combined sources18
    Beta strandi185 – 187Combined sources3
    Beta strandi200 – 205Combined sources6
    Beta strandi207 – 212Combined sources6
    Beta strandi219 – 226Combined sources8
    Helixi228 – 230Combined sources3
    Beta strandi232 – 234Combined sources3
    Beta strandi237 – 241Combined sources5
    Beta strandi249 – 253Combined sources5
    Beta strandi259 – 261Combined sources3
    Beta strandi264 – 275Combined sources12
    Beta strandi278 – 288Combined sources11
    Beta strandi295 – 298Combined sources4
    Helixi309 – 312Combined sources4
    Beta strandi318 – 327Combined sources10
    Beta strandi331 – 339Combined sources9
    Turni340 – 342Combined sources3
    Beta strandi343 – 350Combined sources8
    Helixi351 – 360Combined sources10
    Helixi368 – 385Combined sources18

    3D structure databases

    SMRiE6UIS7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 130 family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105HY2 Bacteria
    COG2152 LUCA
    HOGENOMiHOG000252436
    KOiK16212
    OMAiYAFYTRP
    OrthoDBiPOG091H25RO

    Family and domain databases

    Gene3Di2.115.10.20, 1 hit
    HAMAPiMF_00928 Man_Glc_phosphorylase, 1 hit
    InterProiView protein in InterPro
    IPR023296 Glyco_hydro_beta-prop_sf
    IPR028583 Man_Glc_phosphorylase
    IPR007184 Mannoside_phosphorylase
    PANTHERiPTHR34106 PTHR34106, 1 hit
    PfamiView protein in Pfam
    PF04041 Glyco_hydro_130, 1 hit
    PIRSFiPIRSF016202 PH1107, 1 hit
    SUPFAMiSSF75005 SSF75005, 1 hit

    Sequencei

    Sequence statusi: Complete.

    E6UIS7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIHEKYTEMR NEQEALLSRK NTKTSFYNGI YDRYEHPVLT REHIPLHWRY
    60 70 80 90 100
    DLNKETNPFF QERLGINAVF NAGAIKLNDR YCLVARVEGN DRKSFFAVAE
    110 120 130 140 150
    SDKGTEGFRF RQYPVCLPAL TDDETNVYDM RLTQHEDGWI YGVFCVEKSA
    160 170 180 190 200
    GTADLSEAVA SAGIARTKDL TNWERLPDLV TLRSPQQRNV TLLPEFVDGK
    210 220 230 240 250
    YAFYTRPMDG FIETGSGGGI GFGLADDITH AVIDEERMTS IRRYHTITES
    260 270 280 290 300
    KNGAGATPIK TERGWLNIAH GVRNTAAGLR YVIYCFVTDL SEPWKVIAEP
    310 320 330 340 350
    GGYLIAPFKD ERVGDVSNVV FTNGAIVDDN GDVYIYYASS DTRLHVAVSS
    360 370 380
    IDKLLDYAFN TPADALRTAE CVKQRCDLIK RNIELL
    Length:386
    Mass (Da):43,646
    Last modified:March 8, 2011 - v1
    Checksum:i6A5E711D3B184F8A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP002403 Genomic DNA Translation: ADU21379.1
    RefSeqiWP_013497557.1, NZ_JHYT01000014.1

    Genome annotation databases

    EnsemblBacteriaiADU21379; ADU21379; Rumal_0852
    KEGGiral:Rumal_0852

    Similar proteinsi

    Entry informationi

    Entry nameiMGP_RUMA7
    AccessioniPrimary (citable) accession number: E6UIS7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
    Last sequence update: March 8, 2011
    Last modified: July 18, 2018
    This is version 37 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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