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Entry version 40 (02 Dec 2020)
Sequence version 1 (08 Mar 2011)
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Protein

Isethionate sulfite-lyase

Gene

islA

Organism
Bilophila wadsworthia (strain 3_1_6)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in an anaerobic respiration pathway that converts the sulfonate taurine (2-aminoethanesulfonate) to ammonia, acetate and sulfide. Catalyzes the radical-mediated C-S bond cleavage of isethionate (2-hydroxyethanesulfonate) to form sulfite and acetaldehyde. Is not able to use any alternate organosulfonate or (S)-1,2-propanediol or choline as a substrate, showing that this enzyme is highly specific for isethionate.1 Publication

Miscellaneous

Taurine is an abundant dietary and host-derived molecule whose metabolism to hydrogen sulfide (H2S) by members of the human gut microbiota has many prominent connections to host health and disease. The human gut bacterium and opportunistic pathogen Bilophila wadsworthia produces H2S when respiring sulfite (HSO3-) released from organosulfonate substrates such as taurine and isethionate.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 14 sec(-1).1 Publication
  1. KM=8.1 mM for 2-hydroxyethane-1-sulfonate1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: alkanesulfonate degradation

    This protein is involved in the pathway alkanesulfonate degradation, which is part of Organosulfur degradation.1 Publication
    View all proteins of this organism that are known to be involved in the pathway alkanesulfonate degradation and in Organosulfur degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei1892-hydroxyethane-1-sulfonateBy similarity1
    Binding sitei1932-hydroxyethane-1-sulfonateBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei468Cysteine radical intermediateBy similarity1
    Active sitei470Proton acceptorBy similarity1
    Binding sitei6782-hydroxyethane-1-sulfonateBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionLyase

    Enzyme and pathway databases

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00338

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Isethionate sulfite-lyase1 Publication (EC:4.4.1.-1 Publication)
    Alternative name(s):
    Glycyl radical enzyme IslA1 Publication
    Short name:
    GRE IslA1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:islA1 Publication
    Synonyms:iseG
    ORF Names:HMPREF0179_00639Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBilophila wadsworthia (strain 3_1_6)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri563192 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeBilophila
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000006034 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004509411 – 830Isethionate sulfite-lyaseAdd BLAST830

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei805Glycine radicalPROSITE-ProRule annotation1

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Requires the activating protein IslB to generate the key active site glycyl radical on Gly-805 that is involved in catalysis.1 Publication

    Keywords - PTMi

    Organic radical

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Highly up-regulated in the presence of taurine or isethionate.1 Publication

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    By similarity

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    563192.HMPREF0179_00639

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini31 – 700PFLPROSITE-ProRule annotationAdd BLAST670
    Domaini707 – 830Glycine radicalPROSITE-ProRule annotationAdd BLAST124

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni468 – 4702-hydroxyethane-1-sulfonate bindingBy similarity3

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the glycyl radical enzyme (GRE) family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105C6N, Bacteria
    COG1882, LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_009096_0_1_7

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR019777, Form_AcTrfase_GR_CS
    IPR001150, Gly_radical
    IPR004184, PFL_dom

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF01228, Gly_radical, 1 hit
    PF02901, PFL-like, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00850, GLY_RADICAL_1, 1 hit
    PS51149, GLY_RADICAL_2, 1 hit
    PS51554, PFL, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    E5Y378-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTQVAEIKSP HEQRLEDNIA GKEDIYRESH KRVFKLLERF DGQKPAIDVE
    60 70 80 90 100
    RALYFTQSMA ETVGQPLVLR WAKALMNVAK NITVMVQDDQ LLLGRCGGHD
    110 120 130 140 150
    GRYGILYPEL DGDFLDIAVR DLPTRPQSPA SISPEDAKIV VEQIAPFWKG
    160 170 180 190 200
    RTYHEALNKA LPAEVHKLTY DDPDGLISRF IVNETSSFRS SIQWVHDYEV
    210 220 230 240 250
    VLKRGFNGLK QEMEEKLAAL DPASPVDQVD KRPFIEATIL VCDAIVLWAK
    260 270 280 290 300
    RHADAARKAA EACADPVRKA ELIRMAENAE HVPANPARDF YEAVQSQYFT
    310 320 330 340 350
    QMFSRLEQKT GTTISNGRMD QYFYPFYKKD MEAGILTDEK TLEYLECMWV
    360 370 380 390 400
    GMAEFIDMYI SPAGGAFNEG YAHWEAVTIG GQTPDGRDAT NDLTYLFLKS
    410 420 430 440 450
    KREFPLHYPD LAARIHSRAP ERYLWDVAET IKFGSGFPKL CNDEECIPLY
    460 470 480 490 500
    VSKGATFEEA LDYAVSGCIE IRMPNRDTYT SGGAYTNFAS AVEMALYDGK
    510 520 530 540 550
    MKKYGDVQLG IQTGDARKFK SWDEFWNAYV QQHMLLLRTT FIQQYIVIQT
    560 570 580 590 600
    RAKHFAQPMG SVLHALCRKH CIDLHQPQIP EGLNFGYFEF MGLGTVIDSL
    610 620 630 640 650
    AAIKKLVFED KKLTMDQLID ALEANFEGYE DIQQLLRTAP CYGNDDEYAD
    660 670 680 690 700
    EIGRELDRMA VSFAAKYGKE MGINNDARYV PFTSHVPFGK VVSATPNGRV
    710 720 730 740 750
    AWFPLADGSS PSHGADHNGP TAILLSNHNT KNYGMRARAA RLINVKFTPK
    760 770 780 790 800
    CVEGDAGTEK LVQFIRTWCD LKLWHIQFNV INADTLKKAQ KDPQKYRNLI
    810 820 830
    VRIAGYSAYF VDLTPDLQND LIARTGHDQM
    Length:830
    Mass (Da):93,965
    Last modified:March 8, 2011 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA0F49549EB008727
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    ADCP02000001 Genomic DNA Translation: EFV45544.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_005024906.1, NZ_KE150238.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    EFV45544; EFV45544; HMPREF0179_00639

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    ADCP02000001 Genomic DNA Translation: EFV45544.1
    RefSeqiWP_005024906.1, NZ_KE150238.1

    3D structure databases

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    SWISS-MODEL Interactive Workspace

    More...
    SWISS-MODEL-Workspacei
    Submit a new modelling project...

    Protein-protein interaction databases

    STRINGi563192.HMPREF0179_00639

    Genome annotation databases

    EnsemblBacteriaiEFV45544; EFV45544; HMPREF0179_00639

    Phylogenomic databases

    eggNOGiENOG4105C6N, Bacteria
    COG1882, LUCA
    HOGENOMiCLU_009096_0_1_7

    Enzyme and pathway databases

    UniPathwayiUPA00338

    Family and domain databases

    InterProiView protein in InterPro
    IPR019777, Form_AcTrfase_GR_CS
    IPR001150, Gly_radical
    IPR004184, PFL_dom
    PfamiView protein in Pfam
    PF01228, Gly_radical, 1 hit
    PF02901, PFL-like, 1 hit
    PROSITEiView protein in PROSITE
    PS00850, GLY_RADICAL_1, 1 hit
    PS51149, GLY_RADICAL_2, 1 hit
    PS51554, PFL, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiISLA_BILW3
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: E5Y378
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 7, 2020
    Last sequence update: March 8, 2011
    Last modified: December 2, 2020
    This is version 40 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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