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UniProtKB - E5KGE0 (BRIZ1_ARATH)
Protein
BRAP2 RING ZnF UBP domain-containing protein 1
Gene
BRIZ1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
RING-type ubiquitin E3 ligase required for seed germination and post-germination growth.
1 PublicationCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 225 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 228 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 237 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 240 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 245 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 252 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 256 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 262 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 174 – 214 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 208 – 301 | UBP-type; degeneratePROSITE-ProRule annotationAdd BLAST | 94 |
GO - Molecular functioni
- hydrolase activity Source: UniProtKB-KW
- protein heterodimerization activity Source: TAIR
- ubiquitin-protein transferase activity Source: TAIR
- zinc ion binding Source: InterPro
GO - Biological processi
- regulation of seed germination Source: TAIR
Keywordsi
Molecular function | Hydrolase, Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: BRAP2 RING ZnF UBP domain-containing protein 11 Publication (EC:2.3.2.271 Publication) |
Gene namesi | Name:BRIZ11 Publication Synonyms:RING-HCa1 Publication Ordered Locus Names:At2g42160Imported ORF Names:T24P15.7Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G42160 |
TAIRi | locus:2060045, AT2G42160 |
Pathology & Biotechi
Disruption phenotypei
Viable heterozygous plants seeds are slow to emerge from the seed coat; emerged embryos remains white with unexpanded cotyledons thus leading to growth-arrested seedlings.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000447000 | 1 – 488 | BRAP2 RING ZnF UBP domain-containing protein 1Add BLAST | 488 |
Proteomic databases
PaxDbi | E5KGE0 |
ProteomicsDBi | 191336 [E5KGE0-1] |
PTM databases
iPTMneti | E5KGE0 |
Interactioni
Subunit structurei
GO - Molecular functioni
- protein heterodimerization activity Source: TAIR
Protein-protein interaction databases
STRINGi | 3702.AT2G42160.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 453 – 488 | DisorderedSequence analysisAdd BLAST | 36 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 370 – 418 | Sequence analysisAdd BLAST | 49 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 174 – 214 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 208 – 301 | UBP-type; degeneratePROSITE-ProRule annotationAdd BLAST | 94 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0804, Eukaryota |
HOGENOMi | CLU_009969_1_0_1 |
OMAi | SGNLWIC |
OrthoDBi | 659103at2759 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011422, BRAP2 IPR033273, BRIZ1/BRIZ2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
PANTHERi | PTHR24007:SF10, PTHR24007:SF10, 1 hit |
Pfami | View protein in Pfam PF07576, BRAP2, 1 hit PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00290, ZnF_UBP, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit PS50271, ZF_UBP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: E5KGE0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFILRVHSVD SERPISVEEE ESGFTYASKR AQPPLKLIQP SLKLTDRKGL
60 70 80 90 100
IHLYRKSSHS SLPNPSSRST TLFIVAVPNY LSSLDFIRFC DSRISQVSDI
110 120 130 140 150
LFIRNDGMED RYSVLITFSD QSEADGFYNN LNGKKFAPSE AEVCHILYVM
160 170 180 190 200
SVEHTEFDEV AAEAPTGFTE LPTCPICLER LDPDTSGIVS TLCDHSFQCS
210 220 230 240 250
CTSKWTYLSC QVCRLCQQQD EILNCSICGK TENVWACLVC GFVGCGRYKE
260 270 280 290 300
GHSIRHWKET HHCYSLDLRT QQIWDYVGDS YVHRLNHSKI DGKSVEMSTS
310 320 330 340 350
CLSHQGDCGL CECSEDTGIS GAIFNSKVDS IVIEYNDLLA SQLKGQRQYY
360 370 380 390 400
ESLIVEARSK QESSIAEAVE QIVVNTMQEL QNKIEKCEEE KSGITEVNTK
410 420 430 440 450
LIKEQDTWRK KAKEIEEREA ALLGSKDEMI TDLQEQIRDI TVFIEAKKTL
460 470 480
KKMSSDTDGI REGTVLPVPI SPEPVSSVRR QKKSNRRK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AYK4 | A0A1P8AYK4_ARATH | Zinc finger (Ubiquitin-hydrolase) d... | BRIZ1 At2g42160, T24P15.7, T24P15_7 | 449 | Annotation score: | ||
A0A1P8AYR4 | A0A1P8AYR4_ARATH | Zinc finger (Ubiquitin-hydrolase) d... | BRIZ1 At2g42160, T24P15.7, T24P15_7 | 403 | Annotation score: |
Sequence cautioni
The sequence AAB88642 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 396 | E → G in BAC43163 (PubMed:11910074).Curated | 1 | |
Sequence conflicti | 396 | E → G in AAO42962 (PubMed:14593172).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060129 | 1 – 376 | Missing in isoform 2. Add BLAST | 376 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HQ127733 mRNA Translation: ADQ57814.1 AC002561 Genomic DNA Translation: AAB88642.1 Sequence problems. CP002685 Genomic DNA Translation: AEC10081.1 AK118562 mRNA Translation: BAC43163.1 BT004716 mRNA Translation: AAO42962.1 |
PIRi | T00926 |
RefSeqi | NP_181746.2, NM_129779.4 [E5KGE0-1] |
Genome annotation databases
EnsemblPlantsi | AT2G42160.1; AT2G42160.1; AT2G42160 [E5KGE0-1] |
GeneIDi | 818816 |
Gramenei | AT2G42160.1; AT2G42160.1; AT2G42160 [E5KGE0-1] |
KEGGi | ath:AT2G42160 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HQ127733 mRNA Translation: ADQ57814.1 AC002561 Genomic DNA Translation: AAB88642.1 Sequence problems. CP002685 Genomic DNA Translation: AEC10081.1 AK118562 mRNA Translation: BAC43163.1 BT004716 mRNA Translation: AAO42962.1 |
PIRi | T00926 |
RefSeqi | NP_181746.2, NM_129779.4 [E5KGE0-1] |
3D structure databases
SMRi | E5KGE0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G42160.1 |
PTM databases
iPTMneti | E5KGE0 |
Proteomic databases
PaxDbi | E5KGE0 |
ProteomicsDBi | 191336 [E5KGE0-1] |
Genome annotation databases
EnsemblPlantsi | AT2G42160.1; AT2G42160.1; AT2G42160 [E5KGE0-1] |
GeneIDi | 818816 |
Gramenei | AT2G42160.1; AT2G42160.1; AT2G42160 [E5KGE0-1] |
KEGGi | ath:AT2G42160 |
Organism-specific databases
Araporti | AT2G42160 |
TAIRi | locus:2060045, AT2G42160 |
Phylogenomic databases
eggNOGi | KOG0804, Eukaryota |
HOGENOMi | CLU_009969_1_0_1 |
OMAi | SGNLWIC |
OrthoDBi | 659103at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:E5KGE0 |
Gene expression databases
ExpressionAtlasi | E5KGE0, baseline and differential |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011422, BRAP2 IPR033273, BRIZ1/BRIZ2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR001607, Znf_UBP |
PANTHERi | PTHR24007:SF10, PTHR24007:SF10, 1 hit |
Pfami | View protein in Pfam PF07576, BRAP2, 1 hit PF02148, zf-UBP, 1 hit |
SMARTi | View protein in SMART SM00290, ZnF_UBP, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit PS50271, ZF_UBP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BRIZ1_ARATH | |
Accessioni | E5KGE0Primary (citable) accession number: E5KGE0 Secondary accession number(s): O48524, Q8GWY2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 8, 2019 |
Last sequence update: | February 8, 2011 | |
Last modified: | February 23, 2022 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways