UniProtKB - E5FYH1 (TOPZ1_MOUSE)
Protein
Protein TOPAZ1
Gene
Topaz1
Organism
Mus musculus (Mouse)
Status
Functioni
Important for normal spermatogenesis and male fertility. Specifically required for progression to the post-meiotic stages of spermatocyte development. Seems to be necessary for normal expression levels of a number of testis-expressed gene transcripts, although its role in this process is unclear.1 Publication
GO - Biological processi
- apoptotic process Source: MGI
- ncRNA transcription Source: MGI
- positive regulation of meiotic cell cycle phase transition Source: MGI
- spermatid development Source: MGI
- spermatocyte division Source: MGI
Keywordsi
Biological process | Differentiation, Spermatogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Protein TOPAZ1CuratedAlternative name(s): Testis- and ovary-specific PAZ domain-containing protein 11 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3779933, Topaz1 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Cytosol
- cytosol Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Males are infertile, while female fertility is not affected. Testis size is significantly reduced with the phenotype apparent from postnatal day 28 (P28) onwards. Testicular histology appears to be normal before P28, but then cell aggregates begin to accumulate in the center of seminiferous tubules. Sperm development arrests at the post-meiotic round spermatid stage, associated with increased apoptosis. Adult testes completely lack round spermatids and spermatozoa. Meiosis appears to be normal up to the pachytene stage, with no apparent effect on synaptonemal complex formation or meiotic sex chromosome inactivation. However, chromosome alignment on the metaphase plate may be abnormal. In stage P20 testes, 99 gene transcripts show abnormal expression levels, including around 10 lincRNAs.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000416061 | 1 – 1653 | Protein TOPAZ1Add BLAST | 1653 |
Proteomic databases
PaxDbi | E5FYH1 |
PRIDEi | E5FYH1 |
ProteomicsDBi | 258815 |
PTM databases
iPTMneti | E5FYH1 |
PhosphoSitePlusi | E5FYH1 |
Expressioni
Tissue specificityi
Restricted to testis, where it localizes to germ cells.2 Publications
Developmental stagei
In both developing female and male gonads, first detected at 12.5 dpc, expression increases until 16.5 dpc and then drops (PubMed:22069478). Divergence between male and female expression starts around 5 days after birth (PubMed:22069478). In ovaries, expression decreases and becomes hardly detectable in adult ovaries, while in testis, expression increases 5 days after birth and becomes strong in the adult (PubMed:22069478). In both males and females, expression is restricted to germ cells (PubMed:22069478). In developing male germ cells, highly expressed at the spermatocyte stage; has little or no expression in round spermatids, elongated spermatids or spermatozoa (PubMed:26358182).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000094985, Expressed in spermatocyte and 16 other tissues |
Interactioni
Protein-protein interaction databases
BioGRIDi | 582959, 7 interactors |
STRINGi | 10090.ENSMUSP00000136304 |
Miscellaneous databases
RNActi | E5FYH1, protein |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QPIV, Eukaryota |
GeneTreei | ENSGT00390000012495 |
HOGENOMi | CLU_003190_0_0_1 |
InParanoidi | E5FYH1 |
OMAi | NIFMEYY |
OrthoDBi | 347766at2759 |
TreeFami | TF338635 |
Family and domain databases
InterProi | View protein in InterPro IPR038952, TOPAZ1 IPR029435, TOPAZ1_dom |
PANTHERi | PTHR35671, PTHR35671, 1 hit |
Pfami | View protein in Pfam PF14669, Asp_Glu_race_2, 1 hit |
i Sequence
Sequence statusi: Complete.
E5FYH1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRPPATPPDP TKEPGCKGTT RGLRKRGLPL TPEPGEGGGC SLEARGCEEE
60 70 80 90 100
SRQKQRMVTQ ASGREETEGD KLAKENGKIT EAPSDDPQPG TDLVRKTSIT
110 120 130 140 150
SSESLQTVEC SEFQNMAFLQ SLDKEELGEG IKRRMRIKKC KSLENPPLEI
160 170 180 190 200
TKNEATQNIK VEFQDELFKN TPKHSCNSLS PGVEKNCSFE LHDYSFLHSE
210 220 230 240 250
GCNNENNFED KPHDVCLHTE ENSLKLKKEN LRNLAEKDDT RTTTKLLKTE
260 270 280 290 300
KSVIASKLLL EESHLYQNKN NGLTSCLQSE KNKYSVEENN TGRKHRKKMK
310 320 330 340 350
SGKEEKNINL TLSNVCNNSV LVLQENQMGM EGKEAETLEP KKSFLKALRK
360 370 380 390 400
INHNTLPPVD HLCLPKTVGK TSSRHHINAM LQKTLESSLK EDIKNTSESL
410 420 430 440 450
GCKRIEPEKY NKSMISSTVK SPSDGHHMEK RSPRGDLRSE TEESKVSCCR
460 470 480 490 500
TIPMTGKRVW PFYSCARISA QCWKKTSLSD LNYSLLGPLE NVRQHDSIIH
510 520 530 540 550
QMNQTHLPDS KLLQPSLTER TTESSRKEMY DSDLSCLSSV SSVESTVMDI
560 570 580 590 600
KEAMSHDKKT KLEEPSRNGA EVVSNATEDT QLSNITQSLT GNKKRKGNLS
610 620 630 640 650
KLNLTVASQE SQETNNCANK TVHRKVCITK QTLVAPDLVK ILNTGRLTNF
660 670 680 690 700
KIPLLKNKTG KRGAVSARSS EREAYSPLEL LDSLSGVEAK QNRNKENICT
710 720 730 740 750
TSGPQSLNLH SCIAPGQASS HSFYNKNSCT SSSFTKKGYD NKACNHISEP
760 770 780 790 800
GNIISNKESI SMKIENNTFS CDLGYIDQSS FCSKKQEAFV PISSEISGRK
810 820 830 840 850
MTKSISELKL GFPDILKAYE DDVLLIDVIQ DDPELFGISS EGDLSFASEV
860 870 880 890 900
SKISQEPRVS EDQPTADFKH MHLPGKKEPG DLSKEVALLD PGLLKLESCP
910 920 930 940 950
SLSAAKEPQH DPEGAAISLE ATEETVVSGS LEGLSEQARA SDSDAKCISS
960 970 980 990 1000
DKATVMEEQE STHEIFKSKD SRNVESATEC QLATLGPNPL CSSALPVNLS
1010 1020 1030 1040 1050
SHQDVVSTPW INDVRFPGRH SVLQLQNPET CEIFKREKNM GVFQKPLGLL
1060 1070 1080 1090 1100
IPHRYCKLHF NTLRGCERAQ CKFVHVPEQG DEKICMDVFR KYISVNEQRL
1110 1120 1130 1140 1150
LHRAAYIFLE YYRKFPPGIH FSLQVLNDLL ISLLKHCLLK EVFQVVQLSI
1160 1170 1180 1190 1200
MAKMLPALKI LLKIFEYVAA MKLRNAVPAL IEIFCKFIEA GMVPDPEHLN
1210 1220 1230 1240 1250
YIVKLLHQAQ ASQQEISAVL QAKSRLRVRQ LKKNWKCDLD SALSEVETCK
1260 1270 1280 1290 1300
EKSDWTKLGN LYISIKMSCE EFADLQRFCA CVAETLTEDY KEERPGVPFC
1310 1320 1330 1340 1350
EFAETVSKDP QYSEVDKTLL GRIGISAVYF YHRLLLWAKG RKVLDILYEL
1360 1370 1380 1390 1400
KIHFTSLKGL TGPEKEAPRC QIVNVAAEIF IKSGSLDGAI WVLRESEWII
1410 1420 1430 1440 1450
NTPLWPCDRM DVLNRHNLLC TIAHEILGKN LYKQTFEVLR NLPSFQNSQE
1460 1470 1480 1490 1500
MMGVSQSSLL FNELLDACIE SNSLGISSSV AEFMVAKSIP IDFSFLRRLI
1510 1520 1530 1540 1550
TSLGRSCLWL KARAHYKSAL SLGCYPPLEG NLHRKLLLVP SYLSEIEMLL
1560 1570 1580 1590 1600
AMEIFLVSNA SGIQSAGMGA PTQVLQIVLK RCEESKSRSK DEYQAAVERL
1610 1620 1630 1640 1650
VMAARISDPK LFIKHMTVNI NKEQVYSLEH CSALKWLKEN MKWAGKVWLF
TNH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM631980 mRNA Translation: ADK36691.1 AC124778 Genomic DNA No translation available. AC125374 Genomic DNA No translation available. |
CCDSi | CCDS57718.1 |
RefSeqi | NP_001186665.1, NM_001199736.1 |
Genome annotation databases
Ensembli | ENSMUST00000178679; ENSMUSP00000136304; ENSMUSG00000094985 |
GeneIDi | 671232 |
KEGGi | mmu:671232 |
UCSCi | uc012hcy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM631980 mRNA Translation: ADK36691.1 AC124778 Genomic DNA No translation available. AC125374 Genomic DNA No translation available. |
CCDSi | CCDS57718.1 |
RefSeqi | NP_001186665.1, NM_001199736.1 |
3D structure databases
SMRi | E5FYH1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 582959, 7 interactors |
STRINGi | 10090.ENSMUSP00000136304 |
PTM databases
iPTMneti | E5FYH1 |
PhosphoSitePlusi | E5FYH1 |
Proteomic databases
PaxDbi | E5FYH1 |
PRIDEi | E5FYH1 |
ProteomicsDBi | 258815 |
Protocols and materials databases
Antibodypediai | 64655, 24 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000178679; ENSMUSP00000136304; ENSMUSG00000094985 |
GeneIDi | 671232 |
KEGGi | mmu:671232 |
UCSCi | uc012hcy.1, mouse |
Organism-specific databases
CTDi | 375337 |
MGIi | MGI:3779933, Topaz1 |
Phylogenomic databases
eggNOGi | ENOG502QPIV, Eukaryota |
GeneTreei | ENSGT00390000012495 |
HOGENOMi | CLU_003190_0_0_1 |
InParanoidi | E5FYH1 |
OMAi | NIFMEYY |
OrthoDBi | 347766at2759 |
TreeFami | TF338635 |
Miscellaneous databases
BioGRID-ORCSi | 671232, 2 hits in 53 CRISPR screens |
ChiTaRSi | Topaz1, mouse |
PROi | PR:E5FYH1 |
RNActi | E5FYH1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000094985, Expressed in spermatocyte and 16 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR038952, TOPAZ1 IPR029435, TOPAZ1_dom |
PANTHERi | PTHR35671, PTHR35671, 1 hit |
Pfami | View protein in Pfam PF14669, Asp_Glu_race_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOPZ1_MOUSE | |
Accessioni | E5FYH1Primary (citable) accession number: E5FYH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2012 |
Last sequence update: | February 8, 2011 | |
Last modified: | April 7, 2021 | |
This is version 53 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot