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Protein

Glutamate 5-kinase

Gene

proB

Organism
Caldicellulosiruptor owensensis (strain ATCC 700167 / DSM 13100 / OL)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATPUniRule annotation1
Binding sitei53SubstrateUniRule annotation1
Binding sitei140SubstrateUniRule annotation1
Binding sitei152Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 173ATPUniRule annotation2
Nucleotide bindingi214 – 220ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCOWE632518:G1GOW-1412-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotationImported
Ordered Locus Names:Calow_1390Imported
OrganismiCaldicellulosiruptor owensensis (strain ATCC 700167 / DSM 13100 / OL)Imported
Taxonomic identifieri632518 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
Proteomesi
  • UP000006889 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi632518.Calow_1390

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 238AA_kinaseInterPro annotationAdd BLAST231

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

E4Q2J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDFKNVKKV VVKVGTSTVT YPTGKLNLSR LEKLARVLSD IKNEGRDVVL
60 70 80 90 100
VTSGAVASGL GRLGLTKNHK TTQEKQALAA IGQGILMQIY EKLFGEYGVV
110 120 130 140 150
VAQVLLTKDV VDEEKKMLNV KNTFEYLFKY DAIPIVNEND VVAIEELEFG
160 170 180 190 200
DNDTLSAYVA TIIDADLLII LSDIDGLYSC DPRIDKSAKL IKEVFEIDSY
210 220 230 240 250
IESIAGGAGT VNSTGGMQTK IEAAKIAMQH GIPMVIANGE NPSVVKEILE
260
GKEIGTLFVN KNTVSR
Length:266
Mass (Da):28,941
Last modified:February 8, 2011 - v1
Checksum:i374E5A14FE79051C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002216 Genomic DNA Translation: ADQ04941.1
RefSeqiWP_013412303.1, NC_014657.1

Genome annotation databases

EnsemblBacteriaiADQ04941; ADQ04941; Calow_1390
KEGGicow:Calow_1390

Similar proteinsi

Entry informationi

Entry nameiE4Q2J9_CALOW
AccessioniPrimary (citable) accession number: E4Q2J9
Entry historyiIntegrated into UniProtKB/TrEMBL: February 8, 2011
Last sequence update: February 8, 2011
Last modified: February 28, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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