UniProtKB - E4PMA5 (GGOP_MARAH)
Protein
Glucosylglycerol phosphorylase
Gene
gtfA
Organism
Marinobacter adhaerens (strain DSM 23420 / HP15)
Status
Functioni
Catalyzes the reversible phosphorolysis of 2-O-alpha-D-glucosylglycerol with retention of the anomeric configuration, forming alpha-D-glucose 1-phosphate and glycerol. Has most likely a catabolic role, either regulating the intracellular levels of glucosylglycerol, which acts as a compatible solute, or degrading it when the environmental conditions change. Cannot catalyze the phosphorolysis of sucrose or glucosylglycerate.1 Publication
Catalytic activityi
- EC:2.4.1.3591 Publication
Kineticsi
kcat is 14 sec(-1) towards 2-O-(alpha-D-glucopyranosyl)glycerol for the phosphorolytic direction (at pH 6.5 and 37 degrees Celsius). kcat is 14 sec(-1) towards phosphate for the phosphorolytic direction (at pH 6.5 and 37 degrees Celsius). kcat is 36 sec(-1) towards alpha-D-glucose 1-phosphate for the synthetic direction (at pH 6.5 and 37 degrees Celsius). kcat is 39 sec(-1) towards glycerol for the synthetic direction (at pH 6.5 and 37 degrees Celsius).1 Publication
- KM=3.9 mM for 2-O-(alpha-D-glucopyranosyl)glycerol (at pH 6.5 and 37 degrees Celsius)1 Publication
- KM=10 mM for phosphate (at pH 6.5 and 37 degrees Celsius)1 Publication
- KM=2.5 mM for alpha-D-glucose 1-phosphate (at pH 6.5 and 37 degrees Celsius)1 Publication
- KM=7.0 mM for glycerol (at pH 6.5 and 37 degrees Celsius)1 Publication
pH dependencei
Optimum pH is 6.5.1 Publication
Temperature dependencei
Optimum temperature is 37 degrees Celsius. Full activity is retained after incubating at up to 45 degrees Celsius for 10 minutes, while only 46% is left after incubating at 50 degrees Celsius. The protein is completely denatured after incubating at higher temperatures.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 190 | NucleophileBy similarity | 1 | |
Binding sitei | 194 | Substrate1 Publication | 1 | |
Active sitei | 231 | Proton donorBy similarity | 1 | |
Binding sitei | 336 | Substrate1 Publication | 1 |
GO - Molecular functioni
- 1,4-alpha-oligoglucan phosphorylase activity Source: InterPro
- transferase activity, transferring glycosyl groups Source: UniProtKB
GO - Biological processi
- glucosylglycerol metabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism |
Enzyme and pathway databases
BioCyci | MADH225937:G1GOU-2842-MONOMER MetaCyc:MONOMER-20515 |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Glucosylglycerol phosphorylase1 Publication (EC:2.4.1.3591 Publication)Short name: GGoP1 Publication Alternative name(s): 2-O-alpha-D-glucopyranosylglycerol:phosphate alpha-D-glucosyltransferase1 Publication 2-O-alpha-D-glucosylglycerol phosphorylase (retaining)1 Publication |
Gene namesi | Name:gtfAImported Ordered Locus Names:HP15_2853Imported |
Organismi | Marinobacter adhaerens (strain DSM 23420 / HP15) |
Taxonomic identifieri | 225937 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Marinobacter › |
Proteomesi |
|
Pathology & Biotechi
Biotechnological usei
Might be an attractive biocatalyst for the production of the osmolyte glucosylglycerol, which is currently produced on industrial scale by exploiting a side activity of the closely related sucrose phosphorylase. GGoP from M.adhaerens may offer far higher productivities due to its high specific activity in the synthesis direction. Glucosylglycerol is commercially distributed as a moisturising agent in cosmetics and can also serve as a stabilizing agent in protein formulations, or even as a functional food.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 194 | Y → A: 2.7% of wild-type catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 333 | A → D: 0.3% of wild-type catalytic activity. Does not gain activity on sucrose. 1 Publication | 1 | |
Mutagenesisi | 336 | Q → A: 2.6% of wild-type catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000444046 | 1 – 480 | Glucosylglycerol phosphorylaseAdd BLAST | 480 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
HOGENOMi | CLU_021358_1_0_6 |
OMAi | CFMLEET |
Family and domain databases
CDDi | cd11355, AmyAc_Sucrose_phosphorylase, 1 hit |
InterProi | View protein in InterPro IPR006047, Glyco_hydro_13_cat_dom IPR017853, Glycoside_hydrolase_SF IPR016377, Sucrose_GGa_phosphorylase-rel IPR022527, Sucrose_phospho |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit |
PIRSFi | PIRSF003059, Sucrose_phosphorylase, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
TIGRFAMsi | TIGR03852, sucrose_gtfA, 1 hit |
i Sequence
Sequence statusi: Complete.
E4PMA5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLKNAVQLI CYPDRIGNNL KDLYTVVDTH LSEAIGGLHI LPFFPSNADG
60 70 80 90 100
GFSPLTHKEV DPKVGTWDDI EAFTAKYDLC VDLTVNHISD ESPEFTDFIA
110 120 130 140 150
NGFDSEYADL FVHVDKFGEI SPDDMAKIHI RKEKEPFREV TLSDGTKTRV
160 170 180 190 200
WCTFTEQQID LNYESDLAYQ LMESYIGFLT SKGVNLLRLD AFGYTTKRIG
210 220 230 240 250
TSCFLVEPEV YQILDWVNQV ALKHGAECLP EVHDHTSYQY AISRRNMHPY
260 270 280 290 300
GFALPPLLLY SLLDANSTYL KNWLRMCPRN MVTVLDTHDG ICIPDVEGVL
310 320 330 340 350
PDEKIKVLID NIDARSADPI MRRSAANIHS VGAIYQLTCT FYDALMQNDD
360 370 380 390 400
AYIAARAIQF FTPGIPQVYY VGLLAGCNDH ELMEQSGELR DINRHYYTLE
410 420 430 440 450
EVEQDIQKPV VQRLLSLMKF RSNYPAFDGH FELNYSNNSS VAMAWRHGDY
460 470 480
YCHLFVDLNF KTVKVTYTDV ETGETRHLEC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001978 Genomic DNA Translation: ADP98617.1 |
RefSeqi | WP_014578108.1, NC_017506.1 |
Genome annotation databases
EnsemblBacteriai | ADP98617; ADP98617; HP15_2853 |
KEGGi | mad:HP15_2853 |
PATRICi | fig|225937.3.peg.2880 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001978 Genomic DNA Translation: ADP98617.1 |
RefSeqi | WP_014578108.1, NC_017506.1 |
3D structure databases
SMRi | E4PMA5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 225937.HP15_2853 |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Genome annotation databases
EnsemblBacteriai | ADP98617; ADP98617; HP15_2853 |
KEGGi | mad:HP15_2853 |
PATRICi | fig|225937.3.peg.2880 |
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
HOGENOMi | CLU_021358_1_0_6 |
OMAi | CFMLEET |
Enzyme and pathway databases
BioCyci | MADH225937:G1GOU-2842-MONOMER MetaCyc:MONOMER-20515 |
Family and domain databases
CDDi | cd11355, AmyAc_Sucrose_phosphorylase, 1 hit |
InterProi | View protein in InterPro IPR006047, Glyco_hydro_13_cat_dom IPR017853, Glycoside_hydrolase_SF IPR016377, Sucrose_GGa_phosphorylase-rel IPR022527, Sucrose_phospho |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit |
PIRSFi | PIRSF003059, Sucrose_phosphorylase, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
TIGRFAMsi | TIGR03852, sucrose_gtfA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GGOP_MARAH | |
Accessioni | E4PMA5Primary (citable) accession number: E4PMA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 23, 2018 |
Last sequence update: | February 8, 2011 | |
Last modified: | December 2, 2020 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries