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Entry version 32 (02 Jun 2021)
Sequence version 1 (08 Feb 2011)
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Protein

Pumilio homolog 2

Gene

pum2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of deadenylase complexes leading to translational inhibition and mRNA degradation (PubMed:21098481).

Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.

By similarity1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRNA-binding
Biological processTranslation regulation

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Pumilio homolog 2Curated
Short name:
Pumilio-2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pum2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiXenopus laevis (African clawed frog)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri8355 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

Xenopus laevis and tropicalis biology and genomics resource

More...
Xenbasei
XB-GENE-6486498, pum2.L

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004331591 – 1173Pumilio homolog 2Add BLAST1173

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
E3WDQ9

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of a complex with papd4, sympk, tacc3, parn, dazl and cpeb1 (PubMed:21098481).

1 Publication

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
E3WDQ9, 1 interactor

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
E3WDQ9

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini815 – 1155PUM-HDPROSITE-ProRule annotationAdd BLAST341
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati835 – 870Pumilio 1PROSITE-ProRule annotationAdd BLAST36
Repeati871 – 906Pumilio 2PROSITE-ProRule annotationAdd BLAST36
Repeati907 – 942Pumilio 3PROSITE-ProRule annotationAdd BLAST36
Repeati943 – 978Pumilio 4PROSITE-ProRule annotationAdd BLAST36
Repeati979 – 1014Pumilio 5PROSITE-ProRule annotationAdd BLAST36
Repeati1015 – 1050Pumilio 6PROSITE-ProRule annotationAdd BLAST36
Repeati1051 – 1086Pumilio 7PROSITE-ProRule annotationAdd BLAST36
Repeati1087 – 1129Pumilio 8PROSITE-ProRule annotationAdd BLAST43
Repeati1130 – 1167Pumilio 9PROSITE-ProRule annotationAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni41 – 68DisorderedSequence analysisAdd BLAST28
Regioni265 – 296DisorderedSequence analysisAdd BLAST32
Regioni480 – 518DisorderedSequence analysisAdd BLAST39
Regioni592 – 662DisorderedSequence analysisAdd BLAST71
Regioni730 – 759DisorderedSequence analysisAdd BLAST30
Regioni850 – 854Adenine-nucleotide binding in RNA targetBy similarity5
Regioni886 – 890Uracil-nucleotide binding in RNA targetBy similarity5
Regioni922 – 926Adenine-nucleotide binding in RNA targetBy similarity5
Regioni958 – 962Non-specific-nucleotide binding in RNA targetBy similarity5
Regioni994 – 998Adenine-nucleotide binding in RNA targetBy similarity5
Regioni1030 – 1034Uracil-nucleotide binding in RNA targetBy similarity5
Regioni1066 – 1070Guanine-nucleotide binding in RNA targetBy similarity5
Regioni1109 – 1113Uracil-nucleotide binding in RNA targetBy similarity5

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. RNA-binding occurs on the concave side of the surface. Pum2 is composed of 8 pumilio repeats of 36 residues; each repeat binds a single nucleotide in its RNA target. Residues at positions 12 and 16 of the pumilio repeat bind each RNA base via hydrogen bonding or van der Waals contacts with the Watson-Crick edge, while the amino acid at position 13 makes a stacking interaction. The recognition of RNA by pumilio repeats is base specific: cysteine and glutamine at position 12 and 16, respectively, bind adenine; asparagine and glutamine bind uracil; and serine and glutamate bind guanine.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

Database of Orthologous Groups

More...
OrthoDBi
207351at2759

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07920, Pumilio, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.25.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR033133, PUM-HD
IPR033712, Pumilio_RNA-bd
IPR001313, Pumilio_RNA-bd_rpt

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00806, PUF, 8 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00025, Pumilio, 8 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48371, SSF48371, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50302, PUM, 9 hits
PS50303, PUM_HD, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

E3WDQ9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNVPCVVGMN EVAWQESRGI MHASSGQEAL GVGVGMVPSG VSSAGQAHGN
60 70 80 90 100
NPHAMPPGAP SAQVPLSGRS QDDATVGYFF QRQAGEQLNG YSNKHRWPTG
110 120 130 140 150
DSIDAAVRSV DEMNHDFQAL ALESRGMGEL LPAKKFWEHE DPAKDGQKGM
160 170 180 190 200
FLADEWRENT WGASHHSMSQ PIMVQRRPGQ GFHGNHDVAS VLSPRSESGG
210 220 230 240 250
LGVSMVEYVL SSSPADKLDP RFRKGVFSVR DCELDGPEKG EQKCKASPFE
260 270 280 290 300
EEKKRDLISA DTDNVSKLNG RGLPNGIESD CKDFNRTPGS RQASPTEITE
310 320 330 340 350
RIGPNSISAE VLGQHQNPMS NKPLSDEFPS TESQNLDGME QVGLHSLQFD
360 370 380 390 400
YPGNQIQMDS AGAGVGLFDY NTQQQMFQRQ NALTVQQLTA AQQQQQQFTL
410 420 430 440 450
AAAQQPHLAG MFSTGLAPAA FVPNPYIISA GHPGADPYTT LAGPAVVSPQ
460 470 480 490 500
YYGIPWGVYP AGLFQQQAAA AAQAANSNNQ QAATQASQGQ QQVMRATSNQ
510 520 530 540 550
RPLTPNQAQQ GQQPESLAAA NQAQIFGQGL ATSMPGYQLL TPTAYYDQTG
560 570 580 590 600
ALVVGPGARA GLAAQVRLVA SAPVLLSPAA AQAATATGAN SLTGATNGMF
610 620 630 640 650
RQMGPQQQQQ QQQQQHQQQQ QQPNANLHSN SFYGNSTMSN NSQSSSLFSP
660 670 680 690 700
GPGQPGSTSL GFGSSNSLGA AIGSAFGGFG SSVGNSAGSS GSRRDSLSAS
710 720 730 740 750
CDLYKRSSSS LAAIGQPYYN SLGFSSSPSP ISMPLPSQTS GHSLTPPPSL
760 770 780 790 800
SSHGSSSSLH LGGLTNGSGR YFSAAPGAEA KYRSAANTSS FFSSNSQLFP
810 820 830 840 850
PSRLRYNRAD IMPSGRSRLL EDFRNNRFPN LQLRDLMGHI VEFSQDQHGS
860 870 880 890 900
RFIQQKLERA SPAERQLVFS EILQAAYQLM TDVFGNYVIQ KFFEFGSMDQ
910 920 930 940 950
KLALATRIRG HVLPLALQMY GCRVIQKALE SISTDQQSEM VRELDGHVLK
960 970 980 990 1000
CVKDQNGNHV VQKCIECVTP QSLHFIIEAF KGQVYVLSTH PYGCRVIQRI
1010 1020 1030 1040 1050
LEHCTPEQTL PILEELHQST EQLVQDQYGN YVIQHVLEHG RSDDKSKIVC
1060 1070 1080 1090 1100
EVRGQVLVLS QHKFASNVVE KCVTHSSRTE RAFLIDEICC QNDGPHSALY
1110 1120 1130 1140 1150
TMMKDQYANY VVQKMIDMAE PAQRKIIMHK IRPHITTLRK YTYGKHILAK
1160 1170
LEKYYMKNSP DLGLLVGPSN GML
Length:1,173
Mass (Da):127,103
Last modified:February 8, 2011 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i79C67A01718ABD0F
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB565475 mRNA Translation: BAJ41096.1

NCBI Reference Sequences

More...
RefSeqi
NP_001233239.1, NM_001246310.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
100653503

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
xla:100653503

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB565475 mRNA Translation: BAJ41096.1
RefSeqiNP_001233239.1, NM_001246310.1

3D structure databases

SMRiE3WDQ9
ModBaseiSearch...

Protein-protein interaction databases

IntActiE3WDQ9, 1 interactor

Proteomic databases

MaxQBiE3WDQ9

Genome annotation databases

GeneIDi100653503
KEGGixla:100653503

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
100653503
XenbaseiXB-GENE-6486498, pum2.L

Phylogenomic databases

OrthoDBi207351at2759

Family and domain databases

CDDicd07920, Pumilio, 1 hit
Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989, ARM-like
IPR016024, ARM-type_fold
IPR033133, PUM-HD
IPR033712, Pumilio_RNA-bd
IPR001313, Pumilio_RNA-bd_rpt
PfamiView protein in Pfam
PF00806, PUF, 8 hits
SMARTiView protein in SMART
SM00025, Pumilio, 8 hits
SUPFAMiSSF48371, SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50302, PUM, 9 hits
PS50303, PUM_HD, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPUM2_XENLA
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: E3WDQ9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2015
Last sequence update: February 8, 2011
Last modified: June 2, 2021
This is version 32 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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