UniProtKB - E3P6N3 (CYT_AUSSU)
Protein
AsCystatin
Gene
N/A
Organism
Austrelaps superbus (Lowland copperhead snake) (Hoplocephalus superbus)
Status
Functioni
Recombinant AsCystatin inhibits various C1 cysteine proteases including cathepsin L (Ki is 0.89 pM), papain (Ki is 1.74 pM) and cathepsin B (Ki is 0.69 nM). This activity has also been observed in the crude venom. This protein has no toxic activity and its function in the venom is unknown. It may play a role as a housekeeping or regulatory protein.1 Publication
Miscellaneous
The recombinant protein does not inhibit calpain-1 (CAPN1), a C2 family cysteine protease and legumain (LGMN), a C13 family cysteine protease. Does not provoke cell death (PC3 prostate cancer cells) following continuous exposure to 6 µM for 24 hours (PubMed:21172403).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 29 | Reactive siteBy similarity | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Keywordsi
Molecular function | Protease inhibitor, Thiol protease inhibitor |
Protein family/group databases
MEROPSi | I25.012 |
Names & Taxonomyi
Protein namesi | Recommended name: AsCystatinShort name: AsCys Short name: Cystatin |
Organismi | Austrelaps superbus (Lowland copperhead snake) (Hoplocephalus superbus) |
Taxonomic identifieri | 29156 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Austrelaps |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 | G → S: 1000-fold reduction in inhibitory activity tested on papain. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | By similarityAdd BLAST | 26 | |
ChainiPRO_5000654409 | 27 – 141 | AsCystatinAdd BLAST | 115 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 91 ↔ 107 | By similarity | ||
Disulfide bondi | 120 ↔ 140 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PRIDEi | E3P6N3 |
Expressioni
Tissue specificityi
Expressed at a low level by the venom gland (at protein level).1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 129 | CystatinAdd BLAST | 101 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 73 – 77 | Secondary area of contactBy similarity | 5 |
Sequence similaritiesi
Belongs to the cystatin family.Curated
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00042 CY, 1 hit |
InterProi | View protein in InterPro IPR027214 Cystatin IPR000010 Cystatin_dom |
PANTHERi | PTHR11413 PTHR11413, 1 hit |
Pfami | View protein in Pfam PF00031 Cystatin, 1 hit |
SMARTi | View protein in SMART SM00043 CY, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
E3P6N3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVHSQLPVAA PLRLLCALLL LPLATMIPGG LSPRSVTDPD VQEAAEFAVQ
60 70 80 90 100
EYNALSANAY YYKQLRIVEA QSQVVTGAKY YLTMELMKTK CAKTTGKPKV
110 120 130 140
YKEIQNCELP PKAQQEKLTC RFQVWSCPWL QKIELTKMSC N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ411278 mRNA Translation: ACR83839.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ411278 mRNA Translation: ACR83839.1 |
3D structure databases
SMRi | E3P6N3 |
ModBasei | Search... |
MobiDBi | Search... |
Protein family/group databases
MEROPSi | I25.012 |
Proteomic databases
PRIDEi | E3P6N3 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Family and domain databases
CDDi | cd00042 CY, 1 hit |
InterProi | View protein in InterPro IPR027214 Cystatin IPR000010 Cystatin_dom |
PANTHERi | PTHR11413 PTHR11413, 1 hit |
Pfami | View protein in Pfam PF00031 Cystatin, 1 hit |
SMARTi | View protein in SMART SM00043 CY, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | CYT_AUSSU | |
Accessioni | E3P6N3Primary (citable) accession number: E3P6N3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 24, 2013 |
Last sequence update: | January 11, 2011 | |
Last modified: | May 23, 2018 | |
This is version 23 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |