UniProtKB - E2RM31 (E2RM31_CANLF)
Protein
Alpha-1,3-glucosyltransferase
Gene
ALG8
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Functioni
Catalytic activityi
- α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+ARBA annotationEC:2.4.1.265ARBA annotation
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.UniRule annotationARBA annotationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- alpha-1,3-mannosyltransferase activity Source: Ensembl
- dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: GO_Central
GO - Biological processi
- oligosaccharide-lipid intermediate biosynthetic process Source: GO_Central
- protein N-linked glycosylation Source: GO_Central
- protein N-linked glycosylation via asparagine Source: Ensembl
Keywordsi
Molecular function | GlycosyltransferaseUniRule annotation, Transferase |
Enzyme and pathway databases
Reactomei | R-CFA-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ALG8Imported |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris)Imported |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:37801, ALG8 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotationARBA annotation; Multi-pass membrane protein UniRule annotationARBA annotation
Other locations
- Membrane ARBA annotation; Multi-pass membrane protein ARBA annotation
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 141 – 158 | HelicalUniRule annotationAdd BLAST | 18 | |
Transmembranei | 188 – 214 | HelicalUniRule annotationAdd BLAST | 27 | |
Transmembranei | 234 – 259 | HelicalUniRule annotationAdd BLAST | 26 | |
Transmembranei | 335 – 355 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 361 – 378 | HelicalUniRule annotationAdd BLAST | 18 | |
Transmembranei | 429 – 449 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 461 – 483 | HelicalUniRule annotationAdd BLAST | 23 | |
Transmembranei | 489 – 508 | HelicalUniRule annotationAdd BLAST | 20 |
Keywords - Cellular componenti
Endoplasmic reticulumUniRule annotationARBA annotation, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_5003164238 | 19 – 526 | Alpha-1,3-glucosyltransferaseSequence analysisAdd BLAST | 508 |
Proteomic databases
PaxDbi | E2RM31 |
Expressioni
Gene expression databases
Bgeei | ENSCAFG00000004822, Expressed in pancreas and 50 other tissues |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalSequence analysis, Transmembrane, Transmembrane helixUniRule annotationARBA annotationPhylogenomic databases
eggNOGi | KOG2576, Eukaryota |
GeneTreei | ENSGT00940000153733 |
HOGENOMi | CLU_022045_2_0_1 |
InParanoidi | E2RM31 |
OMAi | DVHRNWL |
OrthoDBi | 595382at2759 |
TreeFami | TF315002 |
Family and domain databases
InterProi | View protein in InterPro IPR039487, ALG8 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF2, PTHR12413:SF2, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
E2RM31-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPGVAACG GNWFSALALG VTLLKCLLIP TYHSTDFEVH RNWLAITHSL
60 70 80 90 100
PISQWYYEAT SEWTLDYPPF FAWFEYALSH VAKYFDQEML NIHNLNYSSS
110 120 130 140 150
RTLLFQRFSV IFTDALFVYA VHECCKCIDG KKAGKELTEK PKFILSVLLL
160 170 180 190 200
WNFGLLIVDH IHFQYNGFLF GLMLLSIARL FQKRHMEGAL LFAVLLHFKH
210 220 230 240 250
IYLYVAPAYG IYLLRSYCFT ANKPDGSIRW NSFSFIRLIS LGLIVFLVSA
260 270 280 290 300
LSLGPFLALN QLPQVLSRLF PFKRGLCHAY WAPNFWALYN ALDKVLSVIG
310 320 330 340 350
LELKLLDPNK IPKASMTSGL VQQFQHTVLP SVTPLATLIC TLMAILPSVF
360 370 380 390 400
CLWFKPQGPR GFLRCLILCA LSSFLFGWHV HEKAILLAVL PMSLLSVGKA
410 420 430 440 450
GDASIFLILT TTGHYSLFPL LFTAPELPIK ILLMLLFTIY SISSLKTLFR
460 470 480 490 500
KEKTLFNWME TFYLLGLAPL EIFCEFVFPF TSWKLKYPFI PLLLTSVYCA
510 520
VGITYAWFRL YVSVLTDPPV GKTKKQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A5F4D7B0 | A0A5F4D7B0_CANLF | Alpha-1,3-glucosyltransferase | ALG8 | 242 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAEX03012784 Genomic DNA No translation available. AAEX03012785 Genomic DNA No translation available. |
RefSeqi | XP_533998.2, XM_533998.5 |
Genome annotation databases
Ensembli | ENSCAFT00000007783; ENSCAFP00000007215; ENSCAFG00000004822 |
GeneIDi | 476793 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAEX03012784 Genomic DNA No translation available. AAEX03012785 Genomic DNA No translation available. |
RefSeqi | XP_533998.2, XM_533998.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000007215 |
Proteomic databases
PaxDbi | E2RM31 |
Genome annotation databases
Ensembli | ENSCAFT00000007783; ENSCAFP00000007215; ENSCAFG00000004822 |
GeneIDi | 476793 |
Organism-specific databases
CTDi | 79053 |
VGNCi | VGNC:37801, ALG8 |
Phylogenomic databases
eggNOGi | KOG2576, Eukaryota |
GeneTreei | ENSGT00940000153733 |
HOGENOMi | CLU_022045_2_0_1 |
InParanoidi | E2RM31 |
OMAi | DVHRNWL |
OrthoDBi | 595382at2759 |
TreeFami | TF315002 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-CFA-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Gene expression databases
Bgeei | ENSCAFG00000004822, Expressed in pancreas and 50 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR039487, ALG8 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF2, PTHR12413:SF2, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | E2RM31_CANLF | |
Accessioni | E2RM31Primary (citable) accession number: E2RM31 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 30, 2010 |
Last sequence update: | November 30, 2010 | |
Last modified: | October 7, 2020 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |