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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) (Methanoplanus petrolearius)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADUniRule annotation1
Binding sitei63NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei127NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi156Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi171IronUniRule annotation1
Binding sitei219NAD; via amide nitrogenUniRule annotation1
Binding sitei229GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 56NADUniRule annotation2
Nucleotide bindingi154 – 156NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciMPET679926:G1GN5-114-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Mpet_0116Imported
OrganismiMethanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) (Methanoplanus petrolearius)Imported
Taxonomic identifieri679926 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanolacinia
Proteomesi
  • UP000006565 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi679926.Mpet_0116

Structurei

3D structure databases

ProteinModelPortaliE1RE05
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

E1RE05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDEVTISR AILSEQHKIM TEYLDIDCAV VGGGPSGITC AAILAQNGVK
60 70 80 90 100
VALIEKKLSI GGGMWGGGMM FPRIVVQEEA RRLLDHFGIK YTEYEKGYYV
110 120 130 140 150
ASSVEAVSKS LAAACDAGAE VFNLTTVEDV VVKEDGGVSG LVINWTAVEM
160 170 180 190 200
AGLHIDPLTM RTKVTVDATG HDSMIAHMVR KKGGALEIKG EGFMWAERAE
210 220 230 240 250
TNILSHTKEV FPGLIVAGMA ANAVGGETRM GPIFGGMLLS GEKAANMIIE

RLKK
Length:254
Mass (Da):27,073
Last modified:November 30, 2010 - v1
Checksum:iDFFB32F9BFC29590
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002117 Genomic DNA Translation: ADN34896.1
RefSeqiWP_013328075.1, NC_014507.1

Genome annotation databases

EnsemblBacteriaiADN34896; ADN34896; Mpet_0116
GeneIDi9742554
KEGGimpi:Mpet_0116

Similar proteinsi

Entry informationi

Entry nameiE1RE05_METP4
AccessioniPrimary (citable) accession number: E1RE05
Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: July 18, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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