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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Vulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ)
  2. no protein annotated in this organism
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34UniRule annotation1
Binding sitei37ATPUniRule annotation1
Binding sitei75ATPUniRule annotation1
Metal bindingi77Magnesium 1UniRule annotation1
Active sitei79Proton acceptorUniRule annotation1
Binding sitei100SubstrateUniRule annotation1
Metal bindingi101Magnesium 2UniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Metal bindingi250Magnesium 2UniRule annotation1
Binding sitei479ATPUniRule annotation1
Binding sitei516ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei519SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processPurine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciVDIS572478:G1GN6-585-MONOMER
UniPathwayi
UPA00074;UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:Vdis_0584Imported
OrganismiVulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017)Imported
Taxonomic identifieri572478 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaeVulcanisaeta
Proteomesi
  • UP000006681 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi572478.Vdis_0584

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 172AIRSInterPro annotationAdd BLAST101
Domaini185 – 331AIRS_CInterPro annotationAdd BLAST147
Domaini437 – 542AIRSInterPro annotationAdd BLAST106
Domaini560 – 692AIRS_CInterPro annotationAdd BLAST133

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 81Substrate bindingUniRule annotation4
Regioni293 – 295Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00641 Archaea
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG093Z00ZR

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 2 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit

Sequencei

Sequence statusi: Complete.

E1QUX8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLSDNEKSV IRKSLGREPT QAEWLVFEAE WSEHCSYKSS RRFLKLLPSG
60 70 80 90 100
APHVIRGPGL DAPLIRVGNL VVSFKIESHN HPSAVDPYDG AATGVGGIVR
110 120 130 140 150
DILTTGLRPI ALMDNLHFGP PNNPHSQWLM RNVVRGISDY GNRIGVPVVA
160 170 180 190 200
GEVWFDESFT TNPIVLVTCI GVGRPEDVVV GEASPGDIVL VIGNDVGRDG
210 220 230 240 250
MLGSSFASKI LERNEDNIGA VQVGNPLLEK LLIDALIELR GRGLVKAIKD
260 270 280 290 300
VGGGGLATAL SELADQFGLG IEVHLDRIRT REGMAPEEIL VSESQERMVI
310 320 330 340 350
VVSKEGLDEV EGILRRYGVG YDVVGVLTNN GMFVAYYGGE AIVNLPVKLI
360 370 380 390 400
THAPETNYDI REPHYLGELR AIPELPDVPL EEALTKILSD PNMASKEPIF
410 420 430 440 450
SLYDYEVGVR TVIKPGRAGA AVLRLLDVDG GDGKLGIAVK ADANPRYSFL
460 470 480 490 500
DPFIGAANSL AKAYRNVVAV GAEPLAAVDS INVGNPEKPD RYWYFVNSVL
510 520 530 540 550
GLAWFARELG IPVVGGKVSF YNEDSRGNVI KPVVAVAVLG RVDDVDEAVD
560 570 580 590 600
GGLIGDGALI IIGNTSNEMG GSEYLYAVHG IVRGIPPRPR PRDEVRNSRA
610 620 630 640 650
ILRLIHEGLV TASMDVGVGG LLTTLSKMAL INGVGFNIDL SRVPTDECGI
660 670 680 690 700
DPTALAFSET NARYVIEVRN KDVDRAMGIL SSLGVPFSVI GGSGGDHLVV
710 720
RWGHHELMSL KLNDLKTAYN SLWGVM
Length:726
Mass (Da):78,060
Last modified:November 30, 2010 - v1
Checksum:iB552981A832B39EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002100 Genomic DNA Translation: ADN49981.1
RefSeqiWP_013335706.1, NC_014537.1

Genome annotation databases

EnsemblBacteriaiADN49981; ADN49981; Vdis_0584
GeneIDi9751504
KEGGivdi:Vdis_0584

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002100 Genomic DNA Translation: ADN49981.1
RefSeqiWP_013335706.1, NC_014537.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi572478.Vdis_0584

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADN49981; ADN49981; Vdis_0584
GeneIDi9751504
KEGGivdi:Vdis_0584

Phylogenomic databases

eggNOGiarCOG00641 Archaea
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG093Z00ZR

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

BioCyciVDIS572478:G1GN6-585-MONOMER

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 2 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE1QUX8_VULDI
AccessioniPrimary (citable) accession number: E1QUX8
Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: November 7, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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