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Protein

ATP-dependent (S)-NAD(P)H-hydrate dehydratase

Gene

NAXD

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation

Catalytic activityi

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH.UniRule annotation
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH.

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135NAD(P)HX; via amide nitrogenUniRule annotation1
Binding sitei257NAD(P)HXUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi228 – 232ATPUniRule annotation5
Nucleotide bindingi247 – 256ATPUniRule annotation10

GO - Molecular functioni

Keywordsi

Molecular functionLyase
LigandATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-197264 Nicotinamide salvaging

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)
Alternative name(s):
ATP-dependent NAD(P)HX dehydrataseUniRule annotation
Carbohydrate kinase domain-containing proteinUniRule annotation
NAD(P)HX dehydrataseBy similarity
Gene namesi
Name:NAXDBy similarity
Synonyms:CARKDUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 12

Organism-specific databases

VGNCiVGNC:53829 NAXD

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionUniRule annotationAdd BLAST28
ChainiPRO_000041615629 – 329ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49N6-acetyllysineBy similarity1
Modified residuei67PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiE1BNQ4
PRIDEiE1BNQ4

Expressioni

Gene expression databases

BgeeiENSBTAG00000011466 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015238

Structurei

3D structure databases

SMRiE1BNQ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 326YjeF C-terminalUniRule annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 194NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrD/CARKD family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3974 Eukaryota
COG0063 LUCA
GeneTreeiENSGT00390000000917
InParanoidiE1BNQ4
KOiK17757
OMAiNLMVHPL
OrthoDBiEOG091G0CC8
TreeFamiTF300116

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
InterProiView protein in InterPro
IPR000631 CARKD
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
PROSITEiView protein in PROSITE
PS51383 YJEF_C_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E1BNQ4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALGPGCRAV RGCRPVLKRA FSLHKAHSVK DMESILQLVR SVVPALTTKK
60 70 80 90 100
HKGQDGRIGV VGGCREYTGA PYFAAISALK VGADLSHVFC TQEAAPVIKA
110 120 130 140 150
YSPELIVHPV LDSPEAVRDV EQWLPRLHAL VVGPGLGRDD ALLENVKGIL
160 170 180 190 200
EASKARGIPV VIDADGLWLI AQQPALIQGY RKAVLTPNHV EFGRLSEAVL
210 220 230 240 250
GVPLDGGDRH GAVLRLSQAL GNVTVVQKGE QDVISDGEQV LECSQEGSGR
260 270 280 290 300
RCGGQGDLLS GSLGVLAHWA LRAGPQKTGG PSPLLVAAFG ACALTRQCSQ
310 320
QAFQKYGRAT TTSDMVAEVG PAFRRLFEA
Length:329
Mass (Da):34,809
Last modified:November 2, 2010 - v1
Checksum:i7580A8A4A93374AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02034911 Genomic DNA No translation available.
DAAA02034912 Genomic DNA No translation available.
RefSeqiXP_002692037.1, XM_002691991.3

Genome annotation databases

EnsembliENSBTAT00000015238; ENSBTAP00000015238; ENSBTAG00000011466
GeneIDi613996
KEGGibta:613996

Similar proteinsi

Entry informationi

Entry nameiNNRD_BOVIN
AccessioniPrimary (citable) accession number: E1BNQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: November 2, 2010
Last modified: September 12, 2018
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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