UniProtKB - E1BLT8 (ZDHC5_BOVIN)
Palmitoyltransferase ZDHHC5
ZDHHC5
Functioni
Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates such as CTNND2, CD36, STAT3 and S1PR1 thus plays a role in various biological processes including cell adhesion, fatty acid uptake, bacterial sensing or cardiac functions. Plays an important role in the regulation of synapse efficacy by mediating palmitoylation of delta-catenin/CTNND2, thereby increasing synaptic delivery and surface stabilization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs). Under basal conditions, remains at the synaptic membrane through FYN-mediated phosphorylation that prevents association with endocytic proteins. Neuronal activity enhances the internalization and trafficking of DHHC5 from spines to dendritic shafts where it palmitoylates delta-catenin/CTNND2. Regulates cell adhesion at the plasma membrane by palmitoylating GOLGA7B and DSG2. Plays a role in innate immune response by mediating the palmitoylation of NOD1 and NOD2 and their proper recruitment to the bacterial entry site and phagosomes. Participates also in fatty acid uptake by palmitoylating CD36 and thereby targeting it to the plasma membrane. Upon binding of fatty acids to CD36, gets phosphorylated by LYN leading to inactivation and subsequent CD36 caveolar endocytosis.
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 134 | S-palmitoyl cysteine intermediateBy similarity | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: GO_Central
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- positive regulation of pattern recognition receptor signaling pathway Source: Ensembl
- positive regulation of protein localization to phagocytic vesicle Source: Ensembl
- positive regulation of protein localization to plasma membrane Source: Ensembl
- protein palmitoylation Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Immunity, Innate immunity, Lipid transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase ZDHHC5 (EC:2.3.1.225By similarity)Alternative name(s): Zinc finger DHHC domain-containing protein 5 Short name: DHHC-5 |
Gene namesi | Name:ZDHHC5 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000002381 |
VGNCi | VGNC:37143, ZDHHC5 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- dendrite Source: Ensembl
- integral component of membrane Source: UniProtKB-KW
- phagocytic vesicle Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 13 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 35 – 52 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 74 – 148 | CytoplasmicSequence analysisAdd BLAST | 75 | |
Transmembranei | 149 – 169 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 170 – 191 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 192 – 212 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 213 – 714 | CytoplasmicSequence analysisAdd BLAST | 502 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418363 | 1 – 714 | Palmitoyltransferase ZDHHC5Add BLAST | 714 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 247 | PhosphoserineBy similarity | 1 | |
Modified residuei | 294 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Modified residuei | 299 | PhosphoserineBy similarity | 1 | |
Modified residuei | 303 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 345 | PhosphoserineBy similarity | 1 | |
Modified residuei | 348 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 350 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 380 | PhosphoserineBy similarity | 1 | |
Modified residuei | 398 | PhosphoserineBy similarity | 1 | |
Modified residuei | 406 | PhosphoserineBy similarity | 1 | |
Modified residuei | 409 | PhosphoserineBy similarity | 1 | |
Modified residuei | 411 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 425 | PhosphoserineBy similarity | 1 | |
Modified residuei | 429 | PhosphoserineBy similarity | 1 | |
Modified residuei | 432 | PhosphoserineBy similarity | 1 | |
Modified residuei | 436 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 529 | PhosphoserineBy similarity | 1 | |
Modified residuei | 554 | PhosphoserineBy similarity | 1 | |
Modified residuei | 616 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 620 | PhosphoserineBy similarity | 1 | |
Modified residuei | 658 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 693 | PhosphoserineBy similarity | 1 | |
Modified residuei | 696 | Omega-N-methylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Palmitate, PhosphoproteinProteomic databases
PaxDbi | E1BLT8 |
PRIDEi | E1BLT8 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000002381, Expressed in retina and 96 other tissues |
Structurei
3D structure databases
AlphaFoldDBi | E1BLT8 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 154 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 289 – 714 | DisorderedSequence analysisAdd BLAST | 426 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 327 – 345 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 354 – 377 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 386 – 405 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 421 – 482 | Polar residuesSequence analysisAdd BLAST | 62 | |
Compositional biasi | 541 – 556 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 575 – 592 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 665 – 679 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156001 |
HOGENOMi | CLU_013779_0_0_1 |
InParanoidi | E1BLT8 |
OMAi | LAPRYMG |
OrthoDBi | 1264614at2759 |
TreeFami | TF354263 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPAESAKRFK PSKYVPVSAA AIFLVGATTL FFAFTCPGLS LSVSPAVPVY
60 70 80 90 100
NAVVFLFVLA NFSMATFMDP GVFPRAEEDE DKEDDFRAPL YKTVEIKGIQ
110 120 130 140 150
VRMKWCATCR FYRPPRCSHC SVCDNCVEEF DHHCPWVNNC IGRRNYRYFF
160 170 180 190 200
LFLLSLTAHI TGVFGFGLLY VLYHMEELSG VRTAVTMAVM CVAGLFFIPV
210 220 230 240 250
AGLTGFHVVL VARGRTTNEQ VTGKFRGGVN PFTNGCCNNV SRVLCSSPAP
260 270 280 290 300
RYLGRPKKEK TIVIRPPFLR PEVSDGQITV KIMDNGIQGE LRRTKSKGSL
310 320 330 340 350
EVTESQSADA EPPPPPKPDL SRYTGLRTHL SLATNEESSL LGKDSPPTPT
360 370 380 390 400
MYKYRPGYSS SSASAAMPHS SSAKLSRGDS LKEPPSIAES SRQPSYRSEP
410 420 430 440 450
SLEPESFRSP TLGKGFPFDP LSSGSRSSSL KSAQGTGFEL GPLQSIRSEG
460 470 480 490 500
TTSTSYKSLA NQTRNGSLSY DSLLTPSDSP DFESVQAGPE PEPPLGYTSP
510 520 530 540 550
FLSARLAQQR EAERHPRLAP GGPAPREPSP VRYDNLSRHI VASLQEREKL
560 570 580 590 600
LRQSPPPGRE EEPGLGDSGV QCTPGSGHAP RTSSSSDDSK RSPLGKTPLA
610 620 630 640 650
RPVAPRFGKP DGLRGRGLAS PEPGLPAPYL GRSVSYSSQK APPGVPETEE
660 670 680 690 700
VALQPLLTPK DEVQLKTAYS KSNGQPKSIG SAPPGPGQPP LSSPTRGGVK
710
KVSGVGGTTY EISV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02041769 Genomic DNA No translation available. |
RefSeqi | NP_001179692.1, NM_001192763.1 XP_005201016.1, XM_005200959.2 |
Genome annotation databases
Ensembli | ENSBTAT00000003078; ENSBTAP00000003078; ENSBTAG00000002381 |
GeneIDi | 533250 |
KEGGi | bta:533250 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02041769 Genomic DNA No translation available. |
RefSeqi | NP_001179692.1, NM_001192763.1 XP_005201016.1, XM_005200959.2 |
3D structure databases
AlphaFoldDBi | E1BLT8 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000003078 |
Proteomic databases
PaxDbi | E1BLT8 |
PRIDEi | E1BLT8 |
Genome annotation databases
Ensembli | ENSBTAT00000003078; ENSBTAP00000003078; ENSBTAG00000002381 |
GeneIDi | 533250 |
KEGGi | bta:533250 |
Organism-specific databases
CTDi | 25921 |
VEuPathDBi | HostDB:ENSBTAG00000002381 |
VGNCi | VGNC:37143, ZDHHC5 |
Phylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156001 |
HOGENOMi | CLU_013779_0_0_1 |
InParanoidi | E1BLT8 |
OMAi | LAPRYMG |
OrthoDBi | 1264614at2759 |
TreeFami | TF354263 |
Gene expression databases
Bgeei | ENSBTAG00000002381, Expressed in retina and 96 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDHC5_BOVIN | |
Accessioni | E1BLT8Primary (citable) accession number: E1BLT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2012 |
Last sequence update: | November 2, 2010 | |
Last modified: | May 25, 2022 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families