UniProtKB - E1BH29 (ALKB5_BOVIN)
Protein
RNA demethylase ALKBH5
Gene
ALKBH5
Organism
Bos taurus (Bovine)
Status
Functioni
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (By similarity). Can also demethylate N6-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export (By similarity). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity).By similarity
Catalytic activityi
- 2-oxoglutarate + N6-methyladenosine in mRNA + O2 = adenosine in mRNA + CO2 + formaldehyde + succinateBy similarity
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 204 | Iron; catalyticBy similarity | 1 | |
Metal bindingi | 206 | Iron; catalyticBy similarity | 1 | |
Metal bindingi | 266 | Iron; catalyticBy similarity | 1 | |
Binding sitei | 277 | Alpha-ketoglutarateBy similarity | 1 |
GO - Molecular functioni
- 2-oxoglutarate-dependent dioxygenase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- oxidative RNA demethylase activity Source: GO_Central
- RNA N6-methyladenosine dioxygenase activity Source: UniProtKB
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- mRNA export from nucleus Source: UniProtKB
- mRNA processing Source: UniProtKB
- oxidative single-stranded RNA demethylation Source: UniProtKB
- response to hypoxia Source: UniProtKB
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Differentiation, Spermatogenesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-BTA-73943, Reversal of alkylation damage by DNA dioxygenases |
Names & Taxonomyi
Protein namesi | Recommended name: RNA demethylase ALKBH5 (EC:1.14.11.-By similarity)Alternative name(s): Alkylated DNA repair protein alkB homolog 5 Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 |
Gene namesi | Name:ALKBH5 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus speckle By similarity
Nucleus
- nuclear speck Source: UniProtKB
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000421246 | 2 – 394 | RNA demethylase ALKBH5Add BLAST | 393 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 64 | PhosphoserineBy similarity | 1 | |
Modified residuei | 69 | PhosphoserineBy similarity | 1 | |
Modified residuei | 132 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 328 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 371 | PhosphoserineBy similarity | 1 | |
Modified residuei | 384 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | E1BH29 |
PRIDEi | E1BH29 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000025046, Expressed in spermatid and 18 other tissues |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000034857 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 193 – 195 | Alpha-ketoglutarate bindingBy similarity | 3 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 67 – 116 | Sequence analysisAdd BLAST | 50 |
Sequence similaritiesi
Belongs to the alkB family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4176, Eukaryota |
HOGENOMi | CLU_047472_1_0_1 |
InParanoidi | E1BH29 |
OMAi | ENYWRRD |
OrthoDBi | 722070at2759 |
TreeFami | TF329212 |
Family and domain databases
Gene3Di | 2.60.120.590, 1 hit |
InterProi | View protein in InterPro IPR027450, AlkB-like IPR037151, AlkB-like_sf IPR032860, ALKBH5 |
PANTHERi | PTHR32074, PTHR32074, 2 hits |
Pfami | View protein in Pfam PF13532, 2OG-FeII_Oxy_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
E1BH29-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAASGYTDL REKLKSMTSR DNYKAGSREA AAAAAAAVAA AAAAAAAAEP
60 70 80 90 100
YAAPGVKRKY PEDSDPERSD FEEQQLQKEE EARKVKSGIR QMRLFSQDEC
110 120 130 140 150
AKIEARIDEV VSRAEKGLYN EHTVDRAPLR NKYFFGEGYT YGAQLQKRGP
160 170 180 190 200
GQERLYPPGD VDEIPEWVHQ LVIQKLVEHR VIPEGFVNSA VINDYQPGGC
210 220 230 240 250
IVSHVDPIHI FERPIVSVSF FSDSALCFGC KFQFKPIRVS EPVLSLPVRR
260 270 280 290 300
GSVTVLSGYA ADEITHCIRP QDIKERRAVI ILRKTRLDAP RLETKSLSSS
310 320 330 340 350
VLPPSYASDR LSGNNRDPAL KPKRSHRKAD PDAAHRPRIL EMDKEENRRS
360 370 380 390
VLLPAHRRAG RFSSENYRRK SYEPGEDCSE AAGSPARKVK MRRH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02048990 Genomic DNA No translation available. |
RefSeqi | NP_001192446.1, NM_001205517.2 |
Genome annotation databases
GeneIDi | 533303 |
KEGGi | bta:533303 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02048990 Genomic DNA No translation available. |
RefSeqi | NP_001192446.1, NM_001205517.2 |
3D structure databases
SMRi | E1BH29 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000034857 |
Proteomic databases
PaxDbi | E1BH29 |
PRIDEi | E1BH29 |
Genome annotation databases
GeneIDi | 533303 |
KEGGi | bta:533303 |
Organism-specific databases
CTDi | 54890 |
Phylogenomic databases
eggNOGi | KOG4176, Eukaryota |
HOGENOMi | CLU_047472_1_0_1 |
InParanoidi | E1BH29 |
OMAi | ENYWRRD |
OrthoDBi | 722070at2759 |
TreeFami | TF329212 |
Enzyme and pathway databases
Reactomei | R-BTA-73943, Reversal of alkylation damage by DNA dioxygenases |
Gene expression databases
Bgeei | ENSBTAG00000025046, Expressed in spermatid and 18 other tissues |
Family and domain databases
Gene3Di | 2.60.120.590, 1 hit |
InterProi | View protein in InterPro IPR027450, AlkB-like IPR037151, AlkB-like_sf IPR032860, ALKBH5 |
PANTHERi | PTHR32074, PTHR32074, 2 hits |
Pfami | View protein in Pfam PF13532, 2OG-FeII_Oxy_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALKB5_BOVIN | |
Accessioni | E1BH29Primary (citable) accession number: E1BH29 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2013 |
Last sequence update: | November 2, 2010 | |
Last modified: | December 2, 2020 | |
This is version 64 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families