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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
  2. no protein annotated in this organism
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34UniRule annotation1
Binding sitei37ATPUniRule annotation1
Binding sitei76ATPUniRule annotation1
Metal bindingi78Magnesium 1UniRule annotation1
Active sitei80Proton acceptorUniRule annotation1
Binding sitei101SubstrateUniRule annotation1
Metal bindingi102Magnesium 2UniRule annotation1
Binding sitei224SubstrateUniRule annotation1
Metal bindingi252Magnesium 2UniRule annotation1
Binding sitei479ATPUniRule annotation1
Binding sitei516ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei519SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processPurine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:Igag_0264Imported
OrganismiIgnisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)Imported
Taxonomic identifieri583356 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeIgnisphaera
Proteomesi
  • UP000001304 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi583356.Igag_0264

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 174AIRSInterPro annotationAdd BLAST105
Domaini185 – 333AIRS_CInterPro annotationAdd BLAST149
Domaini423 – 540AIRSInterPro annotationAdd BLAST118
Domaini557 – 698AIRS_CInterPro annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 82Substrate bindingUniRule annotation4
Regioni295 – 297Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00641 Archaea
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG093Z00ZR

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 3 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit

Sequencei

Sequence statusi: Complete.

E0SQN9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLTNDEIEE IKRILGREPT VEELAMFEAQ WSEHCSYKSS RLLLRLLPTK
60 70 80 90 100
AKHVVLGPGR DAPAVEVFPD VLVVFKIESH NHPSAVDPYN GAATGIGGIV
110 120 130 140 150
RDILTLGAKP IALLDLLYMG NPRDPHANWL IRGIVKGISD YGNRIGIPTV
160 170 180 190 200
AGDTWFDEAF NKQPLVNVAC VGIVSPDKAV LSGPKSGDLI LIIGNATGRD
210 220 230 240 250
GLLGSSFASR PLAEDVDKDI GAVQVGDPLT EKLLIDVIQI LVEKNLVNYI
260 270 280 290 300
KDLGGGGLTT ALSESAADHG LGAVIYLDKL HTRQSLTPLE LLVSESQERM
310 320 330 340 350
LLAVPPTKIE DVKNILEGYE VPYSIIGYFD DSGIIKVFYM DRKVVEAPAK
360 370 380 390 400
ELAKPRAIRR PSKPPTEALK GFEPIILPDT EIDIEKTILL LLSSPNIASK
410 420 430 440 450
KWIYEQYDHE VGARTIIKPG YGDAAVLRLL DGSRRGIAVK GDANPRYTYL
460 470 480 490 500
DPFNGAANVV SECYRNLTAV GSTPLAIVDE LNAGNPEKPE HYWYFEEMLK
510 520 530 540 550
GLAWISDELG LPIVGGKVSF YNEDSRGVQV KPTLTVVGVG RIDDISYARG
560 570 580 590 600
MGFREYGSYI VVIGTTYPEI GGTEYLYRVH GIEHGEIPKP RPTYEISHGN
610 620 630 640 650
IVRRLIHRDI ALSVHDIGLG GLGIALIEMA IVSGIGFDVD LSVIPMRAVD
660 670 680 690 700
RLDVLLFSET QARYIIEIDG RRLDEAKKIL DESSVVYSVI GVTKGYEKTV
710 720 730
FRYNGNVVAS IDLINAVDIY MNSLERELEV V
Length:731
Mass (Da):80,109
Last modified:November 2, 2010 - v1
Checksum:i5B2D021AB497EA0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002098 Genomic DNA Translation: ADM27113.1

Genome annotation databases

EnsemblBacteriaiADM27113; ADM27113; Igag_0264
KEGGiiag:Igag_0264

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002098 Genomic DNA Translation: ADM27113.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi583356.Igag_0264

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADM27113; ADM27113; Igag_0264
KEGGiiag:Igag_0264

Phylogenomic databases

eggNOGiarCOG00641 Archaea
COG0046 LUCA
HOGENOMiHOG000238227
KOiK01952
OMAiFIEPYQG
OrthoDBiPOG093Z00ZR

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 3 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE0SQN9_IGNAA
AccessioniPrimary (citable) accession number: E0SQN9
Entry historyiIntegrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: February 28, 2018
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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