UniProtKB - D9J2T9 (D9J2T9_MOMBA)
Protein
rRNA N-glycosidase
Gene
RIP
Organism
Momordica balsamina (Bitter gourd) (Balsam apple)
Status
Functioni
Catalytic activityi
- Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.ARBA annotation EC:3.2.2.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 70 | FructoseCombined sources | 1 | |
Binding sitei | 85 | Beta-D-mannoseCombined sources | 1 | |
Binding sitei | 85 | CDPCombined sources | 1 | |
Binding sitei | 85 | CTPCombined sources | 1 | |
Binding sitei | 85 | UDPCombined sources | 1 | |
Binding sitei | 110 | Beta-D-mannose; via amide nitrogenCombined sources | 1 | |
Binding sitei | 111 | AscorbateCombined sources | 1 | |
Binding sitei | 122 | CTPCombined sources | 1 | |
Binding sitei | 163 | CDPCombined sources | 1 | |
Binding sitei | 163 | CTPCombined sources | 1 | |
Binding sitei | 163 | FructoseCombined sources | 1 | |
Binding sitei | 163 | UDPCombined sources | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 69 – 71 | UDPCombined sources | 3 | |
Nucleotide bindingi | 70 – 71 | CDPCombined sources | 2 | |
Nucleotide bindingi | 70 – 71 | CTPCombined sources | 2 | |
Nucleotide bindingi | 109 – 112 | CDPCombined sources | 4 | |
Nucleotide bindingi | 109 – 112 | CTPCombined sources | 4 | |
Nucleotide bindingi | 109 – 111 | UDPCombined sources | 3 |
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
- rRNA N-glycosylase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- negative regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | HydrolaseARBA annotation, Protein synthesis inhibitorARBA annotation, ToxinARBA annotation |
Ligand | Nucleotide-bindingCombined sources |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RIPImported |
Organismi | Momordica balsamina (Bitter gourd) (Balsam apple)Imported |
Taxonomic identifieri | 3672 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Cucurbitales › Cucurbitaceae › Momordiceae › Momordica |
PTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 227 | N-linked (GlcNAc...) asparagineCombined sources | 1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 71 | Ascorbate bindingCombined sources | 2 | |
Regioni | 109 – 111 | Fructose bindingCombined sources | 3 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
i Sequence
Sequence statusi: Fragment.
D9J2T9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
DVSFRLSGAD PSSYGMFIKD LRNALPHTEK VYNIPLLLPS VSGAGRYLLM
60 70 80 90 100
HLFNYDGNTI TVAVDVTNVY IMGYLALTTS YFFNEPAADL ASQYVFRSAR
110 120 130 140 150
RKITLPYSGN YERLQIAAGK PREKIPIGLP ALDTAISTLL HYDSTAAAGA
160 170 180 190 200
LLVLIQTTAE AARFKYIEQQ IQERAYRDEV PSSATISLEN SWSGLSKQIQ
210 220 230 240
LAQGNNGVFR TPTVLVDSKG NRVQITNVTS NVVTSNIQLL LNTKNI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | Imported | 1 | |
Non-terminal residuei | 246 | Imported | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM367595 mRNA Translation: ADK23804.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM367595 mRNA Translation: ADK23804.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3MRW | X-ray | 1.70 | A | 1-246 | [»] | |
3N1N | X-ray | 2.23 | A | 1-246 | [»] | |
3N2D | X-ray | 2.22 | A | 1-246 | [»] | |
3N31 | X-ray | 2.11 | A | 1-246 | [»] | |
3N3X | X-ray | 1.70 | A | 1-246 | [»] | |
3N5D | X-ray | 1.90 | A | 1-246 | [»] | |
3NFM | X-ray | 2.50 | A | 1-246 | [»] | |
3NJS | X-ray | 2.10 | A | 1-246 | [»] | |
3NX9 | X-ray | 1.70 | A | 1-246 | [»] | |
3Q4P | X-ray | 1.80 | A | 1-246 | [»] | |
3QJI | X-ray | 1.75 | A | 1-246 | [»] | |
3RL9 | X-ray | 1.90 | A | 1-246 | [»] | |
3S9Q | X-ray | 1.67 | A | 1-246 | [»] | |
3SJ6 | X-ray | 1.60 | A | 1-246 | [»] | |
3U6T | X-ray | 1.85 | A | 1-246 | [»] | |
3U6Z | X-ray | 1.70 | A | 1-246 | [»] | |
3U70 | X-ray | 2.00 | A | 1-246 | [»] | |
3U8F | X-ray | 1.80 | A | 1-246 | [»] | |
3V14 | X-ray | 1.70 | A | 1-246 | [»] | |
3V2K | X-ray | 2.07 | A | 1-246 | [»] | |
4DWM | X-ray | 1.62 | A | 1-246 | [»] | |
4EMF | X-ray | 1.77 | A | 1-246 | [»] | |
4EMR | X-ray | 1.75 | A | 1-246 | [»] | |
4F9N | X-ray | 2.65 | A | 1-246 | [»] | |
4FXA | X-ray | 1.70 | A | 1-246 | [»] | |
4FZ9 | X-ray | 1.70 | A | 1-246 | [»] | |
4GUW | X-ray | 1.60 | A | 1-246 | [»] | |
4H0Z | X-ray | 2.00 | A | 1-246 | [»] | |
4HOA | X-ray | 2.00 | A | 1-246 | [»] | |
4I47 | X-ray | 2.65 | A | 1-246 | [»] | |
4JTB | X-ray | 1.71 | A | 1-246 | [»] | |
4JTP | X-ray | 1.85 | A | 1-246 | [»] | |
4K2Z | X-ray | 1.80 | A | 1-246 | [»] | |
4KL4 | X-ray | 1.90 | A | 1-246 | [»] | |
4KMK | X-ray | 1.65 | A | 1-246 | [»] | |
4KPV | X-ray | 2.57 | A | 1-246 | [»] | |
4KWN | X-ray | 1.80 | A | 1-246 | [»] | |
4L66 | X-ray | 1.70 | A | 1-246 | [»] | |
4LRO | X-ray | 1.98 | A | 1-246 | [»] | |
4LT4 | X-ray | 1.69 | A | 1-246 | [»] | |
4LWX | X-ray | 1.78 | A | 1-246 | [»] | |
4M5A | X-ray | 1.70 | A | 1-246 | [»] | |
4O0O | X-ray | 2.59 | A | 1-246 | [»] | |
4O4Q | X-ray | 1.81 | A | 1-246 | [»] | |
4O8E | X-ray | 2.00 | A | 1-246 | [»] | |
4Q9F | X-ray | 1.75 | A | 1-246 | [»] | |
4RZJ | X-ray | 1.98 | A | 1-246 | [»] | |
4XY7 | X-ray | 2.50 | A | 1-246 | [»] | |
4ZT8 | X-ray | 1.98 | A | 1-246 | [»] | |
4ZU0 | X-ray | 1.80 | A | 1-246 | [»] | |
4ZZ6 | X-ray | 2.00 | A | 1-246 | [»] | |
5CIX | X-ray | 1.88 | A | 1-246 | [»] | |
5CSO | X-ray | 1.78 | A | 1-246 | [»] | |
5CST | X-ray | 1.78 | A | 1-246 | [»] | |
5GM7 | X-ray | 1.78 | A | 1-246 | [»] | |
5GZ7 | X-ray | 1.95 | A | 1-246 | [»] | |
5ILW | X-ray | 1.98 | A | 1-246 | [»] | |
5ILX | X-ray | 1.70 | A | 1-246 | [»] | |
5WV1 | X-ray | 1.90 | A | 1-246 | [»] | |
5Y48 | X-ray | 1.70 | A | 1-246 | [»] | |
SMRi | D9J2T9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Family and domain databases
Gene3Di | 3.40.420.10, 1 hit 4.10.470.10, 1 hit |
InterProi | View protein in InterPro IPR036041, Ribosome-inact_prot_sf IPR017989, Ribosome_inactivat_1/2 IPR001574, Ribosome_inactivat_prot IPR017988, Ribosome_inactivat_prot_CS IPR016138, Ribosome_inactivat_prot_sub1 IPR016139, Ribosome_inactivat_prot_sub2 |
PANTHERi | PTHR33453, PTHR33453, 1 hit |
Pfami | View protein in Pfam PF00161, RIP, 1 hit |
PRINTSi | PR00396, SHIGARICIN |
SUPFAMi | SSF56371, SSF56371, 1 hit |
PROSITEi | View protein in PROSITE PS00275, SHIGA_RICIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | D9J2T9_MOMBA | |
Accessioni | D9J2T9Primary (citable) accession number: D9J2T9 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 5, 2010 |
Last sequence update: | October 5, 2010 | |
Last modified: | April 7, 2021 | |
This is version 85 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |