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Entry version 51 (31 Jul 2019)
Sequence version 1 (05 Oct 2010)
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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Tibrogargan virus (strain CS132) (TIBV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.By similarity
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processmRNA capping, mRNA processing, Viral RNA replication, Viral transcription
LigandATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 4 domains:
RNA-directed RNA polymerase (EC:2.7.7.48By similarity)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56By similarity)
GDP polyribonucleotidyltransferase (EC:2.7.7.88By similarity)
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC:2.1.1.296By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:L
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiTibrogargan virus (strain CS132) (TIBV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1559361 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaNegarnaviricotaHaploviricotinaMonjiviricetesMononegaviralesRhabdoviridaeTibrovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiBos taurus (Bovine) [TaxID: 9913]
Culicoides brevitarsis [TaxID: 469753]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000029770 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Virion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004320521 – 2119RNA-directed RNA polymerase LAdd BLAST2119

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

May form homodimer.

Interacts with the P protein.

By similarity

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini612 – 798RdRp catalyticPROSITE-ProRule annotationAdd BLAST187
Domaini1658 – 1855Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST198

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the rhabdoviridae protein L family.Curated

Phylogenomic databases

KEGG Orthology (KO)

More...
KOi
K21316

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039530 L_methyltransferase_rhabdo
IPR039736 L_poly_C
IPR026890 Mononeg_mRNAcap
IPR014023 Mononeg_RNA_pol_cat
IPR025786 Mononega_L_MeTrfase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14314 Methyltrans_Mon, 1 hit
PF14318 Mononeg_mRNAcap, 1 hit
PF00946 Mononeg_RNA_pol, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR04198 paramyx_RNAcap, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50526 RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590 SAM_MT_MNV_L, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

D8V078-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDHNFEFYDD GGYDYDFSEE NVDDCIENVD CTDYCSLNLI NSYDYNLNSP
60 70 80 90 100
ITPEKLNNCL SYCQGLPYDQ IFYNPDYVKV KLMISHFKTV NVDDIPYYSE
110 120 130 140 150
LVKIWPKIIS NVNCPQKGRE FLVNAFDNFE FIYTIIRSFY KGWYKKEPET
160 170 180 190 200
TYLDVLTNLN RLLDRDLSWF SMFLDLFFII NLMNAKTVME QKNICLKRKW
210 220 230 240 250
KAYKLNDEII YFSGQTETLG VFALSGEFVL IESQNLLLDR NTTLMIKDTL
260 270 280 290 300
VGRFQTILSM ICLQNEYQYQ ENEISYLRQL YDLGDQILST YGSEGYDIIK
310 320 330 340 350
TLEMICNNWI CDESFKYFRP MPDFTSFRSH VTNTIQDLVN NGYALASAWF
360 370 380 390 400
NHIMNTTSIN LVLVFYGSFR HWGHPPIEVL QGLRNLEGLV NEKHDVDDNY
410 420 430 440 450
CQALASDLAF KVLKKKFRED KKWYVDHKKM DQNNLLYTHI KNNTWPNPQT
460 470 480 490 500
RIQFGDNWHK LPLIKCFELP DMIDLSSIYS DKSHSLTRSQ VVEHVRYNSD
510 520 530 540 550
KPIPTKRVLN TLLETENVNW PEFLKSVNDF GLPPEDLVIG LKPKEREMKR
560 570 580 590 600
TGRFFSLMTW NLRNYFVMTE LLIKEHFIGL FNGLTMADDL QGLIKKLLDR
610 620 630 640 650
TTGQGDSKIK KINIANGLDY TKWNNYQRYD SNRYVFRVMG QFLGYDMLIE
660 670 680 690 700
RTHQFFEQSL IYYPQRPDLM MVQNNTLENR GNEIVCWNGQ LGGLEGLRQK
710 720 730 740 750
GWSVLNYLMI ERESKVRNSQ IKILAQGDNQ IIFTSCFLDP YYSDEELLDN
760 770 780 790 800
MMRAKDNNEA IMNAVMKGAE KIGLVINMDE TIQSCCYANY GKVIIFRGKI
810 820 830 840 850
LGLSTKRWSR VTCSSNDQIP SLGTLLASVS TNAMTVGNFS ETPHDAILGH
860 870 880 890 900
LIFGLIVLEV LAQHNPAIRG DPSKYIVQHK LMSHPLFKII LLYLDPSLGG
910 920 930 940 950
IGGTSVNRFL IRAFADPVTE SLSFWKLIYE NCNDNVIKNL CLEVGNPSLA
960 970 980 990 1000
VYNDDHFLKL VEKPESLNIP KGISSTNMIK EQIKLSLINN AHNIKNKIIH
1010 1020 1030 1040 1050
DVTVRIREEE PALIAWLKSI KPVFPRFLSE MASSTFYGLS NNLISLFTNS
1060 1070 1080 1090 1100
RTIRNCFKTK CLKEVDYLII KSEVIGIVSC LKLVIKTHTN KPDNIWTCSA
1110 1120 1130 1140 1150
AHADKLRKQS WNEDIIGMTV PHPIEMHKIG YVVNGECLHC YENKLSQCYI
1160 1170 1180 1190 1200
SILTPRGIPN TDYYCEGGPY KPYLGSSTNE GTSVLQPWEK ETKIPIIRRA
1210 1220 1230 1240 1250
ARLRDVISWF VGEDSNLSES IINNLEALTG ENWGNYMRGF KRTGSALHRF
1260 1270 1280 1290 1300
RCARVSNGGF SACSPTKSSW MIVTTDTMTG LDESNYDFMF QASIIFSQVT
1310 1320 1330 1340 1350
VGSIQRSTSQ IYHMHLNCQE CLREIKEPLL ESDWVLKPRP VHELLKQWRP
1360 1370 1380 1390 1400
DPDLPWGFKN QICEIKNHST EWDLEPNTSK CYFVGLILGF LFGDKVLSNG
1410 1420 1430 1440 1450
IQTENNLFPL SIRSRLDPSY FYGGLLRGFK LIAAIHLTHR RNVISGQDSK
1460 1470 1480 1490 1500
SMLYGTLYYL IEEVSCDTDF VQFVSTGNLH NELFYSPHKI PPSYPLSGKD
1510 1520 1530 1540 1550
LGSLCRSYLK YQLKDSDMSS ELRYVWVFAD IRSPKLLCTL GISLYVERLL
1560 1570 1580 1590 1600
FKESLTKKDK ELIKRYQEDY ILGNNNELPT DMLNFYISNL RFCESEVRHS
1610 1620 1630 1640 1650
CKFSLPKIEY SLNQATSTWG QECWGYVNEL DIICVADTIS YPKIQVPQYR
1660 1670 1680 1690 1700
NPVISGLRLF QCATGAHYKI RSILKHYSVQ YRDALIGGDG SGGISALCLR
1710 1720 1730 1740 1750
YNARSRIIFN SLLQLEDSIL SGSRPTGPSA VASMGDMKLR CVNFDSVWQE
1760 1770 1780 1790 1800
PSDLRELQTW KYFLRLKQDF NLKIDLAIFD MEVTDEISIS QIDDMLISHL
1810 1820 1830 1840 1850
HCIFTTQTNT LIYKTYLDRV LNYPDMLLKL CQNFKSVKAV TTEFSSFKTS
1860 1870 1880 1890 1900
EIYLVCQNIE TYPILSRTAL EEETRQRLLK LAYANSPLSH EISRAFKIYS
1910 1920 1930 1940 1950
RKDLMDGVPN HLISDPFLDL STLFVMSGSL TSDACAILQL KNRSNLLNIL
1960 1970 1980 1990 2000
ISELCKLTNI IFELTVIHPK KIKVPSSPNI NNYFALVIGI GTWFAFVLND
2010 2020 2030 2040 2050
PDYIRLIDAI INKQVYTDIY FYAQKNNKGI INQWKVSDNQ SGKLICKKKF
2060 2070 2080 2090 2100
HISHKLATIG QVIRSCELSY RTITNPLPIQ STSVNQLIQQ KNKKLKLKYI
2110
FKSCDLFFYL PDSILDTST
Length:2,119
Mass (Da):244,351
Last modified:October 5, 2010 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB01916DDEC636B3D
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
GQ294472 Viral cRNA Translation: ADG86355.1

NCBI Reference Sequences

More...
RefSeqi
YP_007641376.1, NC_020804.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
14857905

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vg:14857905

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ294472 Viral cRNA Translation: ADG86355.1
RefSeqiYP_007641376.1, NC_020804.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi14857905
KEGGivg:14857905

Phylogenomic databases

KOiK21316

Family and domain databases

InterProiView protein in InterPro
IPR039530 L_methyltransferase_rhabdo
IPR039736 L_poly_C
IPR026890 Mononeg_mRNAcap
IPR014023 Mononeg_RNA_pol_cat
IPR025786 Mononega_L_MeTrfase
PfamiView protein in Pfam
PF14314 Methyltrans_Mon, 1 hit
PF14318 Mononeg_mRNAcap, 1 hit
PF00946 Mononeg_RNA_pol, 1 hit
TIGRFAMsiTIGR04198 paramyx_RNAcap, 1 hit
PROSITEiView protein in PROSITE
PS50526 RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590 SAM_MT_MNV_L, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiL_TIBVC
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: D8V078
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: October 5, 2010
Last modified: July 31, 2019
This is version 51 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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