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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Nitrospira defluvii
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42NADUniRule annotation1
Binding sitei69NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei135NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi167Iron; shared with adjacent protomerUniRule annotation1
Binding sitei167NAD; shared with adjacent protomerUniRule annotation1
Metal bindingi182IronUniRule annotation1
Binding sitei230NAD; via amide nitrogenUniRule annotation1
Binding sitei240GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 62NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciCNIT330214:NIDE_RS04900-MONOMER
UniPathwayi
UPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
ORF Names:NIDE1062Imported
OrganismiNitrospira defluviiImported
Taxonomic identifieri330214 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeNitrospira
Proteomesi
  • UP000001660 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi330214.NIDE1062

Structurei

3D structure databases

ProteinModelPortaliD8PC63
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IQJ Bacteria
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OrthoDBiPOG091H09WG

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

D8PC63-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKPKPAPLR ERDITRQIAR EYYKEFDQLI ESDVIIVGAG PSGLICAHDL
60 70 80 90 100
GRMGIKTLIV EQSLALGGGF WSGGYLMNKA TICAPAHKIL KEVGVPCKQI
110 120 130 140 150
KECPGMYMVD PPHATGALIA AAYNAGAKIM NLTRVVDLIL RREGVLEGVV
160 170 180 190 200
VNSTTAEMAG HDIIHVDPIA LESKIVVDAT GHDAVVVNLL HKRGLYQQVP
210 220 230 240 250
GNGAMWVSRS EEEVMDRTGE VSPNCFVIGL AVAAVFGTPR MGPAFGSMLL
260
SGRYGAELIR DKLKNR
Length:266
Mass (Da):28,628
Last modified:October 5, 2010 - v1
Checksum:i8C78784F903340C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP929003 Genomic DNA Translation: CBK40822.1
RefSeqiWP_013247648.1, NC_014355.1

Genome annotation databases

EnsemblBacteriaiCBK40822; CBK40822; NIDE1062
KEGGinde:NIDE1062

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP929003 Genomic DNA Translation: CBK40822.1
RefSeqiWP_013247648.1, NC_014355.1

3D structure databases

ProteinModelPortaliD8PC63
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330214.NIDE1062

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBK40822; CBK40822; NIDE1062
KEGGinde:NIDE1062

Phylogenomic databases

eggNOGiENOG4105IQJ Bacteria
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OrthoDBiPOG091H09WG

Enzyme and pathway databases

UniPathwayi
UPA00060

BioCyciCNIT330214:NIDE_RS04900-MONOMER

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiD8PC63_9BACT
AccessioniPrimary (citable) accession number: D8PC63
Entry historyiIntegrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: July 18, 2018
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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