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Protein

Megakaryocyte and platelet inhibitory receptor G6b

Gene

Mpig6b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitory receptor that acts as a critical regulator of hematopoietic lineage differentiation, megakaryocyte function and platelet production (PubMed:23112346). Inhibits platelet aggregation and activation by agonists such as ADP and collagen-related peptide (By similarity). This regulation of megakaryocate function as well as platelet production ann activation is done through the inhibition (via the 2 ITIM motifs) of the receptors CLEC1B and GP6:FcRgamma signaling (PubMed:23112346). Appears to operate in a calcium-independent manner (By similarity).By similarity1 Publication

GO - Biological processi

Keywordsi

Molecular functionReceptor

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Megakaryocyte and platelet inhibitory receptor G6bBy similarity
Alternative name(s):
ITIM-receptor G6b-B1 Publication
Immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing platelet receptor1 Publication
Gene namesi
Name:Mpig6bBy similarity
Synonyms:AU023871Imported, G6bImported, G6b-B1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146995 Mpig6b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice exhibit macrothrombocytopenia (reduced platelet numbers and the presence of enlarged platelets) and a susceptibility to bleeding because of defective platelet production and function. They show an increased platelet turnover leading to reduced platelet numbers. Their megakaryocytes exhibit reduced integrin-mediated functions, defective formation of proplatelets as well as an increased metalloproteinase production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_500968921618 – 242Megakaryocyte and platelet inhibitory receptor G6bAdd BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi112N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei212PhosphotyrosineBy similarity1

Post-translational modificationi

N-glycosylated.1 Publication
May be O-glycosylated.By similarity
Phosphorylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiD7PDD4
PaxDbiD7PDD4
PRIDEiD7PDD4

PTM databases

iPTMnetiD7PDD4
PhosphoSitePlusiD7PDD4

Expressioni

Tissue specificityi

Espressed in mature megakaryocytes and platelets. Not expressed by immature megakaryocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000073414 Expressed in 19 organ(s), highest expression level in bone marrow
ExpressionAtlasiD7PDD4 baseline and differential

Interactioni

Subunit structurei

Interacts (via ITIM motif) with PTPN6 and PTPN11 (PubMed:23112346). Binds to heparin (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133377

Structurei

3D structure databases

ProteinModelPortaliD7PDD4
SMRiD7PDD4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi210 – 215ITIM motif1 Publication6
Motifi236 – 241ITIM motif1 Publication6

Domaini

Contains both a transmembrane region and 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. The 2 ITIM motifs of isoform B are required for the inhibition of CLEC1B and GP6:FCER1G signaling and platelet activation.1 Publication

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYA7 Eukaryota
ENOG41118MY LUCA
GeneTreeiENSGT00390000017793
InParanoidiD7PDD4
OMAiPRFPACK
OrthoDBiEOG091G0N3N

Family and domain databases

InterProiView protein in InterPro
IPR028070 G6B
PANTHERiPTHR37347 PTHR37347, 1 hit
PfamiView protein in Pfam
PF15096 G6B, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

D7PDD4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALVLPLLPL LLSKVQGNPE VSLEGSPGDR VNLSCIGVSD PTRWAWAPSF
60 70 80 90 100
PACKGLSKGR RPILWASTRG TPTVLQHFSG RLRSLDNGIK RLELLLSAGD
110 120 130 140 150
SGTFFCKGRH ENESRTVLQV LGDKAGCRPA GSTHGYEYPK VLIPLLGVGL
160 170 180 190 200
VLGLGVAGVV WRRRRLSPPP PPPPPPGPLP TFAPVINAEP QRPLEQESKI
210 220 230 240
SGHLDQEPSL HYADLDHSVL GRHRRMSTVV SGDASTVYAV VV
Length:242
Mass (Da):26,010
Last modified:August 10, 2010 - v1
Checksum:i99FDE8AEA82A5F9F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YVS6D3YVS6_MOUSE
Megakaryocyte and platelet inhibito...
Mpig6b G6b
197Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087117 Genomic DNA No translation available.
GU269270 mRNA Translation: ADD91692.1
CCDSiCCDS57071.1
RefSeqiNP_001177941.1, NM_001191012.1
UniGeneiMm.390956

Genome annotation databases

EnsembliENSMUST00000174190; ENSMUSP00000133377; ENSMUSG00000073414
GeneIDi106722
KEGGimmu:106722
UCSCiuc012aqy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC087117 Genomic DNA No translation available.
GU269270 mRNA Translation: ADD91692.1
CCDSiCCDS57071.1
RefSeqiNP_001177941.1, NM_001191012.1
UniGeneiMm.390956

3D structure databases

ProteinModelPortaliD7PDD4
SMRiD7PDD4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133377

PTM databases

iPTMnetiD7PDD4
PhosphoSitePlusiD7PDD4

Proteomic databases

MaxQBiD7PDD4
PaxDbiD7PDD4
PRIDEiD7PDD4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000174190; ENSMUSP00000133377; ENSMUSG00000073414
GeneIDi106722
KEGGimmu:106722
UCSCiuc012aqy.1 mouse

Organism-specific databases

CTDi80739
MGIiMGI:2146995 Mpig6b

Phylogenomic databases

eggNOGiENOG410IYA7 Eukaryota
ENOG41118MY LUCA
GeneTreeiENSGT00390000017793
InParanoidiD7PDD4
OMAiPRFPACK
OrthoDBiEOG091G0N3N

Enzyme and pathway databases

ReactomeiR-MMU-114604 GPVI-mediated activation cascade

Miscellaneous databases

PROiPR:D7PDD4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073414 Expressed in 19 organ(s), highest expression level in bone marrow
ExpressionAtlasiD7PDD4 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR028070 G6B
PANTHERiPTHR37347 PTHR37347, 1 hit
PfamiView protein in Pfam
PF15096 G6B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG6B_MOUSE
AccessioniPrimary (citable) accession number: D7PDD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2017
Last sequence update: August 10, 2010
Last modified: November 7, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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