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Entry version 56 (02 Jun 2021)
Sequence version 1 (10 Aug 2010)
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Protein

Glucosylglycerate phosphorylase

Gene

Mesil_0665

Organism
Meiothermus silvanus (strain ATCC 700542 / DSM 9946 / VI-R2) (Thermus silvanus)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible phosphorolysis of glucosylglycerate into alpha-D-glucose 1-phosphate (Glc1P) and D-glycerate. May be a regulator of intracellular levels of glucosylglycerate, a compatible solute that primarily protects organisms facing salt stress and very specific nutritional constraints. Has a very strict substrate specificity. Cannot catalyze the phosphorolysis of sucrose or synthesize sucrose from Glc1P and D-fructose.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.83 sec(-1) towards 2-O-(alpha-D-glucopyranosyl)-D-glycerate for the phosphorolytic direction (at pH 6.0 and 42 degrees Celsius). kcat is 0.98 sec(-1) towards phosphate for the phosphorolytic direction (at pH 6.0 and 42 degrees Celsius). kcat is 96 sec(-1) towards alpha-D-glucose 1-phosphate for the synthetic direction (at pH 6.5 and 42 degrees Celsius). kcat is 128 sec(-1) towards D-glycerate for the synthetic direction (at pH 6.5 and 42 degrees Celsius).1 Publication
  1. KM=3.5 mM for 2-O-(alpha-D-glucopyranosyl)-D-glycerate (at pH 6.0 and 42 degrees Celsius)1 Publication
  2. KM=2.5 mM for phosphate (at pH 6.0 and 42 degrees Celsius)1 Publication
  3. KM=8.1 mM for alpha-D-glucose 1-phosphate (at pH 6.5 and 42 degrees Celsius)1 Publication
  4. KM=2.6 mM for D-glycerate (at pH 6.5 and 42 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6 and 6.5 in phosphorolytic and synthetic directions, respectively.1 Publication

    Temperature dependencei

    Optimum temperature is 42 degrees Celsius. Retains full activity after incubating at up to 55 degrees Celsius for 10 minutes, but only 35% is left after incubating at 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei231NucleophileBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MSIL526227:G1GKK-676-MONOMER

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    2.4.1.352, 15676

    Protein family/group databases

    Carbohydrate-Active enZymes

    More...
    CAZyi
    GH13, Glycoside Hydrolase Family 13

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Glucosylglycerate phosphorylase1 Publication (EC:2.4.1.3521 Publication)
    Short name:
    GGa phosphorylase1 Publication
    Short name:
    GGaP1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Ordered Locus Names:Mesil_0665Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMeiothermus silvanus (strain ATCC 700542 / DSM 9946 / VI-R2) (Thermus silvanus)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri526227 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMeiothermus
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001916 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

    The excellent specific activity of this enzyme towards glycerate opens up new options for the commercial production of glucosylglycerate. This compound has been reported to be an exceptionally potent and versatile stabilizer of proteins at elevated temperatures.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi275N → A: 75-fold decrease in catalytic activity. 1 Publication1
    Mutagenesisi275N → H: 38-fold decrease in catalytic activity. 1 Publication1
    Mutagenesisi383E → A or Q: More than 100-fold decrease in catalytic activity. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004424331 – 555Glucosylglycerate phosphorylaseAdd BLAST555

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    526227.Mesil_0665

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG0366, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_021358_0_0_0

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    NFTASHD

    Database of Orthologous Groups

    More...
    OrthoDBi
    1573900at2

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd11356, AmyAc_Sucrose_phosphorylase-like_1, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR033746, GGa_phosphorylase
    IPR006047, Glyco_hydro_13_cat_dom
    IPR017853, Glycoside_hydrolase_SF
    IPR016377, Sucrose_GGa_phosphorylase-rel

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00128, Alpha-amylase, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF003059, Sucrose_phosphorylase, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00642, Aamy, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51445, SSF51445, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    D7BAR0-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSSLTPELRQ SILEHLGFLY GERAPAVLGR LEEICSGFPA QRREGGWSEK
    60 70 80 90 100
    DALLITYGDQ IHAEGEPPLQ TLYDFLYERL RGVFSGVHLL PFYPSTSDDG
    110 120 130 140 150
    FSVVDFQRVD PELGTWTDIR IIAQDFRLMA DLVCNHVSAS SPWFQGFLQD
    160 170 180 190 200
    DPQYQGFFIT VDPGTDLSTV FRPRALPLLT PFQTPSGEKL VWTTFSPDQT
    210 220 230 240 250
    DLNYANPEVL LEVIEALLCY VRNGAGLIRL DAVGFIWKEI GTSCMHLEGA
    260 270 280 290 300
    HRIVKLMRLV LDAVAPHVLL VSETNAPHRE NISYFGNGHD EAQLVYQFPL
    310 320 330 340 350
    PPLVMHTFRT GDASKLAGWA AGLTLPSERT TFFNFLASHD GIGVVPAGGI
    360 370 380 390 400
    LQPEEIAALV RQALEHGGRV NHKDTPDGPV PYELCLTLFD ALSNPNSDEA
    410 420 430 440 450
    EDLKIARFLA ANVILLSLQG IPGVYIHSLF GSPSDHAGFE ESGIPRRLNR
    460 470 480 490 500
    HKFTKAELEE RLADPASRAA KILAAYSHLL RVRSMHPAFH PNAPQRILPS
    510 520 530 540 550
    TEVLRIVRGE GDQAVGCYIN VTDRPQVVSR IGKNLITGQW FTGVLKPYQA

    AWIID
    Length:555
    Mass (Da):61,531
    Last modified:August 10, 2010 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6EFA0A04A23A3E5B
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    CP002042 Genomic DNA Translation: ADH62582.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_013157171.1, NC_014212.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    ADH62582; ADH62582; Mesil_0665

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    msv:Mesil_0665

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP002042 Genomic DNA Translation: ADH62582.1
    RefSeqiWP_013157171.1, NC_014212.1

    3D structure databases

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    SWISS-MODEL Interactive Workspace

    More...
    SWISS-MODEL-Workspacei
    Submit a new modelling project...

    Protein-protein interaction databases

    STRINGi526227.Mesil_0665

    Protein family/group databases

    CAZyiGH13, Glycoside Hydrolase Family 13

    Genome annotation databases

    EnsemblBacteriaiADH62582; ADH62582; Mesil_0665
    KEGGimsv:Mesil_0665

    Phylogenomic databases

    eggNOGiCOG0366, Bacteria
    HOGENOMiCLU_021358_0_0_0
    OMAiNFTASHD
    OrthoDBi1573900at2

    Enzyme and pathway databases

    BioCyciMSIL526227:G1GKK-676-MONOMER
    BRENDAi2.4.1.352, 15676

    Family and domain databases

    CDDicd11356, AmyAc_Sucrose_phosphorylase-like_1, 1 hit
    InterProiView protein in InterPro
    IPR033746, GGa_phosphorylase
    IPR006047, Glyco_hydro_13_cat_dom
    IPR017853, Glycoside_hydrolase_SF
    IPR016377, Sucrose_GGa_phosphorylase-rel
    PfamiView protein in Pfam
    PF00128, Alpha-amylase, 1 hit
    PIRSFiPIRSF003059, Sucrose_phosphorylase, 1 hit
    SMARTiView protein in SMART
    SM00642, Aamy, 1 hit
    SUPFAMiSSF51445, SSF51445, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGGAP_MEISD
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: D7BAR0
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2017
    Last sequence update: August 10, 2010
    Last modified: June 2, 2021
    This is version 56 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
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