UniProtKB - D7AF63 (SFRA_GEOSK)
Protein
NADPH-Fe(3+) oxidoreductase subunit alpha
Gene
sfrA
Organism
Geobacter sulfurreducens (strain DL-1 / KN400)
Status
Functioni
The SfrAB enzymatic complex is probably involved in acetate metabolism and does not participate directly in the reduction of Fe3+ chelates. May serve as a major route for NADP regeneration.1 Publication
Cofactori
[4Fe-4S] clusterPROSITE-ProRule annotation
Activity regulationi
Not regulated by FAD or FMN.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 45 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 62 | Iron-sulfur 1 (2Fe-2S)By similarity | 1 | |
Metal bindingi | 94 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 98 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 101 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 107 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 146 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 149 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 152 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 186 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 189 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 192 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 196 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 222 | Iron-sulfur 5 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 225 | Iron-sulfur 5 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 229 | Iron-sulfur 5 (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 256 | Iron-sulfur 5 (4Fe-4S)Sequence analysis | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- molybdopterin cofactor binding Source: InterPro
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
GO - Biological processi
- ATP synthesis coupled electron transport Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | GSUL663917:G1GWJ-505-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH-Fe(3+) oxidoreductase subunit alpha (EC:1.-.-.-)Alternative name(s): Soluble Fe(3+) reductase alpha subunit |
Gene namesi | Name:sfrA Ordered Locus Names:KN400_0498 |
Organismi | Geobacter sulfurreducens (strain DL-1 / KN400) |
Taxonomic identifieri | 663917 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter › |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
SfrAB-null strain is unable to grow in the presence of acetate and fumarate unless an additional electron donor is present. Strongly reduced NADPH-dependent benzyl viologen reductase activity.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000429034 | 1 – 844 | NADPH-Fe(3+) oxidoreductase subunit alphaAdd BLAST | 844 |
Interactioni
Subunit structurei
Heterotetramer with 2 beta subunits.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 78 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 78 – 117 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 137 – 168 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 32 | |
Domaini | 177 – 206 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 215 – 270 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 56 |
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | CLU_000422_4_0_7 |
OMAi | QDQAMAY |
OrthoDBi | 323168at2 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF13237, Fer4_10, 1 hit PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 2 hits PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit |
i Sequence
Sequence statusi: Complete.
D7AF63-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSLTIDGKD ITVAKETTIL DAAALLGITI PTLCWLKKVS PTGACRVCAV
60 70 80 90 100
EIEGVDRPMT ACNTPVKDGI KVTTQSEKLS RIRQKIMELM LVNHPLDCPV
110 120 130 140 150
CDAGGECDLQ NACYGLGAAK QEYGAVLERR KIRYDWPLIE SDPNRCILCE
160 170 180 190 200
KCVKVDHEIV GCNAIRVVNR GEATIIDTVD GNPLNCEFCG NCVAACPTGT
210 220 230 240 250
LISKPFKFRG RPWAFTTTPS VCPFCATGCQ IEYHSRNGRV ERVTSDDSTY
260 270 280 290 300
NSGNLCINGR FGYSYINSPD RLAEPMVKGQ KADWNTAMGT AATALKQIVA
310 320 330 340 350
SHGADAVAGF GSPRVTNEDN YLFQKLMRSA IGTGNIDSEA RLGFAATQKV
360 370 380 390 400
LREMLGIAGA STTIDAIDRA TAVLVVGCDL NAEATGMEYR VIKAATKNNA
410 420 430 440 450
KLVLAAMRDI KLKKFANSHL KYRPGNETLL INALTKAVLE EGLENKEFCS
460 470 480 490 500
ANISNLSDLT AALAGVSIAD AAAATGVTEA DLRAAARLVG GKKGVAVIFG
510 520 530 540 550
AELMRGGNTD AVKALINLAL ILGATAGDTG GLFPVYEKTN IRGLLDMGVA
560 570 580 590 600
PDHFPGHQTD GTTFEKAWGK KLPAAAGKDL WQIIEGIEQG SVKALYLLGC
610 620 630 640 650
DPVASFPEGE RIRKALEKLE LLIVQDPFPG EAAKMAHVVF PSSVAAEKNG
660 670 680 690 700
TFTTIDGRVQ PLAKAVAPSG DAREDWDILT ELYNRLTGES RIHSPAAVLD
710 720 730 740 750
EVAALVPAYA SVGRTGGTIT AQPRSGGLAL APVSARAVAG SPTTLLVGTI
760 770 780 790 800
LYHSGTTTTW SKNNLEIIPK GYIEIHPNDA AKLGIAEGGK VRLSAGSVKV
810 820 830 840
EGTAKITPRV QPGLLFAPSH FRGMNVNALL SRDGGVVPVT VEKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002031 Genomic DNA Translation: ADI83361.1 |
RefSeqi | WP_010941177.1, NC_017454.1 |
Genome annotation databases
KEGGi | gsk:KN400_0498 |
PATRICi | fig|663917.3.peg.505 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002031 Genomic DNA Translation: ADI83361.1 |
RefSeqi | WP_010941177.1, NC_017454.1 |
3D structure databases
SMRi | D7AF63 |
ModBasei | Search... |
Genome annotation databases
KEGGi | gsk:KN400_0498 |
PATRICi | fig|663917.3.peg.505 |
Phylogenomic databases
HOGENOMi | CLU_000422_4_0_7 |
OMAi | QDQAMAY |
OrthoDBi | 323168at2 |
Enzyme and pathway databases
BioCyci | GSUL663917:G1GWJ-505-MONOMER |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd |
Pfami | View protein in Pfam PF13237, Fer4_10, 1 hit PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF54292, SSF54292, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 2 hits PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SFRA_GEOSK | |
Accessioni | D7AF63Primary (citable) accession number: D7AF63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 14, 2014 |
Last sequence update: | August 10, 2010 | |
Last modified: | April 7, 2021 | |
This is version 54 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |