UniProtKB - D6ZGP3 (D6ZGP3_MOBCV)
Protein
3-isopropylmalate dehydrogenase
Gene
leuB
Organism
Mobiluncus curtisii (strain ATCC 43063 / DSM 2711 / V125) (Falcivibrio vaginalis)
Status
Functioni
Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.UniRule annotation
Catalytic activityi
- EC:1.1.1.85UniRule annotation
Cofactori
Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation
: L-leucine biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- 2-isopropylmalate synthase (leuA)
- 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
- 3-isopropylmalate dehydrogenase (leuB)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 94 | SubstrateUniRule annotation | 1 | |
Binding sitei | 104 | SubstrateUniRule annotation | 1 | |
Binding sitei | 128 | SubstrateUniRule annotation | 1 | |
Sitei | 135 | Important for catalysisUniRule annotation | 1 | |
Sitei | 185 | Important for catalysisUniRule annotation | 1 | |
Metal bindingi | 218 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 218 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 242 | Magnesium or manganeseUniRule annotation | 1 | |
Metal bindingi | 246 | Magnesium or manganeseUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 278 – 290 | NADUniRule annotationAdd BLAST | 13 |
GO - Molecular functioni
- 3-isopropylmalate dehydrogenase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: InterPro
- NAD binding Source: InterPro
GO - Biological processi
- leucine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | OxidoreductaseUniRule annotation |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisUniRule annotation |
Ligand | MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation |
Enzyme and pathway databases
BioCyci | GCF_000196535-HMP:HMPREF0573_RS07245-MONOMER |
UniPathwayi | UPA00048;UER00072 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-isopropylmalate dehydrogenaseUniRule annotation (EC:1.1.1.85UniRule annotation)Alternative name(s): 3-IPM-DHUniRule annotation Beta-IPM dehydrogenaseUniRule annotation Short name: IMDHUniRule annotation |
Gene namesi | Name:leuBUniRule annotation Ordered Locus Names:HMPREF0573_11482Imported |
Organismi | Mobiluncus curtisii (strain ATCC 43063 / DSM 2711 / V125) (Falcivibrio vaginalis)Imported |
Taxonomic identifieri | 548479 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Actinomycetales › Actinomycetaceae › Mobiluncus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 548479.HMPREF0573_11482 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 346 | Iso_dhInterPro annotationAdd BLAST | 342 |
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.UniRule annotation
Phylogenomic databases
HOGENOMi | HOG000021111 |
KOi | K00052 |
OMAi | EYDLGAR |
OrthoDBi | 1551125at2 |
Family and domain databases
HAMAPi | MF_01035 LeuB_type2, 1 hit |
InterProi | View protein in InterPro IPR023698 3-isopropylmalate_DH_LeuB IPR019818 IsoCit/isopropylmalate_DH_CS IPR024084 IsoPropMal-DH-like_dom |
Pfami | View protein in Pfam PF00180 Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329 Iso_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00470 IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
D6ZGP3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSIDLALIP GDGIGHEVVP EAVKVLKKVL DGAGVDLKTT LYDLGAARYH
60 70 80 90 100
RDGVVLDEAT EQSLRGHDAI LLGAVGDPSV QSGVLERGLL LKLRFDFDHY
110 120 130 140 150
VNLRPSKYYP GVPTPLSNPG NIDFVVVREG TEGLYCGNGG VLRQGTPQEI
160 170 180 190 200
ATEVSVNTRH GVERVVRYAF AQAQSRRQHL TYVHKHNVLV NAGHLWRRCV
210 220 230 240 250
EEVGEEFPEV KVDYLHVDAA TIFLVTNPSR FDVIVTDNLF GDILTDEAGA
260 270 280 290 300
ITGGIGLAAS GNINPEKNFP SMFEPVHGSA PDIAGQGKAD PTATIASLVL
310 320 330 340 350
MLNHLDYPQL AAHIQRAIDA DMAHRAEASA AGTPLARTTS EIGDAIAAYL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001992 Genomic DNA Translation: ADI67801.1 |
RefSeqi | WP_013189413.1, NC_014246.1 |
Genome annotation databases
EnsemblBacteriai | ADI67801; ADI67801; HMPREF0573_11482 |
KEGGi | mcu:HMPREF0573_11482 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001992 Genomic DNA Translation: ADI67801.1 |
RefSeqi | WP_013189413.1, NC_014246.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 548479.HMPREF0573_11482 |
Genome annotation databases
EnsemblBacteriai | ADI67801; ADI67801; HMPREF0573_11482 |
KEGGi | mcu:HMPREF0573_11482 |
Phylogenomic databases
HOGENOMi | HOG000021111 |
KOi | K00052 |
OMAi | EYDLGAR |
OrthoDBi | 1551125at2 |
Enzyme and pathway databases
UniPathwayi | UPA00048;UER00072 |
BioCyci | GCF_000196535-HMP:HMPREF0573_RS07245-MONOMER |
Family and domain databases
HAMAPi | MF_01035 LeuB_type2, 1 hit |
InterProi | View protein in InterPro IPR023698 3-isopropylmalate_DH_LeuB IPR019818 IsoCit/isopropylmalate_DH_CS IPR024084 IsoPropMal-DH-like_dom |
Pfami | View protein in Pfam PF00180 Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329 Iso_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00470 IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | D6ZGP3_MOBCV | |
Accessioni | D6ZGP3Primary (citable) accession number: D6ZGP3 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | August 10, 2010 |
Last sequence update: | August 10, 2010 | |
Last modified: | April 10, 2019 | |
This is version 55 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |