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Protein

Bifunctional aspartokinase/homoserine dehydrogenase

Gene

ECDG_02798

Organism
Escherichia coli B185
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.UniRule annotation
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_02798), Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_04519), Aspartokinase (ECDG_02444)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-homoserine from L-aspartate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_02798), Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_04519), Aspartokinase (ECDG_02444)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_02798), Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_04519)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-threonine from L-aspartate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_02798), Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_04519), Aspartokinase (ECDG_02444)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_02798), Bifunctional aspartokinase/homoserine dehydrogenase (ECDG_04519)
  4. Homoserine kinase (thrB)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, OxidoreductaseUniRule annotation, Transferase
Biological processAmino-acid biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, NADPUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00034;UER00015

UPA00050;UER00063

UPA00050;UER00461

UPA00051;UER00462

UPA00051;UER00465

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenaseUniRule annotation
Including the following 2 domains:
AspartokinaseUniRule annotation (EC:2.7.2.4UniRule annotation)
Homoserine dehydrogenaseUniRule annotation (EC:1.1.1.3UniRule annotation)
Gene namesi
ORF Names:ECDG_02798Imported
OrganismiEscherichia coli B185Imported
Taxonomic identifieri550676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000004669 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD6IHW3
SMRiD6IHW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 394ACTInterPro annotationAdd BLAST75
Domaini401 – 478ACTInterPro annotationAdd BLAST78

Sequence similaritiesi

In the C-terminal section; belongs to the homoserine dehydrogenase family.UniRule annotation
In the N-terminal section; belongs to the aspartokinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.1160.10, 2 hits
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR005106 Asp/hSer_DH_NAD-bd
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR011147 Bifunc_aspartokin/hSer_DH
IPR027795 CASTOR_ACT_dom
IPR001342 HDH_cat
IPR019811 HDH_CS
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01842 ACT, 1 hit
PF13840 ACT_7, 1 hit
PF00742 Homoserine_dh, 1 hit
PF03447 NAD_binding_3, 1 hit
PIRSFiPIRSF000727 ThrA, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR00657 asp_kinases, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS00324 ASPARTOKINASE, 1 hit
PS01042 HOMOSER_DHGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

D6IHW3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM
60 70 80 90 100
IEKTISGQDA LPNISDAERI FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ
110 120 130 140 150
IKHVLHGISL LGQCPDSINA ALICRGEKMS IAIMAGVLEA RGHNVTVIDP
160 170 180 190 200
VEKLLAVGHY LESTVDIAES TRRIAASRIP ADHMVLMAGF TAGNEKGELV
210 220 230 240 250
VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV PDARLLKSMS
260 270 280 290 300
YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTGNPQA PGTLIGASRD
310 320 330 340 350
EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT
360 370 380 390 400
QSSSEYSISF CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS
410 420 430 440 450
VVGDGMRTLR GISAKFFAAL ARANINIVAI AQGSSERSIS VVVNNDDATT
460 470 480 490 500
GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL LEQLKRQQSW LKNKHIDLRV
510 520 530 540 550
CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL VKEYHLLNPV
560 570 580 590 600
IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR
610 620 630 640 650
RKFLYDTNVG AGLPVIENLQ NLLNAGDELM KFSGILSGSL SYIFGKLDEG
660 670 680 690 700
MSFSEATTLA REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE
710 720 730 740 750
IEPVLPAEFN AEGDVAAFMA NLSQLDDLFA ARVAKARDEG KVLRYVGNID
760 770 780 790 800
EDGVCRVKIA EVDGNDPLFK VKNGENALAF YSHYYQPLPL VLRGYGAGND
810 820
VTAAGVFADL LRTLSWKLGV
Length:820
Mass (Da):89,120
Last modified:July 13, 2010 - v1
Checksum:i0BF28E9EECAB10ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG749190 Genomic DNA Translation: EFF03594.2
RefSeqiWP_001264707.1, NZ_GG749190.1

Genome annotation databases

EnsemblBacteriaiEFF03594; EFF03594; ECDG_02798

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG749190 Genomic DNA Translation: EFF03594.2
RefSeqiWP_001264707.1, NZ_GG749190.1

3D structure databases

ProteinModelPortaliD6IHW3
SMRiD6IHW3
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEFF03594; EFF03594; ECDG_02798

Enzyme and pathway databases

UniPathwayi
UPA00034;UER00015

UPA00050;UER00063

UPA00050;UER00461

UPA00051;UER00462

UPA00051;UER00465

Family and domain databases

Gene3Di3.40.1160.10, 2 hits
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR005106 Asp/hSer_DH_NAD-bd
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR011147 Bifunc_aspartokin/hSer_DH
IPR027795 CASTOR_ACT_dom
IPR001342 HDH_cat
IPR019811 HDH_CS
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01842 ACT, 1 hit
PF13840 ACT_7, 1 hit
PF00742 Homoserine_dh, 1 hit
PF03447 NAD_binding_3, 1 hit
PIRSFiPIRSF000727 ThrA, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR00657 asp_kinases, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS00324 ASPARTOKINASE, 1 hit
PS01042 HOMOSER_DHGENASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiD6IHW3_ECOLX
AccessioniPrimary (citable) accession number: D6IHW3
Entry historyiIntegrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: March 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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