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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Methanocaldococcus infernus (strain DSM 11812 / JCM 15783 / ME)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.UniRule annotation

Catalytic activityi

DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation
DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei117Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationImported, Transferase
Biological processDNA damage, DNA repairUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:Metin_1269Imported
OrganismiMethanocaldococcus infernus (strain DSM 11812 / JCM 15783 / ME)Imported
Taxonomic identifieri573063 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000002061 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi573063.Metin_1269

Structurei

3D structure databases

ProteinModelPortaliD5VTL6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 144DNA_binding_1InterPro annotationAdd BLAST76

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02724 Archaea
COG0350 LUCA
HOGENOMiHOG000244137
KOiK00567
OMAiILKLYFA
OrthoDBiPOG093Z0E1E

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00772 OGT, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR023546 MGMT
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

D5VTL6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKINNYYIG LIFKDGYLVE NTIPLKCKLE GYSEDGEEIA KIILKLYLAE
60 70 80 90 100
LSDKEARKRI RFKLLTSDFT KRVLKEVCKI PFGETITYGE LAEKLKSSPR
110 120 130 140 150
AVGRALNKNP LPLIIPCHRV IAKDGVGGYK YGVEEKIKIL ERERNALLRA

NKI
Length:153
Mass (Da):17,485
Last modified:July 13, 2010 - v1
Checksum:i63E0AC0721236C61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002009 Genomic DNA Translation: ADG13919.1

Genome annotation databases

EnsemblBacteriaiADG13919; ADG13919; Metin_1269
KEGGimif:Metin_1269

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002009 Genomic DNA Translation: ADG13919.1

3D structure databases

ProteinModelPortaliD5VTL6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi573063.Metin_1269

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADG13919; ADG13919; Metin_1269
KEGGimif:Metin_1269

Phylogenomic databases

eggNOGiarCOG02724 Archaea
COG0350 LUCA
HOGENOMiHOG000244137
KOiK00567
OMAiILKLYFA
OrthoDBiPOG093Z0E1E

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00772 OGT, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR023546 MGMT
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiD5VTL6_METIM
AccessioniPrimary (citable) accession number: D5VTL6
Entry historyiIntegrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: October 25, 2017
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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