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Protein

Endo-1,4-beta-xylanase/feruloyl esterase

Gene

xyn10D-fae1A

Organism
Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in degradation of plant cell wall polysaccharides. Has endo-xylanase activity towards substrates such as oat spelt xylan (OSX), acetylated xylo-oligosaccharides and acetylated xylan, producing primarily xylobiose; cannot hydrolyze xylobiose to xylose. Also has feruloyl esterase activity, releasing ferulic acid from methylferulate, and from the more natural substrates wheat bran, corn fiber, and XOS(FA,Ac), a corn fiber-derived substrate enriched in O-acetyl and ferulic acid esters. Exhibits negligible acetyl esterase activity on sugar acetates. Acts synergistically with Xyl3A to increase the release of xylose from xylan. Does not possess endoglucanase or mannanase activities since it is not able to hydrolyze carboxymethyl cellulose and locust bean gum.2 Publications

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.2 Publications
Feruloyl-polysaccharide + H2O = ferulate + polysaccharide.2 Publications

pH dependencei

Optimum pH is 6.0 for xylanase activity.1 Publication

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.2 Publications
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Proton donor; for xylanase activityBy similarity1
Active sitei280Nucleophile; for xylanase activity1 Publication1
Active sitei629Nucleophile; for esterase activity1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Multifunctional enzyme
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

BioCyciPRUM264731:G1GHW-2816-MONOMER
UniPathwayi
UPA00114

Protein family/group databases

CAZyiGH10 Glycoside Hydrolase Family 10

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase/feruloyl esterase2 Publications
Including the following 2 domains:
Endo-1,4-beta-xylanase (EC:3.2.1.81 Publication)
Feruloyl esterase (EC:3.1.1.731 Publication)
Alternative name(s):
Ferulic acid esterase
Gene namesi
Name:xyn10D-fae1AImported
Ordered Locus Names:PRU_2728
ORF Names:ORF02827
OrganismiPrevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
Taxonomic identifieri264731 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPrevotellaceaePrevotella
Proteomesi
  • UP000000927 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi280E → S: Marked decrease in xylanase activity. Shows approximately 3-fold increase in feruloyl esterase activity. 1 Publication1
Mutagenesisi629S → A: Marked decrease in feruloyl esterase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000042240520 – 726Endo-1,4-beta-xylanase/feruloyl esteraseSequence analysisAdd BLAST707

Expressioni

Inductioni

By growth on ester-enriched corn oligosaccharides.1 Publication

Interactioni

Subunit structurei

Monomer or homodimer.1 Publication

Protein-protein interaction databases

STRINGi264731.PRU_2728

Structurei

3D structure databases

ProteinModelPortaliD5EY13
SMRiD5EY13
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 369GH10PROSITE-ProRule annotationAdd BLAST343

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni370 – 726Feruloyl esteraseAdd BLAST357

Domaini

The two catalytic domains appear to be functionally coupled.1 Publication

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 10 (cellulase F) family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DYC Bacteria
COG2382 LUCA
COG3693 LUCA
OrthoDBiPOG091H0Y2G

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR001000 GH10
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
PF00331 Glyco_hydro_10, 1 hit
PRINTSiPR00134 GLHYDRLASE10
SMARTiView protein in SMART
SM00633 Glyco_10, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF53474 SSF53474, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51760 GH10_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D5EY13-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKLLVALSL IAGSLTASAQ WGRPVDYAAG PGLKDAYKDY FTVGVAVNKF
60 70 80 90 100
NISDPAQTAI VKKQFNSVTA ENAWKPGEIH PKEGVWNFGL ADSIANFCRE
110 120 130 140 150
NGIKMRGHCL CWHSQFADWM FTDKKGKPVK KEVFYQRLRE HIHTVVNRYK
160 170 180 190 200
DVVYAWDVVN EAMADDGRPF EFVDGKMVPA SPYRQSRHFK LCGDEFIAKA
210 220 230 240 250
FEFAREADPT GVLMYNDYSC VDEGKRERIY NMVKKMKEAG VPIDGIGMQG
260 270 280 290 300
HYNIYFPDEE KLEKAINRFS EIVNTIHITE LDLRTNTESG GQLMFSRGEA
310 320 330 340 350
KPQPGYMQTL QEDQYARLFK IFRKHKDVIK NVTFWNLSDK DSWLGVNNHP
360 370 380 390 400
LPFDENFKAK RSLQIIRDFD AAMDNRKPKE DFVPNPMNQP GQEYPMVNSE
410 420 430 440 450
GYARFRVEAP DAKSVIVSLG LGGRGGTVLR KDKNGVWTGT TEGPMDPGFH
460 470 480 490 500
YYHLTIDGGV FNDPGTHNYF GSCRWESGIE IPAKDQDFYA YRKDINHGNI
510 520 530 540 550
QQVTFWSEST GKMQTANVYL PYGYGKVVKG KQERYPVLYL QHGWGENETS
560 570 580 590 600
WPVQGKAGLI MDNLIADGKI KPFIVVMAYG LTNDFKFGSI GKFTAEEFEK
610 620 630 640 650
VLIDELIPTI DKNFLTKADK WNRAMAGLSM GGMETKLITL RRPEMFGYWG
660 670 680 690 700
LLSGGTYMPE EIKDPKAVKY IFVGCGDKEN PEGINKSVEA LKAAGFKAEG
710 720
LVSEGTAHEF LTWRRCLEKM AQSLFK
Length:726
Mass (Da):82,289
Last modified:June 15, 2010 - v1
Checksum:i8C8641934CF95EA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ713437 Genomic DNA Translation: ACN78954.1
CP002006 Genomic DNA Translation: ADE83639.1
RefSeqiWP_013065621.1, NC_014033.1

Genome annotation databases

EnsemblBacteriaiADE83639; ADE83639; PRU_2728
GeneIDi31502259
KEGGipru:PRU_2728

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ713437 Genomic DNA Translation: ACN78954.1
CP002006 Genomic DNA Translation: ADE83639.1
RefSeqiWP_013065621.1, NC_014033.1

3D structure databases

ProteinModelPortaliD5EY13
SMRiD5EY13
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264731.PRU_2728

Protein family/group databases

CAZyiGH10 Glycoside Hydrolase Family 10

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE83639; ADE83639; PRU_2728
GeneIDi31502259
KEGGipru:PRU_2728

Phylogenomic databases

eggNOGiENOG4105DYC Bacteria
COG2382 LUCA
COG3693 LUCA
OrthoDBiPOG091H0Y2G

Enzyme and pathway databases

UniPathwayi
UPA00114

BioCyciPRUM264731:G1GHW-2816-MONOMER

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000801 Esterase_put
IPR001000 GH10
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00756 Esterase, 1 hit
PF00331 Glyco_hydro_10, 1 hit
PRINTSiPR00134 GLHYDRLASE10
SMARTiView protein in SMART
SM00633 Glyco_10, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF53474 SSF53474, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS51760 GH10_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiXYFA_PRER2
AccessioniPrimary (citable) accession number: D5EY13
Secondary accession number(s): C0LJN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: June 15, 2010
Last modified: February 28, 2018
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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