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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Streptococcus suis (strain GZ1)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Metal bindingi44PotassiumUniRule annotation1
Binding sitei57MethionineUniRule annotation1
Binding sitei100MethionineUniRule annotation1
Binding sitei251ATP; shared with neighboring subunitUniRule annotation1
Binding sitei251Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei274ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei278ATP; shared with neighboring subunitUniRule annotation1
Binding sitei282MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 177ATPUniRule annotation3
Nucleotide bindingi242 – 243ATPUniRule annotation2
Nucleotide bindingi257 – 258ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Biological processOne-carbon metabolismUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:SSGZ1_1453Imported
OrganismiStreptococcus suis (strain GZ1)Imported
Taxonomic identifieri423211 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002359 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Structurei

3D structure databases

SMRiD5AJ94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 102S-AdoMet_synt_NInterPro annotationAdd BLAST98
Domaini126 – 243S-AdoMet_synt_MInterPro annotationAdd BLAST118
Domaini245 – 384S-AdoMet_synt_CInterPro annotationAdd BLAST140

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 110Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

D5AJ94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERKLFTSE SVSEGHPDKI ADQISDAILD AILAEDPDAH VAAETAVYTG
60 70 80 90 100
SVHVFGEIST TAYVDINRVV RDTIAEIGYT KGEYGFSAES VGVHPSLVEQ
110 120 130 140 150
SPDIAQGVNE ALETRQDASQ DPLDLIGAGD QGLMFGFAVD ETPELMPLPI
160 170 180 190 200
SLSHKLVRRL AELRKSGEIA YLRPDAKSQV TVEYDEDNQP VRVDTVVIST
210 220 230 240 250
QHDPEVSQEQ IRQDVIERVI KEIIPAHYLD DQTNYFINPT GRFVIGGPQG
260 270 280 290 300
DSGLTGRKII VDTYGGYSRH GGGAFSGKDA TKVDRSASYA ARYIAKNIVA
310 320 330 340 350
AGLAKKAEVQ LAYAIGVAHP VSVRIDTFGT STVAESKLEA AVRHIFDLRP
360 370 380 390
AGIIQMLDLK RPIYKQTAAY GHMGRTDIDL PWEKLDKVEA LKTAVL
Length:396
Mass (Da):43,088
Last modified:June 15, 2010 - v1
Checksum:iA903824A3218914A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000837 Genomic DNA Translation: ADE31909.1
RefSeqiWP_012027546.1, NC_017617.1

Genome annotation databases

EnsemblBacteriaiADE31909; ADE31909; SSGZ1_1453
KEGGissw:SSGZ1_1453
PATRICifig|423211.3.peg.1432

Similar proteinsi

Entry informationi

Entry nameiD5AJ94_STRGZ
AccessioniPrimary (citable) accession number: D5AJ94
Entry historyiIntegrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: March 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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