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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma alligatoris A21JP2
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei547Aminoacyl-adenylateUniRule annotation1
Binding sitei591ATPUniRule annotation1
Metal bindingi858ZincUniRule annotation1
Metal bindingi861ZincUniRule annotation1
Metal bindingi875ZincUniRule annotation1
Metal bindingi878ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
ORF Names:MALL_0292Imported
OrganismiMycoplasma alligatoris A21JP2Imported
Taxonomic identifieri747682 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000004757 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi747682.MALL_0292

Structurei

3D structure databases

ProteinModelPortaliD4XWX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 627tRNA-synt_1InterPro annotationAdd BLAST599
Domaini672 – 819Anticodon_1InterPro annotationAdd BLAST148

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionUniRule annotationAdd BLAST11
Motifi588 – 592"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

D4XWX2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYKKTLIMP NTKFEMKANL TQKEALFRKE WEDNHVYKLV LEKYKNSPRF
60 70 80 90 100
VLHDGPPYAN GDLHVGHALN KILKDIIVRY KTLRGFYSPF VPGWDTHGLP
110 120 130 140 150
IEHRMLTEAK INKDELTPII LRKKAAKYAY KQIENQKKQF KTLQMLSDFE
160 170 180 190 200
NIYITMDKKF EANQLKLFKK MTLDGLVYKG LKPVYWSPSS QSALAEAEVE
210 220 230 240 250
YQDVTSPSIY VAFEVTESNQ SAVKKGDHLV IWTTTPWTLI ANAGVALGEE
260 270 280 290 300
FSYSRVEYEN KFYFIATDLV EKFAEEAQWE NYKIVSKHKA SEIVGVKYLT
310 320 330 340 350
PILKKLAPVI IGHHVTLEAG TGLVHIAPLF GEDDFLIGKK HKLDMIMHIA
360 370 380 390 400
DNGHVDYECK YKTFFYEKAN KFIIQDLTEE KTLLKANKIK HSYPHDWRTH
410 420 430 440 450
QPIMFRGTPQ WFVSIDKIKK DIIDQILKVK TYPVWAQKRL VQMIENRNDW
460 470 480 490 500
TISRQRTWGV PITIFYDKDK NPVIKEDIFD YVIALVEQFG CDVWWEKSTD
510 520 530 540 550
ELLPEKYRNL GFTREMDIMD VWFDSGSTSI AIDNIEGVEA PFDVYLEGID
560 570 580 590 600
QYRGWFNSSV INSVAYRGKA PYKTLISHGF VLDGKGEKMS KSKGNIISPL
610 620 630 640 650
DIVKKNGADI LRLWAANSEY SNDVNISEKI IEQNSEIYRK VRNTLRFLLG
660 670 680 690 700
NLNDYKYDKN QKREDIHLFI KEELNQLKIQ VLKAYDDFKF INVIKMVNKY
710 720 730 740 750
IVSLSGFYLS VTKDILYVDK KDSERRKQTL ANFYEIVEFL IFALGPIMPT
760 770 780 790 800
TIEETYKHFN NENKKISFML EDYDFTKLAV DQAYLEKFNE FFELRDRVNI
810 820 830 840 850
LVETGIKNGL IKRSNEANVF VNEPSEFLKS LELKQLLMVG NVEFSQEEKL
860 870 880 890
EVFESEKCQR CWNHFALKEF KDELCLNCFE IIKDLVPNEQ
Length:890
Mass (Da):104,134
Last modified:June 15, 2010 - v1
Checksum:i87741DEFB726440B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ADNC01000027 Genomic DNA Translation: EFF41204.1
RefSeqiWP_005683823.1, NZ_ADNC01000027.1

Genome annotation databases

EnsemblBacteriaiEFF41204; EFF41204; MALL_0292

Similar proteinsi

Entry informationi

Entry nameiD4XWX2_9MOLU
AccessioniPrimary (citable) accession number: D4XWX2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: March 28, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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