UniProtKB - D4GYI2 (GLPA1_HALVD)
Protein
Anaerobic glycerol-3-phosphate dehydrogenase subunit A1
Gene
gpdA1
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Functioni
Conversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Required for growth on glycerol and for glycerol metabolism.1 Publication
Miscellaneous
H.volcanii contains 2 glpABC operons, one located on the main chromosome and the other on megaplasmid pHV4.1 Publication
Catalytic activityi
- EC:1.1.5.31 Publication
Cofactori
Protein has several cofactor binding sites:Activity regulationi
Up-regulated by glycerol and no inhibition by glucose.1 Publication
: glycerol degradation via glycerol kinase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).Proteins known to be involved in this subpathway in this organism are:
- Glycerol-3-phosphate dehydrogenase (C498_09986), Anaerobic glycerol-3-phosphate dehydrogenase subunit A2 (gpdA2), Anaerobic glycerol-3-phosphate dehydrogenase subunit A1 (gpdA1), Glycerol-3-phosphate dehydrogenase (C498_03045)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 6 – 34 | FADSequence analysisAdd BLAST | 29 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: InterPro
- FMN binding Source: InterPro
- sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC
GO - Biological processi
- glycerol-3-phosphate metabolic process Source: InterPro
- glycerol catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
UniPathwayi | UPA00618;UER00673 |
Names & Taxonomyi
Protein namesi | Recommended name: Anaerobic glycerol-3-phosphate dehydrogenase subunit A1 (EC:1.1.5.3)Short name: G-3-P dehydrogenase A1 Short name: G3PDH A1 |
Gene namesi | Name:gpdA1 Ordered Locus Names:HVO_1538 |
Organismi | Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) |
Taxonomic identifieri | 309800 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Stenosarchaea group › Halobacteria › Haloferacales › Haloferacaceae › Haloferax › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- glycerol-3-phosphate dehydrogenase complex Source: InterPro
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Unable to grow on glycerol. Can be complemented in trans by glpA2 under the control from a strong promoter.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000428859 | 1 – 586 | Anaerobic glycerol-3-phosphate dehydrogenase subunit A1Add BLAST | 586 |
Expressioni
Inductioni
Up-regulated by glycerol 3-phosphate.1 Publication
Interactioni
Subunit structurei
Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.
By similarityProtein-protein interaction databases
STRINGi | 309800.C498_03045 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 537 – 585 | Asp-richAdd BLAST | 49 |
Sequence similaritiesi
Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | arCOG00753, Archaea arCOG05746, Archaea |
HOGENOMi | CLU_015740_0_1_2 |
OMAi | GVMTIMN |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR000447, G3P_DH_FAD-dep IPR017752, G3P_DH_GlpA_su |
PANTHERi | PTHR11985, PTHR11985, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF04324, Fer2_BFD, 1 hit |
PRINTSi | PR01001, FADG3PDH |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03377, glycerol3P_GlpA, 1 hit |
PROSITEi | View protein in PROSITE PS00977, FAD_G3PDH_1, 1 hit PS00978, FAD_G3PDH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
D4GYI2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKSPSVLVI GGGSTGTGIA RDLAMRGLDV TLVEKGNLTH GTTGRMHGLL
60 70 80 90 100
HSGGRYAVSD QPSAKECIEE NRVLRRIAGH CVEMTGGLFV QRPEDSDEYF
110 120 130 140 150
EKKLEGCREC GIPAEVLSAE EAREIEPYLA KDIKRAIKVP DGAVDPFRLC
160 170 180 190 200
VANAASAVEH GARIETHSEV TDVLVEGGEV VGVEVTHQTG TGPYVHGEPG
210 220 230 240 250
EVEEIRADYV VNATGAWAGQ IGDFAGVNVE VRPSKGVMTI MNTRQVDTVV
260 270 280 290 300
NRCRPKGDAD IIVPHETTCI LGTTDEEVED PEDYPEEGWE VDLMIETLSE
310 320 330 340 350
LVPMLADART IRSFWGVRPL YEPPGTGTED PTDITREFFL LDHADRDDLP
360 370 380 390 400
GMTSIVGGKL TTYRMMAEQI SDHVCEKLGV DAECRTADEP LPGSEDFTVL
410 420 430 440 450
RDYMDDFGLR SPIGRRSAQR LGSRADEVLN SVDPNPVVCE CEAVTRAEIQ
460 470 480 490 500
DALDTAGTDL NSVRIQTRAS MGNCQGAICC HRMANELAPE YDEKTVRASL
510 520 530 540 550
DDLYQERWKG ERHAMWGTQL SQTALKHMLH AATMNRDEDP AAADADIDFA
560 570 580
AFDDGVASGG AVADGGRERA ADRADDDALG GADGDN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001956 Genomic DNA Translation: ADE05130.1 |
RefSeqi | WP_013035654.1, NC_013967.1 |
Genome annotation databases
EnsemblBacteriai | ADE05130; ADE05130; HVO_1538 |
GeneIDi | 8925946 |
KEGGi | hvo:HVO_1538 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001956 Genomic DNA Translation: ADE05130.1 |
RefSeqi | WP_013035654.1, NC_013967.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 309800.C498_03045 |
Genome annotation databases
EnsemblBacteriai | ADE05130; ADE05130; HVO_1538 |
GeneIDi | 8925946 |
KEGGi | hvo:HVO_1538 |
Phylogenomic databases
eggNOGi | arCOG00753, Archaea arCOG05746, Archaea |
HOGENOMi | CLU_015740_0_1_2 |
OMAi | GVMTIMN |
Enzyme and pathway databases
UniPathwayi | UPA00618;UER00673 |
Family and domain databases
Gene3Di | 1.10.10.1100, 1 hit 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR007419, BFD-like_2Fe2S-bd_dom IPR041854, BFD-like_2Fe2S-bd_dom_sf IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR000447, G3P_DH_FAD-dep IPR017752, G3P_DH_GlpA_su |
PANTHERi | PTHR11985, PTHR11985, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF04324, Fer2_BFD, 1 hit |
PRINTSi | PR01001, FADG3PDH |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR03377, glycerol3P_GlpA, 1 hit |
PROSITEi | View protein in PROSITE PS00977, FAD_G3PDH_1, 1 hit PS00978, FAD_G3PDH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLPA1_HALVD | |
Accessioni | D4GYI2Primary (citable) accession number: D4GYI2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 14, 2014 |
Last sequence update: | May 18, 2010 | |
Last modified: | December 2, 2020 | |
This is version 57 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families