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UniProtKB - D4GSE6 (KDGK1_HALVD)
Protein
2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase
Gene
kdgK1
Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Functioni
Involved in the degradation of glucose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG). Also catalyzes efficiently the phosphorylation of 2-keto-3-deoxygalactonate (KDGal) to 2-keto-3-deoxy-6-phosphogalactonate (KDPGal).
1 PublicationCatalytic activityi
- 2-dehydro-3-deoxy-D-gluconate + ATP = 2-dehydro-3-deoxy-6-phospho-D-gluconate + ADP + H+1 PublicationEC:2.7.1.1781 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-dehydro-3-deoxy-D-galactonate + ATP = 2-dehydro-3-deoxy-6-phospho-D-galactonate + ADP + H+1 PublicationEC:2.7.1.1781 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=0.54 mM for KDG1 Publication
- KM=0.73 mM for KDGal1 Publication
- KM=0.36 mM for ATP1 Publication
- Vmax=78 µmol/min/mg enzyme with KDG as substrate1 Publication
- Vmax=33 µmol/min/mg enzyme with KDGal as substrate1 Publication
: 2-dehydro-3-deoxy-D-gluconate degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.Curated This subpathway is part of the pathway 2-dehydro-3-deoxy-D-gluconate degradation, which is itself part of Carbohydrate acid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | SubstrateBy similarity | 1 | |
Binding sitei | 171 | SubstrateBy similarity | 1 | |
Active sitei | 262 | Proton acceptorBy similarity | 1 | |
Binding sitei | 262 | SubstrateBy similarity | 1 | |
Binding sitei | 298 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 169 – 171 | ATPBy similarity | 3 | |
Nucleotide bindingi | 230 – 235 | ATPBy similarity | 6 | |
Nucleotide bindingi | 259 – 262 | ATPBy similarity | 4 |
GO - Molecular functioni
- 2-dehydro-3-deoxygalactonokinase activity Source: RHEA
- 2-dehydro-3-deoxygluconokinase activity Source: RHEA
- ATP binding Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.1.178, 2561 2.7.1.45, 2561 |
UniPathwayi | UPA00856;UER00828 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinaseCurated (EC:2.7.1.1781 Publication)Short name: 2-dehydro-3-deoxyglucono/galactono-kinaseCurated Alternative name(s): 2-keto-3-deoxygluconate/2-keto-3-deoxygalactonate kinaseCurated KDG kinase1 Publication KDGK-11 Publication |
Gene namesi | Name:kdgK11 Publication Ordered Locus Names:HVO_0549Imported |
Organismi | Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) |
Taxonomic identifieri | 309800 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Stenosarchaea group › Halobacteria › Haloferacales › Haloferacaceae › Haloferax › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion of the gene abolishes growth on glucose, but does not affect growth on galactose. Double deletion mutant kdgK1/kdgK2 loses the ability to grow on galactose.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000449269 | 1 – 320 | 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinaseAdd BLAST | 320 |
Expressioni
Inductioni
Constitutively expressed.1 Publication
Interactioni
Subunit structurei
Homohexamer.
1 PublicationProtein-protein interaction databases
STRINGi | 309800.C498_15984 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 38 | Substrate bindingBy similarity | 5 | |
Regioni | 107 – 109 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the carbohydrate kinase PfkB family.Curated
Phylogenomic databases
eggNOGi | arCOG00014, Archaea |
HOGENOMi | CLU_027634_0_1_2 |
OMAi | MAMFYAN |
OrthoDBi | 36944at2157 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
i Sequence
Sequence statusi: Complete.
D4GSE6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTALVTFGET MLRLSPPRGE RLETARELEV QAGGAESNVA VAAARLGRDA
60 70 80 90 100
AWFSKLPDSP LGRRIVSELR SHSVDTDGVV WTDDADARQG VYYLEHGASP
110 120 130 140 150
RPTNVVYDRA DAAVTTLETG EFDLDAVRDA EVCFTSGITP ALSETLSETT
160 170 180 190 200
ADVLDEAQNA GTTTAFDLNY RTKLWSPDEA AEVYRDLLDS VDLLFAAERD
210 220 230 240 250
AATVLGRDGD AESVARGLAD DYDIETVVVT RGEEGSLAVS DGAVSEQGVY
260 270 280 290 300
ETETYDAIGT GDAFVGGFLA KHLDGGSVTE SLEWASATAS FKRTVEGDIA
310 320
VVTPEDVERV VAEEGDGISR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001956 Genomic DNA Translation: ADE04815.1 |
RefSeqi | WP_004044382.1, NZ_AOHU01000099.1 |
Genome annotation databases
EnsemblBacteriai | ADE04815; ADE04815; HVO_0549 |
GeneIDi | 8924883 |
KEGGi | hvo:HVO_0549 |
PATRICi | fig|309800.29.peg.3092 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001956 Genomic DNA Translation: ADE04815.1 |
RefSeqi | WP_004044382.1, NZ_AOHU01000099.1 |
3D structure databases
AlphaFoldDBi | D4GSE6 |
SMRi | D4GSE6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 309800.C498_15984 |
Genome annotation databases
EnsemblBacteriai | ADE04815; ADE04815; HVO_0549 |
GeneIDi | 8924883 |
KEGGi | hvo:HVO_0549 |
PATRICi | fig|309800.29.peg.3092 |
Phylogenomic databases
eggNOGi | arCOG00014, Archaea |
HOGENOMi | CLU_027634_0_1_2 |
OMAi | MAMFYAN |
OrthoDBi | 36944at2157 |
Enzyme and pathway databases
UniPathwayi | UPA00856;UER00828 |
BRENDAi | 2.7.1.178, 2561 2.7.1.45, 2561 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KDGK1_HALVD | |
Accessioni | D4GSE6Primary (citable) accession number: D4GSE6 Secondary accession number(s): L9UMS7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 22, 2020 |
Last sequence update: | May 18, 2010 | |
Last modified: | May 25, 2022 | |
This is version 63 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families