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UniProtKB - D4AV26 (MNS1B_ARTBC)
Protein
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase ARB_00035
Gene
ARB_00035
Organism
Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
By similarityCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity, Mg2+By similarityNote: Ca2+. Can also use Mg2+, but with lower efficiency.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 374 | Proton donorBy similarity | 1 | |
Metal bindingi | 500 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable mannosyl-oligosaccharide alpha-1,2-mannosidase ARB_00035Curated (EC:3.2.1.113By similarity)Alternative name(s): Class I alpha-mannosidaseBy similarity Man(9)-alpha-mannosidaseBy similarity |
Gene namesi | ORF Names:ARB_00035 |
Organismi | Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) (Trichophyton mentagrophytes) |
Taxonomic identifieri | 663331 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Arthrodermataceae › Trichophyton › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- membrane Source: InterPro
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_5003053524 | 19 – 509 | Probable mannosyl-oligosaccharide alpha-1,2-mannosidase ARB_00035Add BLAST | 491 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 170 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 331 ↔ 360 | By similarity | ||
Glycosylationi | 399 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 663331.D4AV26 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
HOGENOMi | CLU_003818_0_2_1 |
OMAi | YSYLIHA |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
D4AV26-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSLSLLGVS SLALTSLALP TMNQNQHRFV ALADLADEDK PQAIKDAFNH
60 70 80 90 100
AWKGYMTHAY PMDELRPVSN EGSNPLNGWG ATPVDALSTA IIMGLPDVVN
110 120 130 140 150
QILDHISKID FSKTDDLCSL FETTIRYLGG LISGYDLLKN SGAMNADPEK
160 170 180 190 200
VDVLLKKAVE LADVLKFSFN STTGIPSNTL NIPKQTTDGA TSNGLATVGT
210 220 230 240 250
LVLEWTRLSD LTNKEEYGKL AQKAEEYLLN PQPKSNEVFP GLVGGSINIQ
260 270 280 290 300
TGKFERASAS WEGGDDSFYE YLIKMYVYDR SAFGKYKDRW VLAAQSTIEH
310 320 330 340 350
LKSSPLLHPD TTFVGAWANG ALVKSSQHLA CFDGGNFILG GRELNRPEFT
360 370 380 390 400
RFGLKLVDGC HKTYANTVTK IGPESFGWDE KNVPSDQASF FTKSGFYINS
410 420 430 440 450
SGYVLRPEVI ESFYYAYRVT GDKKYQRWIW DAFVAISETT KTDSGFSSIS
460 470 480 490 500
DVNAAKGGSK TDSQPSFFFA ETLKYTYLAF SDEADWQISR SGKDKFVFNT
EAHPIRVRN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | ABSU01000012 Genomic DNA Translation: EFE32948.1 |
RefSeqi | XP_003013588.1, XM_003013542.1 |
Genome annotation databases
EnsemblFungii | EFE32948; EFE32948; ARB_00035 |
GeneIDi | 9519867 |
KEGGi | abe:ARB_00035 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | ABSU01000012 Genomic DNA Translation: EFE32948.1 |
RefSeqi | XP_003013588.1, XM_003013542.1 |
3D structure databases
SMRi | D4AV26 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 663331.D4AV26 |
Genome annotation databases
EnsemblFungii | EFE32948; EFE32948; ARB_00035 |
GeneIDi | 9519867 |
KEGGi | abe:ARB_00035 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
HOGENOMi | CLU_003818_0_2_1 |
OMAi | YSYLIHA |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MNS1B_ARTBC | |
Accessioni | D4AV26Primary (citable) accession number: D4AV26 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 9, 2015 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 23, 2022 | |
This is version 51 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families