UniProtKB - D3ZW55 (ITPA_RAT)
Protein
Inosine triphosphate pyrophosphatase
Gene
Itpa
Organism
Rattus norvegicus (Rat)
Status
Functioni
Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.UniRule annotation
Catalytic activityi
- a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H+UniRule annotationEC:3.6.1.9UniRule annotation
- a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H+UniRule annotationEC:3.6.1.9UniRule annotation
Cofactori
Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 56 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 72 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 172 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- dITP diphosphatase activity Source: Ensembl
- identical protein binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- NADH pyrophosphatase activity Source: UniProtKB-EC
- nucleoside-triphosphate diphosphatase activity Source: GO_Central
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- chromosome organization Source: Ensembl
- deoxyribonucleoside triphosphate catabolic process Source: UniProtKB-UniRule
- ITP catabolic process Source: Ensembl
- nucleoside triphosphate catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-74259 Purine catabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Inosine triphosphate pyrophosphataseUniRule annotation (EC:3.6.1.9UniRule annotation)Short name: ITPaseUniRule annotation Short name: Inosine triphosphataseUniRule annotation Alternative name(s): Non-canonical purine NTP pyrophosphataseUniRule annotation Non-standard purine NTP pyrophosphataseUniRule annotation Nucleoside-triphosphate diphosphataseUniRule annotation Nucleoside-triphosphate pyrophosphataseUniRule annotation Short name: NTPaseUniRule annotation |
Gene namesi | Name:Itpa |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1589751 Itpa |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedUniRule annotation | |||
ChainiPRO_0000413101 | 2 – 198 | Inosine triphosphate pyrophosphataseAdd BLAST | 197 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineUniRule annotationBy similarity | 1 | |
Modified residuei | 146 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | D3ZW55 |
PaxDbi | D3ZW55 |
PeptideAtlasi | D3ZW55 |
PRIDEi | D3ZW55 |
PTM databases
SwissPalmi | D3ZW55 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000021233 Expressed in 10 organ(s), highest expression level in colon |
Genevisiblei | D3ZW55 RN |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000028838 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 19 | Substrate bindingUniRule annotation | 6 | |
Regioni | 72 – 73 | Substrate bindingUniRule annotation | 2 | |
Regioni | 149 – 152 | Substrate bindingUniRule annotation | 4 | |
Regioni | 177 – 178 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the HAM1 NTPase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3222 Eukaryota COG0127 LUCA |
GeneTreei | ENSGT00390000015399 |
InParanoidi | D3ZW55 |
KOi | K01519 |
OMAi | YDPIFQP |
OrthoDBi | 1298391at2759 |
PhylomeDBi | D3ZW55 |
TreeFami | TF105614 |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_03148 HAM1_NTPase, 1 hit |
InterProi | View protein in InterPro IPR002637 Ham1p-like IPR027502 ITPase IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
D3ZW55-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAASLVGKKI VFVTGNAKKL EEVIQILGDK FPCTLVAQKI DLPEYQGEPD
60 70 80 90 100
EISIQKCQEA ARQVQGPVLV EDTCLCFNAL GGLPGPYIKW FLQKLKPEGL
110 120 130 140 150
YQLLAGFEDK SAYALCTFAL STGDPSQPVL LFRGKTPGQI VMPRGSRDFG
160 170 180 190
WDPCFQPDGY EQTYAEMPKA EKNTISHRFR ALFKLQEYFG VTDGAGDH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473949 Genomic DNA Translation: EDL80207.1 |
RefSeqi | NP_001101244.1, NM_001107774.1 |
Genome annotation databases
Ensembli | ENSRNOT00000028838; ENSRNOP00000028838; ENSRNOG00000021233 |
GeneIDi | 311422 |
KEGGi | rno:311422 |
UCSCi | RGD:1589751 rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473949 Genomic DNA Translation: EDL80207.1 |
RefSeqi | NP_001101244.1, NM_001107774.1 |
3D structure databases
SMRi | D3ZW55 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000028838 |
PTM databases
SwissPalmi | D3ZW55 |
Proteomic databases
jPOSTi | D3ZW55 |
PaxDbi | D3ZW55 |
PeptideAtlasi | D3ZW55 |
PRIDEi | D3ZW55 |
Genome annotation databases
Ensembli | ENSRNOT00000028838; ENSRNOP00000028838; ENSRNOG00000021233 |
GeneIDi | 311422 |
KEGGi | rno:311422 |
UCSCi | RGD:1589751 rat |
Organism-specific databases
CTDi | 3704 |
RGDi | 1589751 Itpa |
Phylogenomic databases
eggNOGi | KOG3222 Eukaryota COG0127 LUCA |
GeneTreei | ENSGT00390000015399 |
InParanoidi | D3ZW55 |
KOi | K01519 |
OMAi | YDPIFQP |
OrthoDBi | 1298391at2759 |
PhylomeDBi | D3ZW55 |
TreeFami | TF105614 |
Enzyme and pathway databases
Reactomei | R-RNO-74259 Purine catabolism |
Miscellaneous databases
PROi | PR:D3ZW55 |
Gene expression databases
Bgeei | ENSRNOG00000021233 Expressed in 10 organ(s), highest expression level in colon |
Genevisiblei | D3ZW55 RN |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_03148 HAM1_NTPase, 1 hit |
InterProi | View protein in InterPro IPR002637 Ham1p-like IPR027502 ITPase IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ITPA_RAT | |
Accessioni | D3ZW55Primary (citable) accession number: D3ZW55 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | April 20, 2010 | |
Last modified: | May 8, 2019 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families