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Protein

AP-2 complex subunit alpha

Gene

Ap2a1

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei53Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei57Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei58Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei61Phosphatidylinositol lipid headgroupUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosisUniRule annotation, Protein transportUniRule annotationSAAS annotation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-177504 Retrograde neurotrophin signalling
R-RNO-2132295 MHC class II antigen presentation
R-RNO-416993 Trafficking of GluR2-containing AMPA receptors
R-RNO-437239 Recycling pathway of L1
R-RNO-5099900 WNT5A-dependent internalization of FZD4
R-RNO-5140745 WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis
R-RNO-8866427 VLDLR internalisation and degradation
R-RNO-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alphaUniRule annotation
Gene namesi
Name:Ap2a1Imported
Synonyms:Ap2a1_predictedImported
ORF Names:rCG_54084Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1306254 Ap2a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Coated pitUniRule annotation, Membrane

PTM / Processingi

Proteomic databases

PRIDEiD3ZUY8

Expressioni

Gene expression databases

BgeeiENSRNOG00000026243 Expressed in 9 organ(s), highest expression level in brain

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

IntActiD3ZUY8, 2 interactors
STRINGi10116.ENSRNOP00000020304

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini745 – 858Alpha_adaptinC2InterPro annotationAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 43Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG1077 Eukaryota
ENOG410XNQE LUCA
GeneTreeiENSGT00550000074757
KOiK11824
OMAiHNELFNC
OrthoDBiEOG091G01JZ
TreeFamiTF300308

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.310.10, 1 hit
InterProiView protein in InterPro
IPR017104 AP2_complex_asu
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
IPR003164 Clathrin_a-adaptin_app_sub_C
IPR008152 Clathrin_a/b/g-adaptin_app_Ig
IPR013041 Clathrin_app_Ig-like_sf
IPR009028 Coatomer/calthrin_app_sub_C
IPR012295 TBP_dom_sf
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF02296 Alpha_adaptin_C, 1 hit
PF02883 Alpha_adaptinC2, 1 hit
PIRSFiPIRSF037091 AP2_complex_alpha, 1 hit
SMARTiView protein in SMART
SM00809 Alpha_adaptinC2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF49348 SSF49348, 1 hit
SSF55711 SSF55711, 1 hit

Sequencei

Sequence statusi: Complete.

D3ZUY8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD
160 170 180 190 200
IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH
210 220 230 240 250
MGVVTAAVSL ITCLCKKNPD DFKTCVSLAV SRLSRIVSSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV
310 320 330 340 350
QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL
360 370 380 390 400
ESMCTLASSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS
410 420 430 440 450
NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI
460 470 480 490 500
RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG
510 520 530 540 550
GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN
560 570 580 590 600
LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE
610 620 630 640 650
EMPPFPERES SILAKLKRKK GPGAASALDD SRRDTSSNDI NGGVEPTPSN
660 670 680 690 700
VSTPSPSADL LGLRAAPPPA APPAPVGGNL LVDVFSDGPT AQPSLGPTPE
710 720 730 740 750
EAFLSELEPP APESPMTLLA DPAPAADPGP EDIGPPIPEA DELLNKFVCK
760 770 780 790 800
NSGVLFENQL LQIGVKSEFR QNLGRMYLFY GNKTSVQFQS FLPTVVHPGD
810 820 830 840 850
LQTHLAVQTK RVAAQVDGGA QVQQVLNIEC LRDFLTPPLL SVRFRYGGTA
860 870 880 890 900
QSLTLKLPVT INKFFQPTEM AAQDFFQRWK QLSLPLQEAQ KIFKANHPMD
910 920 930 940 950
AEVTKAKLLG FGSALLDNVD PNPENFVGAG IIQTKALQVG CLLRLEPNAQ
960 970
AQMYRLTLRT SKEPVSRHLC ELLAQQF
Length:977
Mass (Da):107,675
Last modified:April 20, 2010 - v1
Checksum:i5704048BEC825FDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC094894 Genomic DNA No translation available.
CH473979 Genomic DNA Translation: EDM07441.1
RefSeqiNP_001100981.1, NM_001107511.2
UniGeneiRn.162416

Genome annotation databases

EnsembliENSRNOT00000020304; ENSRNOP00000020304; ENSRNOG00000026243
GeneIDi308578
KEGGirno:308578
UCSCiRGD:1306254 rat

Similar proteinsi

Entry informationi

Entry nameiD3ZUY8_RAT
AccessioniPrimary (citable) accession number: D3ZUY8
Entry historyiIntegrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: September 12, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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