UniProtKB - D3ZTE0 (FA12_RAT)
Coagulation factor XII
F12
Functioni
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (By similarity).
By similarityCatalytic activityi
- Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa. EC:3.4.21.38
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 393 | Charge relay systemBy similarity | 1 | |
Active sitei | 442 | Charge relay systemBy similarity | 1 | |
Active sitei | 543 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- peptidase activity Source: RGD
- serine-type endopeptidase activity Source: RGD
GO - Biological processi
- blood coagulation Source: RGD
- Factor XII activation Source: RGD
- fibrinolysis Source: UniProtKB-KW
- plasma kallikrein-kinin cascade Source: RGD
- positive regulation of blood coagulation Source: RGD
- positive regulation of fibrinolysis Source: RGD
- positive regulation of plasminogen activation Source: RGD
- protein autoprocessing Source: RGD
- protein processing Source: RGD
- proteolysis Source: GO_Central
- response to misfolded protein Source: RGD
- zymogen activation Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Blood coagulation, Fibrinolysis, Hemostasis |
Enzyme and pathway databases
BRENDAi | 3.4.21.38, 5301 |
Reactomei | R-RNO-140837, Intrinsic Pathway of Fibrin Clot Formation |
Protein family/group databases
MEROPSi | S01.211 |
Names & Taxonomyi
Protein namesi | Recommended name: Coagulation factor XII (EC:3.4.21.38)Alternative name(s): Hageman factor Short name: HAF Cleaved into the following 2 chains: |
Gene namesi | Name:F12 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359175, F12 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Endoplasmic reticulum
- rough endoplasmic reticulum Source: GO_Central
Extracellular region or secreted
- extracellular space Source: RGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | By similarityAdd BLAST | 19 | |
ChainiPRO_0000394557 | 20 – 353 | Coagulation factor XIIa heavy chainAdd BLAST | 334 | |
ChainiPRO_0000394558 | 354 – 595 | Coagulation factor XIIa light chainAdd BLAST | 242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 72 | By similarity | ||
Disulfide bondi | 60 ↔ 87 | By similarity | ||
Disulfide bondi | 97 ↔ 109 | By similarity | ||
Disulfide bondi | 103 ↔ 118 | By similarity | ||
Glycosylationi | 108 | O-linked (Fuc) threonineBy similarity | 1 | |
Disulfide bondi | 120 ↔ 129 | By similarity | ||
Disulfide bondi | 134 ↔ 162 | By similarity | ||
Disulfide bondi | 160 ↔ 169 | By similarity | ||
Disulfide bondi | 177 ↔ 188 | By similarity | ||
Disulfide bondi | 182 ↔ 197 | By similarity | ||
Disulfide bondi | 199 ↔ 208 | By similarity | ||
Disulfide bondi | 216 ↔ 294 | By similarity | ||
Disulfide bondi | 237 ↔ 276 | By similarity | ||
Glycosylationi | 248 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 265 ↔ 289 | By similarity | ||
Glycosylationi | 298 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Glycosylationi | 307 | O-linked (GalNAc...) serineBy similarity | 1 | |
Glycosylationi | 326 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Disulfide bondi | 340 ↔ 466 | By similarity | ||
Disulfide bondi | 378 ↔ 394 | By similarity | ||
Disulfide bondi | 386 ↔ 455 | By similarity | ||
Glycosylationi | 414 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 417 ↔ 420 | By similarity | ||
Disulfide bondi | 480 ↔ 549 | By similarity | ||
Disulfide bondi | 512 ↔ 528 | By similarity | ||
Disulfide bondi | 539 ↔ 570 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | D3ZTE0 |
PeptideAtlasi | D3ZTE0 |
PRIDEi | D3ZTE0 |
PTM databases
GlyGeni | D3ZTE0, 6 sites |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000015139, Expressed in liver and 16 other tissues |
ExpressionAtlasi | D3ZTE0, baseline and differential |
Genevisiblei | D3ZTE0, RN |
Interactioni
Subunit structurei
Interacts with HRG; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding, inhibits factor XII autoactivation and contact-initiated coagulation.
By similarityProtein-protein interaction databases
IntActi | D3ZTE0, 1 interactor |
STRINGi | 10116.ENSRNOP00000061983 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 89 | Fibronectin type-IIPROSITE-ProRule annotationAdd BLAST | 49 | |
Domaini | 93 – 130 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 132 – 172 | Fibronectin type-IPROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 173 – 209 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 215 – 294 | KringlePROSITE-ProRule annotationAdd BLAST | 80 | |
Domaini | 354 – 594 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 241 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 302 – 332 | DisorderedSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Kringle, Repeat, SignalPhylogenomic databases
eggNOGi | KOG1217, Eukaryota KOG3627, Eukaryota |
GeneTreei | ENSGT00940000161657 |
InParanoidi | D3ZTE0 |
OrthoDBi | 1314811at2759 |
PhylomeDBi | D3ZTE0 |
TreeFami | TF329901 |
Family and domain databases
CDDi | cd00061, FN1, 1 hit cd00062, FN2, 1 hit cd00108, KR, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.10.10.10, 1 hit 2.40.10.10, 2 hits 2.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR014394, Coagulation_fac_XII/HGFA IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000083, Fibronectin_type1 IPR000562, FN_type2_dom IPR036943, FN_type2_sf IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00008, EGF, 2 hits PF00039, fn1, 1 hit PF00040, fn2, 1 hit PF00051, Kringle, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001146, Factor_XII_HGFA, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 2 hits SM00058, FN1, 1 hit SM00059, FN2, 1 hit SM00130, KR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01186, EGF_2, 1 hit PS50026, EGF_3, 2 hits PS01253, FN1_1, 1 hit PS51091, FN1_2, 1 hit PS00023, FN2_1, 1 hit PS51092, FN2_2, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MTALLFLGSL LMSLDLTLSA PPWKSKEFKD GAGDPSVVLT VDGKLCHFPF
60 70 80 90 100
QYHRRLYHKC IHKGQPGSRP WCATTPNFDE DQQWGYCLEP KKVKDHCSKH
110 120 130 140 150
SPCHKGGTCV NTPNGPHCLC PEHLTGKHCQ REKCFESQLL KFFHENEIWF
160 170 180 190 200
RTGPGGVARC QCKGPQAVCK LLTSQVCRVN PCLNGGTCLL VEDHRLCHCP
210 220 230 240 250
AGYAGPFCDL DLKATCYEDR GLSYRGQAKT TLSGAPCQRW ASEATYRNMT
260 270 280 290 300
ETQALSWGLG HHAFCRNPDN DTRPWCYVWS GDRLSWDYCD LEQCQMPTLT
310 320 330 340 350
SPVSPESHDM LKPRPPILQS SPRDSTRNQN VVSRTSTVVC GQRFRKRLSS
360 370 380 390 400
LRRVVGGLVA LPGSHPYIAA LYWGDSFCAG SLIDPCWVLT AAHCLQKRPA
410 420 430 440 450
PEELTVVLGQ DRHNQSCERC QTLAVHSYRL HEGFSSKTYQ HDLALLRLRG
460 470 480 490 500
RKNSCAILSP HVQPVCLPSS AAPPSETVLC EVAGWGHQFE GAEEYATFLQ
510 520 530 540 550
EAQVPFISLD RCSSSNVHGD AILPGMLCAG FLEGGADACQ GDSGGPLVCD
560 570 580 590
EGVTERQLTL RGVISWGSGC GDRNKPGVYT DVANYLDWIQ EHTAF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UI19 | A0A0H2UI19_RAT | Coagulation factor XII | F12 | 603 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 320 – 324 | SSPRD → MPQFPSLSDALDN in AAH88187 (PubMed:15489334).Curated | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474032 Genomic DNA Translation: EDL93987.1 Sequence problems. BC088187 mRNA Translation: AAH88187.1 |
RefSeqi | NP_001014028.1, NM_001014006.1 |
Genome annotation databases
Ensembli | ENSRNOT00000081920; ENSRNOP00000074115; ENSRNOG00000015139 |
GeneIDi | 306761 |
KEGGi | rno:306761 |
UCSCi | RGD:1359175, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474032 Genomic DNA Translation: EDL93987.1 Sequence problems. BC088187 mRNA Translation: AAH88187.1 |
RefSeqi | NP_001014028.1, NM_001014006.1 |
3D structure databases
SMRi | D3ZTE0 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | D3ZTE0, 1 interactor |
STRINGi | 10116.ENSRNOP00000061983 |
Protein family/group databases
MEROPSi | S01.211 |
PTM databases
GlyGeni | D3ZTE0, 6 sites |
Proteomic databases
PaxDbi | D3ZTE0 |
PeptideAtlasi | D3ZTE0 |
PRIDEi | D3ZTE0 |
Genome annotation databases
Ensembli | ENSRNOT00000081920; ENSRNOP00000074115; ENSRNOG00000015139 |
GeneIDi | 306761 |
KEGGi | rno:306761 |
UCSCi | RGD:1359175, rat |
Organism-specific databases
CTDi | 2161 |
RGDi | 1359175, F12 |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota KOG3627, Eukaryota |
GeneTreei | ENSGT00940000161657 |
InParanoidi | D3ZTE0 |
OrthoDBi | 1314811at2759 |
PhylomeDBi | D3ZTE0 |
TreeFami | TF329901 |
Enzyme and pathway databases
BRENDAi | 3.4.21.38, 5301 |
Reactomei | R-RNO-140837, Intrinsic Pathway of Fibrin Clot Formation |
Miscellaneous databases
PROi | PR:D3ZTE0 |
Gene expression databases
Bgeei | ENSRNOG00000015139, Expressed in liver and 16 other tissues |
ExpressionAtlasi | D3ZTE0, baseline and differential |
Genevisiblei | D3ZTE0, RN |
Family and domain databases
CDDi | cd00061, FN1, 1 hit cd00062, FN2, 1 hit cd00108, KR, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.10.10.10, 1 hit 2.40.10.10, 2 hits 2.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR014394, Coagulation_fac_XII/HGFA IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000083, Fibronectin_type1 IPR000562, FN_type2_dom IPR036943, FN_type2_sf IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00008, EGF, 2 hits PF00039, fn1, 1 hit PF00040, fn2, 1 hit PF00051, Kringle, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001146, Factor_XII_HGFA, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 2 hits SM00058, FN1, 1 hit SM00059, FN2, 1 hit SM00130, KR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 2 hits |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01186, EGF_2, 1 hit PS50026, EGF_3, 2 hits PS01253, FN1_1, 1 hit PS51091, FN1_2, 1 hit PS00023, FN2_1, 1 hit PS51092, FN2_2, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FA12_RAT | |
Accessioni | D3ZTE0Primary (citable) accession number: D3ZTE0 Secondary accession number(s): Q5M879 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 15, 2010 |
Last sequence update: | April 20, 2010 | |
Last modified: | February 23, 2022 | |
This is version 76 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families