Mafa

Functionⁱ

Transcription factor that activates insulin gene expression (PubMed:15665000). Acts synergistically with NEUROD1/BETA2 and PDX1 (By similarity). Binds the insulin enhancer C1/RIPE3b element (PubMed:15665000). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (By similarity).By similarity1 Publication

GO - Molecular functionⁱ

RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

insulin secretion Source: Ensembl
nitric oxide mediated signal transduction Source: Ensembl
response to glucose Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription factor MafA Alternative name(s): Pancreatic beta-cell-specific transcriptional activator
Gene namesⁱ	Name:Mafa
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 7

Organism-specific databases

Rat genome database More...RGDⁱ	1562627 Mafa

RGDⁱ

1562627 Mafa

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000445278	1 – 361	Transcription factor MafAAdd BLAST		361

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	14	PhosphoserineBy similarity	1
Cross-linkⁱ	32	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	49	PhosphoserineBy similarity	1
Modified residueⁱ	53	PhosphothreonineBy similarity	1
Modified residueⁱ	57	PhosphothreonineBy similarity	1
Modified residueⁱ	61	PhosphoserineBy similarity	1
Modified residueⁱ	65	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Ubiquitinated, leading to its degradation by the proteasome.By similarity

Phosphorylated at tyrosines.By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	D3ZNT6

PaxDbⁱ

D3ZNT6

Expressionⁱ

Inductionⁱ

Up-regulated by glucose in Langerhans islets (at protein level).1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000007668 Expressed in 8 organ(s), highest expression level in skeletal muscle tissue

Bgeeⁱ

ENSRNOG00000007668 Expressed in 8 organ(s), highest expression level in skeletal muscle tissue

Interactionⁱ

Subunit structureⁱ

Forms homodimers. Monomers and dimers are able to bind DNA, but the off-rate is faster for monomers (By similarity). Interacts with NEUROD1 and PDX1 (By similarity). May interact with MAFB, FOS, JUN and PCAF (By similarity).By similarity

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	262 – 325	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	262 – 287	Basic motifPROSITE-ProRule annotationAdd BLAST		26
Regionⁱ	290 – 311	Leucine-zipperPROSITE-ProRule annotationAdd BLAST		22

Sequence similaritiesⁱ

Belongs to the bZIP family.SAAS annotation

Phylogenomic databases

eggNOGⁱ	ENOG410KDH9 Eukaryota ENOG4112BJ1 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074549
InParanoidⁱ	D3ZNT6
KEGG Orthology (KO) More...KOⁱ	K07595
OMAⁱ	QRHILES
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0H46
PhylomeDBⁱ	D3ZNT6
TreeFamⁱ	TF325689

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR004826 bZIP_Maf IPR013592 Maf_TF_N IPR028562 MafA IPR008917 TF_DNA-bd_sf IPR024874 Transciption_factor_Maf_fam
PANTHERⁱ	PTHR10129 PTHR10129, 1 hit PTHR10129:SF30 PTHR10129:SF30, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03131 bZIP_Maf, 1 hit PF08383 Maf_N, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
SUPFAMⁱ	SSF47454 SSF47454, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

D3ZNT6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAELAMGAE LPSSPLAIEY VNDFDLMKFE VKKEPPEAER FCHRLPPGSL 
        60         70         80         90        100
SSTPLSTPCS SVPSSPSFCA PSPGTGSSAG GGGSAAQAGG APGPPSGGPG 
       110        120        130        140        150
TVGGASGKAV LEDLYWMSGY QHHLNPEALN LTPEDAVEAL IGSGHHSAHH 
       160        170        180        190        200
GAHHPAAAAA YEAFRGQSFA GGGGGGADDM GAGHHHGAHH TAHHHHSAHH 
       210        220        230        240        250
HHHHHHHHGG SGHHGGGAGH GGGGAGHHVR LEERFSDDQL VSMSVRELNR 
       260        270        280        290        300
QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ 
       310        320        330        340        350
VEQLKLEVGR LAKERDLYKE KYEKLAGRGG PGGAGGAGFP REPSPAQAGP 
       360 
GAAKGAPDFF L

Length:361

Mass (Da):37,780

Last modified:April 20, 2010 - v1

Checksum:ⁱ0055A8D0DBE65620

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC133673 Genomic DNA No translation available.
NCBI Reference Sequences More...RefSeqⁱ	XP_006241965.1, XM_006241903.2

Genome annotation databases

Ensemblⁱ	ENSRNOT00000010084; ENSRNOP00000010084; ENSRNOG00000007668
GeneIDⁱ	366949
KEGGⁱ	rno:366949
UCSC genome browser More...UCSCⁱ	RGD:1562627 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
D3ZNT6	Transcription factor MafA	)	HUMAN		353	UniRef90_Q8NHW3
Transcription factor MafA		MOUSE		359
transcription factor MafA		MESAU		361
Uncharacterized protein		ICTTR		360
MAFA isoform 2		PONAB		359
+46

Protein	Similar proteins		Species	Score	Length	Source
D3ZNT6	Transcription factor MafA	)	HUMAN		353	UniRef50_Q8NHW3
Transcription factor MafA		MOUSE		359
transcription factor MafA		MESAU		361
Uncharacterized protein		ICTTR		360
MAFA isoform 2		PONAB		359
+47

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC133673 Genomic DNA No translation available.
RefSeqⁱ	XP_006241965.1, XM_006241903.2

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PaxDbⁱ	D3ZNT6

PaxDbⁱ

D3ZNT6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000010084; ENSRNOP00000010084; ENSRNOG00000007668
GeneIDⁱ	366949
KEGGⁱ	rno:366949
UCSCⁱ	RGD:1562627 rat

Organism-specific databases

CTDⁱ	389692
RGDⁱ	1562627 Mafa

Phylogenomic databases

eggNOGⁱ	ENOG410KDH9 Eukaryota ENOG4112BJ1 LUCA
GeneTreeⁱ	ENSGT00550000074549
InParanoidⁱ	D3ZNT6
KOⁱ	K07595
OMAⁱ	QRHILES
OrthoDBⁱ	EOG091G0H46
PhylomeDBⁱ	D3ZNT6
TreeFamⁱ	TF325689

Gene expression databases

Bgeeⁱ	ENSRNOG00000007668 Expressed in 8 organ(s), highest expression level in skeletal muscle tissue

Bgeeⁱ

ENSRNOG00000007668 Expressed in 8 organ(s), highest expression level in skeletal muscle tissue

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR004826 bZIP_Maf IPR013592 Maf_TF_N IPR028562 MafA IPR008917 TF_DNA-bd_sf IPR024874 Transciption_factor_Maf_fam
PANTHERⁱ	PTHR10129 PTHR10129, 1 hit PTHR10129:SF30 PTHR10129:SF30, 1 hit
Pfamⁱ	View protein in Pfam PF03131 bZIP_Maf, 1 hit PF08383 Maf_N, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
SUPFAMⁱ	SSF47454 SSF47454, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAFA_RAT
Accessionⁱ	D3ZNT6Primary (citable) accession number: D3ZNT6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 10, 2018
	Last sequence update:	April 20, 2010
	Last modified:	October 10, 2018
	This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - D3ZNT6 (MAFA_RAT)

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Transcription factor MafA

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ