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UniProtKB - D3ZNT6 (MAFA_RAT)
Protein
Transcription factor MafA
Gene
Mafa
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcription factor that activates insulin gene expression (PubMed:15665000).
Acts synergistically with NEUROD1/BETA2 and PDX1 (By similarity).
Binds the insulin enhancer C1/RIPE3b element (PubMed:15665000).
Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (By similarity).
By similarity1 PublicationGO - Molecular functioni
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- insulin secretion Source: RGD
- nitric oxide mediated signal transduction Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to glucose Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor MafAAlternative name(s): Pancreatic beta-cell-specific transcriptional activator |
Gene namesi | Name:Mafa |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1562627, Mafa |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000445278 | 1 – 361 | Transcription factor MafAAdd BLAST | 361 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineBy similarity | 1 | |
Cross-linki | 32 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 49 | PhosphoserineBy similarity | 1 | |
Modified residuei | 53 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 57 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 61 | PhosphoserineBy similarity | 1 | |
Modified residuei | 65 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated, leading to its degradation by the proteasome.By similarity
Phosphorylated at tyrosines.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | D3ZNT6 |
Expressioni
Inductioni
Up-regulated by glucose in Langerhans islets (at protein level).1 Publication
Gene expression databases
Bgeei | ENSRNOG00000007668, Expressed in quadriceps femoris and 18 other tissues |
Interactioni
Subunit structurei
Forms homodimers. Monomers and dimers are able to bind DNA, but the off-rate is faster for monomers (By similarity).
Interacts with NEUROD1 and PDX1 (By similarity). May interact with MAFB, FOS, JUN and PCAF (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000010084 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 262 – 325 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 40 – 105 | DisorderedSequence analysisAdd BLAST | 66 | |
Regioni | 175 – 228 | DisorderedSequence analysisAdd BLAST | 54 | |
Regioni | 262 – 287 | Basic motifPROSITE-ProRule annotationAdd BLAST | 26 | |
Regioni | 290 – 311 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 22 | |
Regioni | 324 – 361 | DisorderedSequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 49 – 82 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 187 – 213 | Basic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Belongs to the bZIP family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000162747 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | D3ZNT6 |
OMAi | GAHHTAH |
OrthoDBi | 1395389at2759 |
PhylomeDBi | D3ZNT6 |
TreeFami | TF325689 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR046347, bZIP_sf IPR013592, Maf_TF_N IPR028562, MafA IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF30, PTHR10129:SF30, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
i Sequence
Sequence statusi: Complete.
D3ZNT6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAELAMGAE LPSSPLAIEY VNDFDLMKFE VKKEPPEAER FCHRLPPGSL
60 70 80 90 100
SSTPLSTPCS SVPSSPSFCA PSPGTGSSAG GGGSAAQAGG APGPPSGGPG
110 120 130 140 150
TVGGASGKAV LEDLYWMSGY QHHLNPEALN LTPEDAVEAL IGSGHHSAHH
160 170 180 190 200
GAHHPAAAAA YEAFRGQSFA GGGGGGADDM GAGHHHGAHH TAHHHHSAHH
210 220 230 240 250
HHHHHHHHGG SGHHGGGAGH GGGGAGHHVR LEERFSDDQL VSMSVRELNR
260 270 280 290 300
QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ
310 320 330 340 350
VEQLKLEVGR LAKERDLYKE KYEKLAGRGG PGGAGGAGFP REPSPAQAGP
360
GAAKGAPDFF L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC133673 Genomic DNA No translation available. |
RefSeqi | XP_006241965.1, XM_006241903.2 |
Genome annotation databases
Ensembli | ENSRNOT00000010084; ENSRNOP00000010084; ENSRNOG00000007668 |
GeneIDi | 366949 |
KEGGi | rno:366949 |
UCSCi | RGD:1562627, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC133673 Genomic DNA No translation available. |
RefSeqi | XP_006241965.1, XM_006241903.2 |
3D structure databases
AlphaFoldDBi | D3ZNT6 |
SMRi | D3ZNT6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000010084 |
Proteomic databases
PaxDbi | D3ZNT6 |
Genome annotation databases
Ensembli | ENSRNOT00000010084; ENSRNOP00000010084; ENSRNOG00000007668 |
GeneIDi | 366949 |
KEGGi | rno:366949 |
UCSCi | RGD:1562627, rat |
Organism-specific databases
CTDi | 389692 |
RGDi | 1562627, Mafa |
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000162747 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | D3ZNT6 |
OMAi | GAHHTAH |
OrthoDBi | 1395389at2759 |
PhylomeDBi | D3ZNT6 |
TreeFami | TF325689 |
Miscellaneous databases
PROi | PR:D3ZNT6 |
Gene expression databases
Bgeei | ENSRNOG00000007668, Expressed in quadriceps femoris and 18 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR046347, bZIP_sf IPR013592, Maf_TF_N IPR028562, MafA IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF30, PTHR10129:SF30, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAFA_RAT | |
Accessioni | D3ZNT6Primary (citable) accession number: D3ZNT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2018 |
Last sequence update: | April 20, 2010 | |
Last modified: | May 25, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families