UniProtKB - D3ZMK9 (PRAG1_RAT)
Inactive tyrosine-protein kinase PRAG1
Prag1
Functioni
Catalytically inactive protein kinase that acts as a scaffold protein (PubMed:29503074).
Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (PubMed:16481321).
Promotes Src family kinase (SFK) signallig by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (PubMed:27116701, PubMed:21873224).
Acts as a critical coactivator of Notch signaling (By similarity).
By similarity4 PublicationsCaution
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- protein kinase activity Source: GO_Central
GO - Biological processi
- cell migration Source: RGD
- negative regulation of neuron projection development Source: UniProtKB
- positive regulation of Rho protein signal transduction Source: UniProtKB
- protein phosphorylation Source: GO_Central
- regulation of cell motility Source: UniProtKB
- regulation of cell shape Source: UniProtKB
- regulation of Notch signaling pathway Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Inactive tyrosine-protein kinase PRAG1CuratedAlternative name(s): PEAK1-related kinase-activating pseudokinase 1 Pragma of Rnd21 Publication |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1311793, Prag1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Nucleus
- Nucleus By similarity
Other locations
- focal adhesion 1 Publication
Note: Colocalized with NOTCH1 in the nucleus.By similarity
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- focal adhesion Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 391 | Y → A: Does not affect cytoplasmic localization. 1 Publication | 1 | |
Mutagenesisi | 391 | Y → F: Decreases the interaction with CSK. 2 Publications | 1 | |
Mutagenesisi | 939 | L → E: Does not affect homodimerization. 1 Publication | 1 | |
Mutagenesisi | 978 | D → N: No ATP-binding activity. 1 Publication | 1 | |
Mutagenesisi | 981 | Y → F: No ATP-binding activity. 1 Publication | 1 | |
Mutagenesisi | 997 | K → A: Does not affect PRAG1-CSK complex formation. Does not affect PRAG1-induced protein tyrosine phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 1021 | Q → E: No ATP-binding activity. 1 Publication | 1 | |
Mutagenesisi | 1329 | A → E: Strong reduction of homodimerization; Does not affect PRAG1-CSK complex formation. Reduces CSK activation. 1 Publication | 1 | |
Mutagenesisi | 1349 | Y → E: Does not affect homodimerization. 1 Publication | 1 | |
Mutagenesisi | 1362 | L → E: Does not affect homodimerization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000444634 | 1 – 1368 | Inactive tyrosine-protein kinase PRAG1Add BLAST | 1368 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 238 | Phosphotyrosine; by CSK1 Publication | 1 | |
Modified residuei | 343 | Phosphotyrosine; by CSK1 Publication | 1 | |
Modified residuei | 391 | Phosphotyrosine; by CSK3 Publications | 1 | |
Modified residuei | 667 | PhosphoserineBy similarity | 1 | |
Modified residuei | 716 | PhosphoserineBy similarity | 1 | |
Modified residuei | 753 | PhosphoserineBy similarity | 1 | |
Modified residuei | 797 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | D3ZMK9 |
PTM databases
iPTMneti | D3ZMK9 |
PhosphoSitePlusi | D3ZMK9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000011407, Expressed in brain and 21 other tissues |
Interactioni
Subunit structurei
Homodimer (PubMed:29503074). Dimerization leads to the catalytic activation of CSK (PubMed:29503074).
Interacts (via C-terminus) with RND2 (PubMed:16481321).
Interacts with CSK (via SH2 domain) in a Tyr-391 phosphorylation-dependent manner; this interaction potentiates kinase activity of CSK (PubMed:29503074, PubMed:21873224, PubMed:27116701).
Interacts with NOTCH1 intracellular domain (N1ICD) (By similarity).
Forms a complex with N1ICD and MAML1, in a MAML1-dependent manner (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
ELMi | D3ZMK9 |
IntActi | D3ZMK9, 2 interactors |
STRINGi | 10116.ENSRNOP00000015166 |
Structurei
Secondary structure
3D structure databases
SMRi | D3ZMK9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 940 – 1291 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 352 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 200 – 236 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 297 – 330 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 392 – 443 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 499 – 605 | DisorderedSequence analysisAdd BLAST | 107 | |
Regioni | 636 – 792 | DisorderedSequence analysisAdd BLAST | 157 | |
Regioni | 799 – 818 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 873 – 901 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 906 – 949 | Required for homodimerization1 PublicationAdd BLAST | 44 | |
Regioni | 1134 – 1166 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 1293 – 1368 | Required for homodimerization1 PublicationAdd BLAST | 76 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 311 – 330 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 392 – 406 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 411 – 443 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 542 – 575 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 652 – 666 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 701 – 754 | Polar residuesSequence analysisAdd BLAST | 54 | |
Compositional biasi | 877 – 901 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 1134 – 1156 | Polar residuesSequence analysisAdd BLAST | 23 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QVUZ, Eukaryota |
GeneTreei | ENSGT00940000157066 |
HOGENOMi | CLU_005467_0_0_1 |
InParanoidi | D3ZMK9 |
OMAi | FKGNGHW |
OrthoDBi | 49921at2759 |
PhylomeDBi | D3ZMK9 |
TreeFami | TF331193 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008266, Tyr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSACSDFVEH IWKPGSCKNC FCLRSDHQLT AGHPKARASS LPAGARLPAR
60 70 80 90 100
PEICRLEDEG VNGLAYSKPT IAVKPTMMTS ETADLWTEAS LSAEVPKVNW
110 120 130 140 150
RRTPGKLLLQ KQEDGPIVYL GSFRGMQKAA GPLACTDSNS RCPPAYTMVG
160 170 180 190 200
LHNLEARVDR NTALQPVNFQ EEKAGREELP SAQESFRQKL AAFTGMTSSC
210 220 230 240 250
LKGPRPCTSP QPLRESLPSE DDSDQRCSPS GDSEGGEYCS ILDCRPESRD
260 270 280 290 300
AVHNTEGSGR RRGDCSPICW EQGTCTRPTE EEKQALNFPR ECCGQGSTAN
310 320 330 340 350
PPHLGPKKPS LNSEAASSSD GLSCGSSRSG ANSPFAPHLE NDYCSLVKEP
360 370 380 390 400
TSVKQQDSGC HLVNSGKYVG QAVDLQPPAL PREAVQPEPI YAESAKRKKA
410 420 430 440 450
APVPQRPEPK KEQVSSGQVW TGDTWSQKTP SGWSQEREGP NAAPQVATTI
460 470 480 490 500
TVIAAHPEED HRTIYLSSPD SAVGVQWPRG SLNQDLHGSG EEPLVVQGLS
510 520 530 540 550
SRESHPHNMT ENSSKEKPAI PPKLSKSSPG GSPVSPAAPP LTDHSDGNTS
560 570 580 590 600
GSSVGSQPSS RVPTNLTSSC QTNGVAAGDP AKCPPQANSS VLDQRRPRYQ
610 620 630 640 650
TGAWSRQCRI EEEEEVGQEL LSQSWGRELE NGIADHSNSS TWHRLHPIDG
660 670 680 690 700
ASGQNGKTNS GMSKSASFAF EFPKDRGRLE SFSPPPPPPK SRHLLKMNKS
710 720 730 740 750
SSDLEKVSQS SAESLSPSFR GAHVSFTTGS TDSLASDSRT CSDGGPSCEA
760 770 780 790 800
THSPTISGKK LFAPVPFPSG STEDVSPSGP AQPPPLPQKK IVSRAASSPD
810 820 830 840 850
GFFWTQGSPK PRTASPKLNL SHSETNVCAH DEPPLSYSLN SGNHPHHVFS
860 870 880 890 900
SSEPLEKAFK GSVPWAPALG PANSKGGCGS PNLQGRAATS TSSSQLSVSS
910 920 930 940 950
QASTGSSQLQ LHSLLSSISS KEGTYAKLGG LYTQSLARLV TKCEDLFMGG
960 970 980 990 1000
LKTELRFDEN SWSLFKLICN KPCCDSGDAI YYGATCSKDP DSIYAVKICK
1010 1020 1030 1040 1050
TPEPKSASYC SPSVPVHFNI QQDCGHFVAS VPSSMLAFPD TSSKDPAPAA
1060 1070 1080 1090 1100
PSHTPAQEQD CVVVITREVP HQTASDFVRD SVASHRAEPE VYERRVCFLL
1110 1120 1130 1140 1150
LQLCNGLEHL KEHGIIHRDL CLENLLLVHC NPQSSPGPSA NPSVPTTTSR
1160 1170 1180 1190 1200
CPSAAPAATT ACQGGPGEKH LPRLIISNFL KAKQKPGGTT NLQQKKSQAR
1210 1220 1230 1240 1250
LAPEIVSASQ YRKFDEFQTG ILIYELLHQP NPFEVRAQLR ERDYRREDLP
1260 1270 1280 1290 1300
PLPTLSLYSP GLQQLAHLLL EADPIKRIRI GEAKRVLQCL LWGPRRELVE
1310 1320 1330 1340 1350
QPCPSEEVLC NTLHNWIDMK RALMMMKFAE KAVERRRGVE LEDWLCCQYL
1360
ASAEPGALLQ SLKLLQLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR07026025 Genomic DNA No translation available. AABR07026026 Genomic DNA No translation available. CH473970 Genomic DNA Translation: EDM09203.1 |
RefSeqi | NP_001100785.1, NM_001107315.1 XP_017455620.1, XM_017600131.1 XP_017455621.1, XM_017600132.1 |
Genome annotation databases
Ensembli | ENSRNOT00000015166; ENSRNOP00000015166; ENSRNOG00000011407 |
GeneIDi | 306506 |
KEGGi | rno:306506 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR07026025 Genomic DNA No translation available. AABR07026026 Genomic DNA No translation available. CH473970 Genomic DNA Translation: EDM09203.1 |
RefSeqi | NP_001100785.1, NM_001107315.1 XP_017455620.1, XM_017600131.1 XP_017455621.1, XM_017600132.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EWX | X-ray | 2.77 | A/B | 906-1368 | [»] | |
SMRi | D3ZMK9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ELMi | D3ZMK9 |
IntActi | D3ZMK9, 2 interactors |
STRINGi | 10116.ENSRNOP00000015166 |
PTM databases
iPTMneti | D3ZMK9 |
PhosphoSitePlusi | D3ZMK9 |
Proteomic databases
PaxDbi | D3ZMK9 |
Genome annotation databases
Ensembli | ENSRNOT00000015166; ENSRNOP00000015166; ENSRNOG00000011407 |
GeneIDi | 306506 |
KEGGi | rno:306506 |
Organism-specific databases
CTDi | 157285 |
RGDi | 1311793, Prag1 |
Phylogenomic databases
eggNOGi | ENOG502QVUZ, Eukaryota |
GeneTreei | ENSGT00940000157066 |
HOGENOMi | CLU_005467_0_0_1 |
InParanoidi | D3ZMK9 |
OMAi | FKGNGHW |
OrthoDBi | 49921at2759 |
PhylomeDBi | D3ZMK9 |
TreeFami | TF331193 |
Miscellaneous databases
PROi | PR:D3ZMK9 |
Gene expression databases
Bgeei | ENSRNOG00000011407, Expressed in brain and 21 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008266, Tyr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRAG1_RAT | |
Accessioni | D3ZMK9Primary (citable) accession number: D3ZMK9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 18, 2018 |
Last sequence update: | April 20, 2010 | |
Last modified: | February 23, 2022 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families