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Entry version 73 (12 Aug 2020)
Sequence version 1 (20 Apr 2010)
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Protein

Microtubule-actin cross-linking factor 1

Gene

Macf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (By similarity). Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane (By similarity). Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics (By similarity). Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration (By similarity). May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4 (By similarity). Plays a key role in wound healing and epidermal cell migration (By similarity). Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells (By similarity). As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding motifs also exist.<p><a href='/help/ca_bind' target='_top'>More...</a></p>Calcium bindingi5096 – 51071PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi5132 – 51432PROSITE-ProRule annotationAdd BLAST12

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActin-binding
Biological processWnt signaling pathway
LigandCalcium, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Microtubule-actin cross-linking factor 1
Alternative name(s):
Actin cross-linking family 7
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Macf1
Synonyms:Acf7, Aclp7, Macf
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Rat genome database

More...
RGDi
1306057, Macf1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004097091 – 5430Microtubule-actin cross-linking factor 1Add BLAST5430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei4PhosphoserineCombined sources1
Modified residuei35PhosphoserineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Modified residuei280PhosphoserineCombined sources1
Modified residuei1122PhosphoserineBy similarity1
Modified residuei1367PhosphoserineBy similarity1
Modified residuei1376PhosphoserineBy similarity1
Modified residuei1860PhosphoserineCombined sources1
Modified residuei2429PhosphoserineCombined sources1
Modified residuei2454PhosphoserineCombined sources1
Modified residuei2769PhosphoserineBy similarity1
Modified residuei2895PhosphoserineBy similarity1
Modified residuei3368PhosphothreonineBy similarity1
Modified residuei4074PhosphoserineCombined sources1
Modified residuei4252N6-acetyllysineBy similarity1
Modified residuei5009PhosphoserineBy similarity1
Modified residuei5296PhosphothreonineCombined sources1
Modified residuei5321PhosphoserineBy similarity1
Modified residuei5334PhosphoserineBy similarity1
Modified residuei5372PhosphoserineBy similarity1
Modified residuei5375PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated on serine residues in the C-terminal tail by GSK3B. Phosphorylation inhibits microtubule-binding and this plays a critical role in bulge stem cell migration and skin wound repair. Wnt-signaling can repress phosphorylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
D3ZHV2

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
D3ZHV2

PeptideAtlas

More...
PeptideAtlasi
D3ZHV2

PRoteomics IDEntifications database

More...
PRIDEi
D3ZHV2

PTM databases

CarbonylDB database of protein carbonylation sites

More...
CarbonylDBi
D3ZHV2

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
D3ZHV2

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with MAPRE1, CLASP1, CLASP2 and GOLGA4 (By similarity).

Interacts with AXIN1 and LRP6 (PubMed:16815997).

Found in a complex composed of MACF1, APC; AXIN1, CTNNB1 and GSK3B (PubMed:16815997).

Interacts with CAMSAP3 (By similarity).

By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

CORUM comprehensive resource of mammalian protein complexes

More...
CORUMi
D3ZHV2

STRING: functional protein association networks

More...
STRINGi
10116.ENSRNOP00000041065

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
D3ZHV2

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini78 – 181Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST104
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati148 – 171LRR 1Add BLAST24
Domaini194 – 298Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST105
Repeati240 – 264LRR 2Add BLAST25
Repeati377 – 399LRR 3Add BLAST23
Repeati441 – 464LRR 4Add BLAST24
Domaini868 – 925SH3PROSITE-ProRule annotationAdd BLAST58
Repeati1050 – 1073LRR 5Add BLAST24
Repeati1128 – 1154LRR 6Add BLAST27
Repeati1187 – 1210LRR 7Add BLAST24
Repeati1257 – 1282LRR 8Add BLAST26
Repeati1579 – 1602LRR 9Add BLAST24
Repeati1629 – 1653LRR 10Add BLAST25
Repeati1816 – 1891Spectrin 1Add BLAST76
Repeati1869 – 1891LRR 11Add BLAST23
Repeati1933 – 2041Spectrin 2Add BLAST109
Repeati2058 – 2083LRR 12Add BLAST26
Repeati2194 – 2220LRR 13Add BLAST27
Repeati2399 – 2507Spectrin 3Add BLAST109
Repeati2444 – 2467LRR 14Add BLAST24
Repeati2534 – 2557LRR 15Add BLAST24
Repeati2702 – 2725LRR 16Add BLAST24
Repeati2733 – 2837Spectrin 4Add BLAST105
Repeati2842 – 2945Spectrin 5Add BLAST104
Repeati2984 – 3009LRR 17Add BLAST26
Repeati3105 – 3127LRR 18Add BLAST23
Repeati3169 – 3274Spectrin 6Add BLAST106
Repeati3214 – 3237LRR 19Add BLAST24
Repeati3281 – 3383Spectrin 7Add BLAST103
Repeati3388 – 3491Spectrin 8Add BLAST104
Repeati3714 – 3818Spectrin 9Add BLAST105
Repeati3737 – 3761LRR 20Add BLAST25
Repeati3825 – 3927Spectrin 10Add BLAST103
Repeati3846 – 3870LRR 21Add BLAST25
Repeati4047 – 4152Spectrin 11Add BLAST106
Repeati4157 – 4261Spectrin 12Add BLAST105
Repeati4267 – 4370Spectrin 13Add BLAST104
Repeati4375 – 4481Spectrin 14Add BLAST107
Repeati4486 – 4589Spectrin 15Add BLAST104
Repeati4538 – 4561LRR 22Add BLAST24
Repeati4594 – 4700Spectrin 16Add BLAST107
Repeati4707 – 4808Spectrin 17Add BLAST102
Repeati4812 – 4916Spectrin 18Add BLAST105
Domaini5083 – 5118EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini5119 – 5154EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini5159 – 5231GARPROSITE-ProRule annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 295Actin-bindingAdd BLAST295
Regioni5159 – 5430C-terminal tailBy similarityAdd BLAST272
Regioni5355 – 53704 X 4 AA tandem repeats of [GS]-S-R-[AR]Add BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi700 – 703Poly-Glu4
Compositional biasi1414 – 1417Poly-Glu4
Compositional biasi5264 – 5401Ser-richAdd BLAST138

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The C-terminal tail is required for phosphorylation by GSK3B and for microtubule-binding.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the plakin or cytolinker family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, SH3 domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0516, Eukaryota

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
D3ZHV2

Database of Orthologous Groups

More...
OrthoDBi
24858at2759

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00014, CH, 2 hits
cd00051, EFh, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.418.10, 2 hits
3.30.920.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001589, Actinin_actin-bd_CS
IPR001715, CH-domain
IPR036872, CH_dom_sf
IPR041615, Desmoplakin_SH3
IPR041573, Desmoplakin_Spectrin-like
IPR029926, Dystonin-like
IPR011992, EF-hand-dom_pair
IPR018247, EF_Hand_1_Ca_BS
IPR002048, EF_hand_dom
IPR003108, GAR_dom
IPR036534, GAR_dom_sf
IPR043197, Plakin
IPR001452, SH3_domain
IPR018159, Spectrin/alpha-actinin
IPR002017, Spectrin_repeat

The PANTHER Classification System

More...
PANTHERi
PTHR23169, PTHR23169, 4 hits
PTHR23169:SF24, PTHR23169:SF24, 4 hits

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00307, CH, 2 hits
PF13499, EF-hand_7, 1 hit
PF02187, GAS2, 1 hit
PF17902, SH3_10, 1 hit
PF00435, Spectrin, 18 hits
PF18373, Spectrin_like, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00033, CH, 2 hits
SM00054, EFh, 2 hits
SM00243, GAS2, 1 hit
SM00150, SPEC, 34 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF143575, SSF143575, 1 hit
SSF47473, SSF47473, 1 hit
SSF47576, SSF47576, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00019, ACTININ_1, 1 hit
PS00020, ACTININ_2, 1 hit
PS50021, CH, 2 hits
PS00018, EF_HAND_1, 2 hits
PS50222, EF_HAND_2, 2 hits
PS51460, GAR, 1 hit
PS50002, SH3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

D3ZHV2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSSDEETLS ERSCRSERSC RSERSYRSER SGSLSPCPPG DTLPWNLPLH
60 70 80 90 100
EQKKRKSQDS VLDPAERAVV RVADERDRVQ KKTFTKWVNK HLMKVRKHIN
110 120 130 140 150
DLYEDLRDGH NLISLLEVLS GIKLPREKGR MRFHRLQNVQ IALDFLKQRQ
160 170 180 190 200
VKLVNIRNDD ITDGNPKLTL GLIWTIILHF QISDIYISGE SGDMSAKEKL
210 220 230 240 250
LLWTQKVTAG YTGIKCTNFS SCWSDGKMFN ALIHRYRPDL VDMERVQIQS
260 270 280 290 300
NRENLEQAFE VAERLGVTRL LDAEDVDVPS PDEKSVITYV SSIYDAFPKV
310 320 330 340 350
PEGGEGISAT EVDSRWQEYQ SRVDSLIPWI RQHTILMSDK SFPQNPVELK
360 370 380 390 400
ALYNQYIHFK ETEILAKERE KGRIKELYKL LEVWIEFGRI KLPQGYHPNH
410 420 430 440 450
VEEEWGKLIV EMLEREKSLR PAVERLELLL QIANKIQNGA LNCEEKLTLA
460 470 480 490 500
KNTLQADAAH LESGQPVQCE SDVIMYIQEC EGLIRQLQVD LQILRDEKYY
510 520 530 540 550
QLEELAFRVM RLQDELVTLR LECTNLYRKG HFTSLELVPP STLTTTHLKA
560 570 580 590 600
EPLNKTTHSS STSWFRKPMT RTELVAISSS EDEGSLRFVY ELLSWVEEMQ
610 620 630 640 650
MKLERAEWGN DLPSVELQWE TQQHIHTSVE ELGSSVKEAR LYEGKMSQNF
660 670 680 690 700
HTSYVETLGK LETQYCKLKE TSSFRMRHLQ SLHKFVSKAT AELIWLNGKE
710 720 730 740 750
EEELACDWSD SNPNISAKKA YFSELTMELE GKQDVFRSLQ DTAELLSLEN
760 770 780 790 800
HPAKQTVEAY SAAVQSQLQW MKQLCLCVEQ HIKENAAYFQ FFSDARDLES
810 820 830 840 850
FLRNLQDSIK RKYTCDHNTS LSRLEDLLQD SMDEKEQLIQ SKSSVASLVG
860 870 880 890 900
RSKTIVQLKP RNPDHVLKST LSVKAICDYR QIEITICKND ECVLEDNSQR
910 920 930 940 950
TKWKVISPTG NEAMVPSVCL LIPPPNTEAI DVASRVEQSY QKVMALWHQL
960 970 980 990 1000
HINTKSLISW NYLRKDIDAV QTWNLEKLRS SAPGECHQVM KNLQAHYEDF
1010 1020 1030 1040 1050
LQDSHDSALF SVADRLRIEE EVEACKTHFQ QLMESMENED KEETLAKVYI
1060 1070 1080 1090 1100
SELKNIRLRL EECEQRLLKQ IQSSASSKTD RDARQDVALR IAEQEHVQED
1110 1120 1130 1140 1150
LKHLRSDLDA VSVKCTTFLQ QSPSGSSATT LRSELNLMVE KMDHVYGLSI
1160 1170 1180 1190 1200
VCLNKLKTID VIVRSIQDAE LLVKGYEIKL SQEEAVPADL SALESHRTTL
1210 1220 1230 1240 1250
QHWLSDVKDK NSVFSVLDEE ISKAKAVAEQ LHHRAAEPNL DLERYQEKGS
1260 1270 1280 1290 1300
QLQERWHRVI AQLETRQSEV ESIQEVLRDY RACHGTLIKW IEETTAQQEM
1310 1320 1330 1340 1350
MKPGQAEDSR VLSEQLSQQT ELFAEIERNQ TKLDQCQKLS QQYSTTVKDY
1360 1370 1380 1390 1400
ELQLMTYKAF VESQQKSPGK RRRMISSSDA ITQEFMDLRT RYTALVTLTT
1410 1420 1430 1440 1450
QHVKYISDAL RRLEEEEKVV EEEKQEHVEK VKDLLGWVST LARNTQGTTT
1460 1470 1480 1490 1500
SSRTSASTDI EKAILEQQVL AEELTTKKEQ VSEAIKTSQI FLAKHGHKLS
1510 1520 1530 1540 1550
EREKEQISEQ LCALNKTYHD LCDGSANQLQ QLQSELAQQT EQKTLQKQQD
1560 1570 1580 1590 1600
TCHKKLEDLR SWVRQAERAL ERHRGRASQQ ELSALQQNQS DLKDLQGDIQ
1610 1620 1630 1640 1650
NHSTSFATAV KDIEGFLEEN QNKLSPQELT ALREKLYQAK EQYEGLQDRT
1660 1670 1680 1690 1700
REAQKELEEA VTSALQQETE KSKAATELAE NRRKIDALLD WVTSVGSSDR
1710 1720 1730 1740 1750
QPQTSLPGTE QFSGACLEKQ TLDATDGCVD VNQVPEKLDR QYELMKARHQ
1760 1770 1780 1790 1800
ELLSQQQNFI VATQSAQSFL DQHSHNLTPE ERQMLQEKLG ELKEQYAASL
1810 1820 1830 1840 1850
AQSEAKLRQT QTLRDELQKF LQDHREFENW LERSENELDG MHTGGSSPEA
1860 1870 1880 1890 1900
LNSLLKRQGS FSEDVISHKG DLRFVTISGQ KVLETENNFE EGQEPSPTRN
1910 1920 1930 1940 1950
LVNEKLKDAT ERYTTLHSKC TRLGSHLNML LGQYQQFQSS ADSLQAWMLT
1960 1970 1980 1990 2000
CEASVEKLLS DTVASDPGIL QQQLATTKQL QEELAEHQVP VEKLQKAAHD
2010 2020 2030 2040 2050
LMDIEGEPSL DCTPIRETTE SIFSRFQSLS CSLAERSALL QKAIAQSQSV
2060 2070 2080 2090 2100
QESMESLLQS MKEVEQNLEG EQVAALSSGL IQEALANNMK LKQDIARQKS
2110 2120 2130 2140 2150
SLEATREMVT RFMETADGNS AAVLQDRLAE LSQRFHRLQL QQQEKESGLK
2160 2170 2180 2190 2200
KLLPQAETFE QLSSKLQQFV EHKNRLLASG NQPDQDIAHF SQHIQELTLE
2210 2220 2230 2240 2250
MEDQKENLGT LEHLVTALGS CGFALDLSQH QEKIQNLKKD FTELQKTVQE
2260 2270 2280 2290 2300
REKDASNCQE QLDEFRKLIR TFQKWLKETE GNVPPAETFV SAKELEKQIE
2310 2320 2330 2340 2350
HLKGLLDDWA GKGVLVEEIN TKGTALESLI MDITAPDSQA KTGSVLPSVG
2360 2370 2380 2390 2400
SSVGSVNGYH TCKDLTEIQC DMSDVNSKYD KLGDALRERQ ESLQTVLSRM
2410 2420 2430 2440 2450
EEVQKEASSV LQWLESKEEV LKGMDASLSP TKTETVKAQA ESNKAFLAEL
2460 2470 2480 2490 2500
EQNSPKIQKV KEALAGLLEA YPNSQEAENW RKMQEDLNSR WEKATEVTVA
2510 2520 2530 2540 2550
RQKQLEESAS HLACFQAAES QLRPWLMEKE LMMGVLGPLS IDPNMLNAQK
2560 2570 2580 2590 2600
QQVQFMLKEF EARRQQHEQL TEAAQGILTG PGDVSPSASQ VHKDLQSISQ
2610 2620 2630 2640 2650
KWVELTDKLN SRSTQIDQAI VKSTQYQDLL QDLSEKVKAV GQRLSGQSAI
2660 2670 2680 2690 2700
STQPDAVKQQ LEETSEIRSD LGQLDDEMKE AQTLCQELSL LIGEQYLKDE
2710 2720 2730 2740 2750
LKKRLETVAL PLQGLEDLAA DRMNRLQAAL ASTQQFQQMF DELRTWLDEK
2760 2770 2780 2790 2800
QSQQAKNCPI SAKLERLQSQ LQENEEFQKN LNQHSGSYEV IVAEGESLLL
2810 2820 2830 2840 2850
SVPPGEEKKT LQNQLVELKS HWEDLNKKTV DRQSRLKDCM QKAQKYQWHV
2860 2870 2880 2890 2900
EDLVPWIKDC KSKMSELQVT LDPVQLESSL LRSKAMLNEA EKRRSLLEIL
2910 2920 2930 2940 2950
NSAADILINS SEIDEDEIRD EKAGLNQNMD AITEELQAKT GSLEEMTQRL
2960 2970 2980 2990 3000
KEFQESFKNI EKKVEGAKHQ LEIFDALGSQ ACSNKNLEKL KAQREVLQAL
3010 3020 3030 3040 3050
DPQVDYLRDF TRGLVEDAPD GSDASPLVHQ AELAQQEFLE VKQRVNSSCL
3060 3070 3080 3090 3100
TMENKLEGIG QFHCRVREMF SQLADLDDEL DGMGAIGRDT DSLQSQIEDI
3110 3120 3130 3140 3150
RLFLNKIQAL RLDIEDSEAE CRKMLEEEGT LDLLGLKREL EALNKQCGKL
3160 3170 3180 3190 3200
TERGKARQEQ LELTLGRVED FYSKLKALND AATAAEEGEA LQWIVGTEVD
3210 3220 3230 3240 3250
VINQQLADFK MFQKEQVDPL QVKLQQVNGL GQGLIQSAGK TCDVQGLEHD
3260 3270 3280 3290 3300
MEEVNTRWNT LNKKVAQRIA QLQEALLHCG KFQDALEPLL SWLTDTEELI
3310 3320 3330 3340 3350
ANQKPPSAEY KVVKAQIQEQ KLLQRLLDDR KATVDMLQAE GGRIAQAAEL
3360 3370 3380 3390 3400
ADREKITGQL ESLECRWTEL LSKAAARQKQ LEDILVLAKQ FHETAEPISD
3410 3420 3430 3440 3450
FLSVTEKKLA NSEPVGTQTA KIHQQIIRHK ALEEEIENHA ADVQQAVKIG
3460 3470 3480 3490 3500
QSLSSLICPA EQGIMSEKLD SLQARYSEIQ DRCCRKASLL EQALFNARLF
3510 3520 3530 3540 3550
GEDEVEVLNW LAEVEDKLSA VFVKDYRQDV LQKQHADHLA LNEEIINRKK
3560 3570 3580 3590 3600
NVDQAIKNGQ ALLKQTTGEE VLLIQEKLDG IKTRYADITV TSSKALRTLE
3610 3620 3630 3640 3650
QARQLATKFH STYEELTGWL REVEEELAAS GGQSPTGEQI PQFQQRQKEL
3660 3670 3680 3690 3700
KKEVMEHRLV LDTVNEVSHA LLELVPWRAR EGLDKLVSDA NEQYKLVSDT
3710 3720 3730 3740 3750
VGQRVDEIDA AIQRSQQYEQ AADAELAWVA ETKRKLMALG PIRLEQDQTT
3760 3770 3780 3790 3800
AQLQVQKAFS IDIIRHKDSM DELFSHRGEI FSTCGEEQKA VLQEKTECLI
3810 3820 3830 3840 3850
QQYEAVSLLN SERYARLERA QVLVNQFWET YEELSPWAEE TLALIAQLPP
3860 3870 3880 3890 3900
PAVDHEQLRQ QQEEMRQLRE SIAEHKPHID KILKIGPQLK ELNPEEGKMV
3910 3920 3930 3940 3950
EEKYQKAENM YAQIKDEVRQ RALALDEAVS QSAQIAEFHD KIEPMLETLE
3960 3970 3980 3990 4000
NLSSRLRMPP LIPAEVDKIR ECISDNKSAT MELEKLQPSF EALKRRGEEL
4010 4020 4030 4040 4050
IGRSQGADKD LAAKEIQDKL DQMVFFWEDI KARSEEREIK FLDVLELAEK
4060 4070 4080 4090 4100
FWYDMAALLT TIKDTQEIVH DLESPGIDPS IIKQQVEAAE TIKEETDGLH
4110 4120 4130 4140 4150
EELEFIRILG ADLIFACGET EKPEVKKSID EMNNAWENLN RTWKERLEKL
4160 4170 4180 4190 4200
EDAMQAAVQY QDTLQAMFDW LDNTVIRLCT MPPVGTDLNT VKDQLNEMKE
4210 4220 4230 4240 4250
FKVEVYQQQI EMEKLNHQGE LMLKKATDET DRDIIREPLT ELKHLWENLG
4260 4270 4280 4290 4300
EKIAHRQHKL EGALLALGQF QHALEELMGW LTHTEELLDA QRPISGDPKV
4310 4320 4330 4340 4350
IEVELAKHHV LKNDVLAHQA TVETVNKAGN ELLESSAGDD ASSLRSRLET
4360 4370 4380 4390 4400
MNQCWESVLQ KTEEREQQLQ STLQQAQGFH SEIEEFLLEL NRMESQLSAS
4410 4420 4430 4440 4450
KPTGGLPETA REQLDAHMEL HSQLRAKEEI YNQLLDKGRL MLLSRGDSGS
4460 4470 4480 4490 4500
GSKTEQSVAL LEQKWHVVSS KVEERKSKLE EALSLATEFQ NSLQEFINWL
4510 4520 4530 4540 4550
TLAEQSLNIA SPPSLILNTV LSQIEEHKVF ANEVNAHRDQ IIELDQTGNQ
4560 4570 4580 4590 4600
LKFLSQKQDV VLIKNLLVSV QSRWEKVVQR SIERGRSLDD ARKRAKQFHE
4610 4620 4630 4640 4650
AWKKLIDWLE DAESHLDSEL EISNDPDKIK LQLSKHKEFQ KTLGGKQPVY
4660 4670 4680 4690 4700
DTTIRTGRAL KEKTLLADDA QKLDNLLGEV RDKWDTVCGK SVERQHKLEE
4710 4720 4730 4740 4750
ALLFSGQFMD ALQALVDWLY KVEPQLAEDQ PVHGDLDLVM NLMDAHKVFQ
4760 4770 4780 4790 4800
KELGKRTGTV QVLKRSGREL IESSRDDTTW VKGQLQELST RWDTVCKLSV
4810 4820 4830 4840 4850
SKQSRLEQAL KQAEEFRDTV HMLLEWLSEA EQTLRFRGAL PDDTEALQSL
4860 4870 4880 4890 4900
IDTHKEFMKK VEEKRVDVNA AVAMGEVILA VCHPDCITTI KHWITIIRAR
4910 4920 4930 4940 4950
FEEVLTWAKQ HQQRLETALS ELVANAELLE ELLAWIQWAE TTLIQRDQEP
4960 4970 4980 4990 5000
IPQNIDRVKA LITEHQSFME EMTRKQPDVD RVTKTYKRKN IEPTHAPFIE
5010 5020 5030 5040 5050
KSRSGSRKSL NQPTPPPMPI LSQSEAKNPR INQLSARWQQ VWLLALERQR
5060 5070 5080 5090 5100
KLNDALDRLE ELKEFANFDF DVWRKKYMRW MNHKKSRVMD FFRRIDKDQD
5110 5120 5130 5140 5150
GKITRQEFID GILASKFPTT KLEMTAVADI FDRDGDGYID YYEFVAALHP
5160 5170 5180 5190 5200
NKDAYRPTTD ADKIEDEVTR QVAQCKCAKR FQVEQIGENK YRFGDSQQLR
5210 5220 5230 5240 5250
LVRILRSTVM VRVGGGWMAL DEFLVKNDPC RARGRTNIEL REKFILPEGA
5260 5270 5280 5290 5300
SQGMTPFRSR GRRSKPSSRA ASPTRSSSSA SQSNHSCTSM PSSPATPASG
5310 5320 5330 5340 5350
TKVISSTGSK LKRPTPTFHS SRTSLAGDTS NSSSPASTGA KTNRADPKKS
5360 5370 5380 5390 5400
ASRPGSRAGS RAGSRASSRR GSDASDFDLL ETQSACSDTS ESSAAGGQGS
5410 5420 5430
SRRGLTKPSK IPTMSKKTTT ASPRTPCPKR
Length:5,430
Mass (Da):619,600
Last modified:April 20, 2010 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC15B030F89CD5FBF
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K9T4A0A0G2K9T4_RAT
Microtubule-actin cross-linking fac...
Macf1
7,384Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0G2JWA8A0A0G2JWA8_RAT
Microtubule-actin cross-linking fac...
Macf1
7,351Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M9MMM9M9MMM9_RAT
Microtubule-actin cross-linking fac...
Macf1
5,358Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0G2JU82A0A0G2JU82_RAT
Microtubule-actin cross-linking fac...
Macf1
5,358Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AC114512 Genomic DNA No translation available.
AC131172 Genomic DNA No translation available.

NCBI Reference Sequences

More...
RefSeqi
XP_017448990.1, XM_017593501.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
362587

UCSC genome browser

More...
UCSCi
RGD:1306057, rat

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC114512 Genomic DNA No translation available.
AC131172 Genomic DNA No translation available.
RefSeqiXP_017448990.1, XM_017593501.1

3D structure databases

SMRiD3ZHV2
ModBaseiSearch...

Protein-protein interaction databases

CORUMiD3ZHV2
STRINGi10116.ENSRNOP00000041065

PTM databases

CarbonylDBiD3ZHV2
iPTMnetiD3ZHV2

Proteomic databases

jPOSTiD3ZHV2
PaxDbiD3ZHV2
PeptideAtlasiD3ZHV2
PRIDEiD3ZHV2

Genome annotation databases

GeneIDi362587
UCSCiRGD:1306057, rat

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
23499
RGDi1306057, Macf1

Phylogenomic databases

eggNOGiKOG0516, Eukaryota
InParanoidiD3ZHV2
OrthoDBi24858at2759

Miscellaneous databases

Protein Ontology

More...
PROi
PR:D3ZHV2

Family and domain databases

CDDicd00014, CH, 2 hits
cd00051, EFh, 1 hit
Gene3Di1.10.418.10, 2 hits
3.30.920.20, 1 hit
InterProiView protein in InterPro
IPR001589, Actinin_actin-bd_CS
IPR001715, CH-domain
IPR036872, CH_dom_sf
IPR041615, Desmoplakin_SH3
IPR041573, Desmoplakin_Spectrin-like
IPR029926, Dystonin-like
IPR011992, EF-hand-dom_pair
IPR018247, EF_Hand_1_Ca_BS
IPR002048, EF_hand_dom
IPR003108, GAR_dom
IPR036534, GAR_dom_sf
IPR043197, Plakin
IPR001452, SH3_domain
IPR018159, Spectrin/alpha-actinin
IPR002017, Spectrin_repeat
PANTHERiPTHR23169, PTHR23169, 4 hits
PTHR23169:SF24, PTHR23169:SF24, 4 hits
PfamiView protein in Pfam
PF00307, CH, 2 hits
PF13499, EF-hand_7, 1 hit
PF02187, GAS2, 1 hit
PF17902, SH3_10, 1 hit
PF00435, Spectrin, 18 hits
PF18373, Spectrin_like, 1 hit
SMARTiView protein in SMART
SM00033, CH, 2 hits
SM00054, EFh, 2 hits
SM00243, GAS2, 1 hit
SM00150, SPEC, 34 hits
SUPFAMiSSF143575, SSF143575, 1 hit
SSF47473, SSF47473, 1 hit
SSF47576, SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS00019, ACTININ_1, 1 hit
PS00020, ACTININ_2, 1 hit
PS50021, CH, 2 hits
PS00018, EF_HAND_1, 2 hits
PS50222, EF_HAND_2, 2 hits
PS51460, GAR, 1 hit
PS50002, SH3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMACF1_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: D3ZHV2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: April 20, 2010
Last modified: August 12, 2020
This is version 73 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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