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Protein

Serine/threonine-protein kinase Nek8

Gene

Nek8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek8 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 8
Short name:
NimA-related protein kinase 8
Gene namesi
Name:Nek8
Synonyms:Jck
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1306897 Nek8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004244151 – 698Serine/threonine-protein kinase Nek8Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162Phosphothreonine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiD3ZGQ5
PRIDEiD3ZGQ5

Expressioni

Gene expression databases

BgeeiENSRNOG00000012866 Expressed in 9 organ(s), highest expression level in adult mammalian kidney

Interactioni

Subunit structurei

Interacts with PKD2; may regulate PKD2 targeting to the cilium. Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3 (PubMed:23793029). ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target it to the proximal ciliary axoneme. Interacts with ANKS3 (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017264

Structurei

3D structure databases

ProteinModelPortaliD3ZGQ5
SMRiD3ZGQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 258Protein kinasePROSITE-ProRule annotationAdd BLAST255
Repeati415 – 466RCC1 1Add BLAST52
Repeati467 – 518RCC1 2Add BLAST52
Repeati520 – 571RCC1 3Add BLAST52
Repeati585 – 636RCC1 4Add BLAST52
Repeati638 – 689RCC1 5Add BLAST52

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0589 Eukaryota
ENOG410Y7JF LUCA
GeneTreeiENSGT00760000118997
InParanoidiD3ZGQ5
KOiK20877
OMAiSAYAWGE
OrthoDBiEOG091G0GU4
PhylomeDBiD3ZGQ5
TreeFamiTF106472

Family and domain databases

Gene3Di2.130.10.30, 2 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00415 RCC1, 4 hits
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF50985 SSF50985, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50012 RCC1_3, 4 hits

Sequencei

Sequence statusi: Complete.

D3ZGQ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKYERIRVV GRGAFGIVHL CLRKADQKLV IIKQIPVEQM TKEERQAAQN
60 70 80 90 100
ECQVLKLLNH PNVIEYYENF LEDKALMIAM EYAPGGTLAE FIQKRCNSLL
110 120 130 140 150
EEETILHFFV QILLALHHVH THLILHRDLK TQNILLDKHR MVVKIGDFGI
160 170 180 190 200
SKILSSKSKA YTVVGTPCYI SPELCEGKPY NQKSDIWALG CVLYELASLK
210 220 230 240 250
RAFEAANLPA LVLKIMSGTF APISDRYSPE LRQLVLSLLS LEPAQRPPLS
260 270 280 290 300
HIMAQPLCIR ALLNIHTDVG SVRMRRAEKC LTPGTPMAPG STGSRATSAR
310 320 330 340 350
CRGVPRGPVR PAIPPPLSSV YAWGGGLSIP LRLPMPNTEV VQVAAGRTQK
360 370 380 390 400
AGVTRSGRLI LWEAPPLGTG GGTLLPGAVE LPQPQFVSRF LEGQSGVTIK
410 420 430 440 450
HVACGDLFTA CLTDRGIIMT FGSGSNGCLG HGSLTDISQP TIVEALLGYE
460 470 480 490 500
MVQVACGASH VLALSADGEL FAWGRGDGGR LGLGTRESHN CPQQVPMVPG
510 520 530 540 550
QEAQRVVCGI DCSMILTSPG RVLACGSNRF NKLGLDCLSL EEEPVPHQQV
560 570 580 590 600
EEALSFTPLG SAPLDRETLL CVDLGTAHSA AVTASGACYT FGSNQHGQLG
610 620 630 640 650
TSSRRVSRAP CRVQGLEGIK MVMVACGDAF TVAIGAEGEV YSWGKGARGR
660 670 680 690
LGRRDEDAGL PRPVQLDETH PYTVTSVSCC HGNTLLAVRS VTDEPVPP
Length:698
Mass (Da):75,254
Last modified:April 20, 2010 - v1
Checksum:i3846FCE2E3E53A0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06064754 Genomic DNA No translation available.
CH473948 Genomic DNA Translation: EDM05318.1
RefSeqiNP_001099274.1, NM_001105804.1
XP_006246995.1, XM_006246933.2
UniGeneiRn.144705

Genome annotation databases

EnsembliENSRNOT00000017264; ENSRNOP00000017264; ENSRNOG00000012866
GeneIDi287473
KEGGirno:287473

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06064754 Genomic DNA No translation available.
CH473948 Genomic DNA Translation: EDM05318.1
RefSeqiNP_001099274.1, NM_001105804.1
XP_006246995.1, XM_006246933.2
UniGeneiRn.144705

3D structure databases

ProteinModelPortaliD3ZGQ5
SMRiD3ZGQ5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017264

Proteomic databases

PaxDbiD3ZGQ5
PRIDEiD3ZGQ5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017264; ENSRNOP00000017264; ENSRNOG00000012866
GeneIDi287473
KEGGirno:287473

Organism-specific databases

CTDi284086
RGDi1306897 Nek8

Phylogenomic databases

eggNOGiKOG0589 Eukaryota
ENOG410Y7JF LUCA
GeneTreeiENSGT00760000118997
InParanoidiD3ZGQ5
KOiK20877
OMAiSAYAWGE
OrthoDBiEOG091G0GU4
PhylomeDBiD3ZGQ5
TreeFamiTF106472

Miscellaneous databases

PROiPR:D3ZGQ5

Gene expression databases

BgeeiENSRNOG00000012866 Expressed in 9 organ(s), highest expression level in adult mammalian kidney

Family and domain databases

Gene3Di2.130.10.30, 2 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00415 RCC1, 4 hits
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF50985 SSF50985, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50012 RCC1_3, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNEK8_RAT
AccessioniPrimary (citable) accession number: D3ZGQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: April 20, 2010
Last modified: September 12, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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