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Protein

Serine/threonine-protein kinase Nek7

Gene

Nek7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. releasing the autoinhibitory function of Tyr-97 (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATPPROSITE-ProRule annotation1
Sitei97AutoinhibitoryBy similarity1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 48ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-RNO-3301854 Nuclear Pore Complex (NPC) Disassembly

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek7 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 7
Short name:
NimA-related protein kinase 7
Gene namesi
Name:Nek7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1311160 Nek7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004128231 – 302Serine/threonine-protein kinase Nek7Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei195Phosphoserine; by NEK9By similarity1

Post-translational modificationi

Phosphorylation at Ser-195 required for its activation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiD3ZBE5
PeptideAtlasiD3ZBE5
PRIDEiD3ZBE5

PTM databases

iPTMnetiD3ZBE5
PhosphoSitePlusiD3ZBE5

Expressioni

Tissue specificityi

Highly expressed in the liver.1 Publication

Gene expression databases

BgeeiENSRNOG00000000657
ExpressionAtlasiD3ZBE5 baseline and differential

Interactioni

Subunit structurei

Monomer. Interacts with NEK9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000817

Structurei

3D structure databases

ProteinModelPortaliD3ZBE5
SMRiD3ZBE5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST266

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 33NTE motifBy similarityAdd BLAST14

Domaini

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 (By similarity).By similarity
The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0591 Eukaryota
ENOG410XNQP LUCA
GeneTreeiENSGT00760000118997
InParanoidiD3ZBE5
KOiK20876
PhylomeDBiD3ZBE5
TreeFamiTF105135

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

D3ZBE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEQPQGMQG PPVPQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR
60 70 80 90 100
ASCLLDGVPV ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS
110 120 130 140 150
FIEDNELNIV LELADAGDLS RMIKHFKKQK RLIPERTVWK YFVQLCSALD
160 170 180 190 200
HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSKT TAAHSLVGTP
210 220 230 240 250
YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLYSLCKKI
260 270 280 290 300
EQCDYPPLPS DHYSEELRQL VNICINPDPE KRPDIAYVYD VAKRMHACTA

SS
Length:302
Mass (Da):34,529
Last modified:April 20, 2010 - v1
Checksum:i1773BB6E19DFBEDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473958 Genomic DNA Translation: EDM09639.1
RefSeqiNP_001101816.1, NM_001108346.2
XP_006249988.1, XM_006249926.3
UniGeneiRn.211490

Genome annotation databases

EnsembliENSRNOT00000000817; ENSRNOP00000000817; ENSRNOG00000000657
GeneIDi360850
KEGGirno:360850
UCSCiRGD:1311160 rat

Similar proteinsi

Entry informationi

Entry nameiNEK7_RAT
AccessioniPrimary (citable) accession number: D3ZBE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: April 20, 2010
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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