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Protein

E3 ubiquitin-protein ligase CCNB1IP1

Gene

Ccnb1ip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over. Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 52RING-type; atypicalAdd BLAST43

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • blastocyst formation Source: MGI
  • chiasma assembly Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • reciprocal meiotic recombination Source: UniProtKB
  • spermatid development Source: MGI

Keywordsi

Molecular functionTransferase
Biological processMeiosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase CCNB1IP1 (EC:2.3.2.27By similarity)
Alternative name(s):
Cyclin-B1-interacting protein 1
RING-type E3 ubiquitin transferase CCNB1IP1Curated
Gene namesi
Name:Ccnb1ip1
Synonyms:Gm288, Hei10, Mei4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2685134 Ccnb1ip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Both male and female mice are sterile. Males do not make sperm and have much smaller testes, a characteristic of mutants with meiotic defects. Early stages of meiosis occur normally and full synapsis of homologous chromosomes (homologs) is achieved. However, crossover-specific recombination complexes do not assemble and crossing-overs fail. In spermatocytes, chromosomes fail to congress properly at the metaphase plate, leading to arrest and apoptosis before the first meiotic division. Mutant oocytes have a similar chromosomal phenotype but can undergo meiotic divisions and fertilization before arresting. During late meiotic prophase males, absence of Cdk2 and mismatch repair protein association from chromosome cores is correlated with the premature separation of bivalents at diplonema owing to lack of chiasmata.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77 – 100Missing in mei4; Sterility in both sexes due to meiotic defects. Add BLAST24

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004259011 – 276E3 ubiquitin-protein ligase CCNB1IP1Add BLAST276

Post-translational modificationi

Ubiquitinated; autoubiquitinated.By similarity
Phosphorylated by CDK1 on serine or threonine residues (in vitro).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiD3Z3K2
PRIDEiD3Z3K2

PTM databases

PhosphoSitePlusiD3Z3K2

Expressioni

Tissue specificityi

Expressed predominantly in the testes and 17 day embryos (corresponding to prophase I in females). Weakly or not expressed in other tissues.1 Publication

Developmental stagei

In spermatocytes, synaptonemal complex-associated Ccnb1ip1 foci are detected by early pachynema and their number peaks during midpachynema. In late-pachytene nuclei, Ccnb1ip1 foci number descrease. At the onset of diplonema, foci are no longer detected (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000071470 Expressed in 122 organ(s), highest expression level in oocyte
GenevisibleiD3Z3K2 MM

Interactioni

Subunit structurei

Interacts with CCNB1, UBE2L3 and NF2.By similarity

Protein-protein interaction databases

BioGridi232042, 35 interactors
STRINGi10090.ENSMUSP00000093622

Structurei

3D structure databases

ProteinModelPortaliD3Z3K2
SMRiD3Z3K2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili146 – 182Sequence analysisAdd BLAST37

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 52RING-type; atypicalAdd BLAST43

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IE9K Eukaryota
ENOG410XTEP LUCA
GeneTreeiENSGT00390000002849
HOGENOMiHOG000111677
InParanoidiD3Z3K2
KOiK10639
OMAiSHVFCDQ
OrthoDBiEOG091G0RN7
PhylomeDBiD3Z3K2
TreeFamiTF328863

Family and domain databases

InterProiView protein in InterPro
IPR001841 Znf_RING
PfamiView protein in Pfam
PF14634 zf-RING_5, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

D3Z3K2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLCEDMLLC NYRKCRIKLS GYAWVTACSH IFCDQHGSGE FSRSPAICPA
60 70 80 90 100
CNSTLSGKLD IVRTELSPSE EYKAMVLAGL RPEVVLDISS RALAFWTYQV
110 120 130 140 150
HQERLYQEYN FSKAENHLKQ MEKMYMQQIQ SKNIELTSMK GEVISMKKVL
160 170 180 190 200
EEYKKKFSDI SEKLMERNRQ YQKLQGLYDS LRLRNITIAS QEGSLEPGMI
210 220 230 240 250
PQSGVFGFPP GNNSKFSLDH IPVGNQGGGD EDVQFRPFFV CSPTAPEPIN
260 270
NFFSFASPSH EAEQQVCSRA FKAKRI
Length:276
Mass (Da):31,386
Last modified:April 20, 2010 - v1
Checksum:i8677CFEC187C1465
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2I3BPZ8A0A2I3BPZ8_MOUSE
E3 ubiquitin-protein ligase CCNB1IP...
Ccnb1ip1
16Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC027184 Genomic DNA No translation available.
CH466605 Genomic DNA Translation: EDL20823.1
CCDSiCCDS49477.1
RefSeqiNP_001104589.1, NM_001111119.1
UniGeneiMm.343880

Genome annotation databases

EnsembliENSMUST00000095932; ENSMUSP00000093622; ENSMUSG00000071470
GeneIDi239083
KEGGimmu:239083
UCSCiuc011zjw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC027184 Genomic DNA No translation available.
CH466605 Genomic DNA Translation: EDL20823.1
CCDSiCCDS49477.1
RefSeqiNP_001104589.1, NM_001111119.1
UniGeneiMm.343880

3D structure databases

ProteinModelPortaliD3Z3K2
SMRiD3Z3K2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232042, 35 interactors
STRINGi10090.ENSMUSP00000093622

PTM databases

PhosphoSitePlusiD3Z3K2

Proteomic databases

PaxDbiD3Z3K2
PRIDEiD3Z3K2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095932; ENSMUSP00000093622; ENSMUSG00000071470
GeneIDi239083
KEGGimmu:239083
UCSCiuc011zjw.1 mouse

Organism-specific databases

CTDi57820
MGIiMGI:2685134 Ccnb1ip1

Phylogenomic databases

eggNOGiENOG410IE9K Eukaryota
ENOG410XTEP LUCA
GeneTreeiENSGT00390000002849
HOGENOMiHOG000111677
InParanoidiD3Z3K2
KOiK10639
OMAiSHVFCDQ
OrthoDBiEOG091G0RN7
PhylomeDBiD3Z3K2
TreeFamiTF328863

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:D3Z3K2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071470 Expressed in 122 organ(s), highest expression level in oocyte
GenevisibleiD3Z3K2 MM

Family and domain databases

InterProiView protein in InterPro
IPR001841 Znf_RING
PfamiView protein in Pfam
PF14634 zf-RING_5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCIP1_MOUSE
AccessioniPrimary (citable) accession number: D3Z3K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: April 20, 2010
Last modified: November 7, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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