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Protein

Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2

Gene

Mthfd2l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei238NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi205 – 207NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandMagnesium, NAD

Enzyme and pathway databases

ReactomeiR-MMU-196757 Metabolism of folate and pterines
UniPathwayi
UPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2
Alternative name(s):
NADP-dependent methylenetetrahydrofolate dehydrogenase 2-like protein
Short name:
MTHFD2-like
Including the following 2 domains:
NAD-dependent methylenetetrahydrofolate dehydrogenase (EC:1.5.1.15)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Gene namesi
Name:Mthfd2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1915871 Mthfd2l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004133281 – 338Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2Add BLAST338

Proteomic databases

MaxQBiD3YZG8
PaxDbiD3YZG8
PeptideAtlasiD3YZG8
PRIDEiD3YZG8

PTM databases

PhosphoSitePlusiD3YZG8

Expressioni

Gene expression databases

BgeeiENSMUSG00000029376 Expressed in 223 organ(s), highest expression level in cardiac ventricle
ExpressionAtlasiD3YZG8 baseline and differential
GenevisibleiD3YZG8 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071578

Structurei

3D structure databases

ProteinModelPortaliD3YZG8
SMRiD3YZG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 93Substrate bindingBy similarity5
Regioni136 – 138Substrate bindingBy similarity3
Regioni314 – 318Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0089 Eukaryota
COG0190 LUCA
GeneTreeiENSGT00930000150894
HOGENOMiHOG000218242
InParanoidiD3YZG8
KOiK13403
OMAiTPKGIMR
OrthoDBiEOG091G0VB7
PhylomeDBiD3YZG8
TreeFamiTF323998

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00767 THF_DHG_CYH_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

D3YZG8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATRARGFSL LRGRLGRGPV RAPGVAERAW RGFGSSGRRH EAVIISGTNM
60 70 80 90 100
AKQIQKEIQQ GVESWIALGN RRPHLSIILV GDNPASHTYV RNKIKAASAV
110 120 130 140 150
GICSELIIKP KNVSQEELLD ITDQLNMDPR VSGILVQLPL PDHVDERTIC
160 170 180 190 200
NGIAPEKDVD GFHIINIGRL CLDQHSLIPA TASAVWEIIK RAGIETFGKN
210 220 230 240 250
VVVAGRSKNV GMPIAMLLHT DGEHERPGGD ATVTIAHRYT PREQLKAHTQ
260 270 280 290 300
LADIIIVAAG IPRLITADMV REGAAVIDVG INYIQDPVTG KTKLVGDVDF
310 320 330
EAVKKKASFI TPVPGGVGPM TVAMLLKNTL LAAKNIAY
Length:338
Mass (Da):36,438
Last modified:April 20, 2010 - v1
Checksum:i71A447A15C918D8E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YUR4A0A0J9YUR4_MOUSE
Probable bifunctional methylenetetr...
Mthfd2l
62Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109311 Genomic DNA No translation available.
AC157938 Genomic DNA No translation available.
CH466617 Genomic DNA Translation: EDL05299.1
CCDSiCCDS39145.1
RefSeqiNP_081064.1, NM_026788.1
UniGeneiMm.153030

Genome annotation databases

EnsembliENSMUST00000071652; ENSMUSP00000071578; ENSMUSG00000029376
GeneIDi665563
KEGGimmu:665563
UCSCiuc008ybp.2 mouse

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109311 Genomic DNA No translation available.
AC157938 Genomic DNA No translation available.
CH466617 Genomic DNA Translation: EDL05299.1
CCDSiCCDS39145.1
RefSeqiNP_081064.1, NM_026788.1
UniGeneiMm.153030

3D structure databases

ProteinModelPortaliD3YZG8
SMRiD3YZG8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071578

PTM databases

PhosphoSitePlusiD3YZG8

Proteomic databases

MaxQBiD3YZG8
PaxDbiD3YZG8
PeptideAtlasiD3YZG8
PRIDEiD3YZG8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071652; ENSMUSP00000071578; ENSMUSG00000029376
GeneIDi665563
KEGGimmu:665563
UCSCiuc008ybp.2 mouse

Organism-specific databases

CTDi441024
MGIiMGI:1915871 Mthfd2l

Phylogenomic databases

eggNOGiKOG0089 Eukaryota
COG0190 LUCA
GeneTreeiENSGT00930000150894
HOGENOMiHOG000218242
InParanoidiD3YZG8
KOiK13403
OMAiTPKGIMR
OrthoDBiEOG091G0VB7
PhylomeDBiD3YZG8
TreeFamiTF323998

Enzyme and pathway databases

UniPathwayi
UPA00193

ReactomeiR-MMU-196757 Metabolism of folate and pterines

Miscellaneous databases

PROiPR:D3YZG8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029376 Expressed in 223 organ(s), highest expression level in cardiac ventricle
ExpressionAtlasiD3YZG8 baseline and differential
GenevisibleiD3YZG8 MM

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00767 THF_DHG_CYH_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTD2L_MOUSE
AccessioniPrimary (citable) accession number: D3YZG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 20, 2010
Last modified: November 7, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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